Reviewed, UniProtKB/Swiss-Prot P07766 (CD3E_HUMAN)

Names and origin

Protein names

Recommended name:
    T-cell surface glycoprotein CD3 epsilon chain
Alternative name(s):
    T-cell surface antigen T3/Leu-4 epsilon chain
    CD_antigen=CD1e

Gene names

Name:	CD3E
Synonyms:	T3E

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	207 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The CD3 complex mediates signal transduction.
Subunit structure	The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta.
Subcellular location	Membrane; Single-pass type I membrane protein.
Sequence similarities	Contains 1 Ig-like (immunoglobulin-like) domain. Contains 1 ITAM domain.

Ontologies

Keywords
Cellular component	Membrane
Domain	Immunoglobulin domain Signal Transmembrane
Molecular function	Receptor
PTM	Phosphoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological process	G-protein coupled receptor protein signaling pathway Traceable author statement. Source: ProtInc positive regulation of T cell proliferation Inferred from mutant phenotype. Source: UniProtKB regulation of apoptosis Non-traceable author statement. Source: UniProtKB signal complex assembly Traceable author statement. Source: ProtInc
Cellular component	T cell receptor complex Non-traceable author statement. Source: UniProtKB external side of plasma membrane Inferred from direct assay. Source: MGI integral to plasma membrane Non-traceable author statement. Source: UniProtKB
Molecular function	SH3 domain binding Inferred from physical interaction. Source: UniProtKB T cell receptor binding Non-traceable author statement. Source: UniProtKB protein heterodimerization activity Inferred from physical interaction. Source: UniProtKB protein kinase binding Non-traceable author statement. Source: UniProtKB receptor signaling complex scaffold activity Non-traceable author statement. Source: UniProtKB receptor signaling protein activity Traceable author statement. Source: ProtInc transmembrane receptor activity Non-traceable author statement. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Molecule processing

Signal peptide

1 – 22

22

Chain

23 – 207

185

T-cell surface glycoprotein CD3 epsilon chain

Regions

Topological domain

23 – 126

104

Extracellular Potential

Transmembrane

127 – 152

26

Potential

Topological domain

153 – 207

55

Cytoplasmic Potential

Domain

32 – 112

81

Ig-like

Domain

178 – 205

28

ITAM

Amino acid modifications

Modified residue

188

1

Phosphotyrosine

Modified residue

199

1

Phosphotyrosine

Disulfide bond

49 ↔ 98

By similarity

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

207

Helix Strand Turn

Details...

Beta strand

37 – 41

5

Beta strand

44 – 48

5

Beta strand

57 – 61

5

Beta strand

64 – 69

6

Beta strand

75 – 78

4

Beta strand

81 – 84

4

Helix

89 – 92

4

Beta strand

94 – 100

7

Helix

105 – 107

3

Beta strand

110 – 116

7

Sequences

	Sequence		Length	Mass (Da)
	P07766-1 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: A1603D01CE9957D7	FASTA	207	23,147
	10 20 30 40 50 60 MQSGTHWRVL GLCLLSVGVW GQDGNEEMGG ITQTPYKVSI SGTTVILTCP QYPGSEILWQ 70 80 90 100 110 120 HNDKNIGGDE DDKNIGSDED HLSLKEFSEL EQSGYYVCYP RGSKPEDANF YLYLRARVCE 130 140 150 160 170 180 NCMEMDVMSV ATIVIVDICI TGGLLLLVYY WSKNRKAKAK PVTRGAGAGG RQRGQNKERP 190 200 PPVPNPDYEP IRKGQRDLYS GLNQRRI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of cDNA clones encoding the 20K non-glycosylated polypeptide chain of the human T-cell receptor/T3 complex." Gold D.P., Puck J.M., Pettey C.L., Cho M., Coligan J., Woody J.N., Terhorst C. Nature 321:431-434(1986) [PubMed: 3012357] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	Terhorst C. Submitted (JAN-1987) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	"Human CD3-epsilon gene contains three miniexons and is transcribed from a non-TATA promoter." Clevers H.C., Dunlap S., Wileman T.E., Terhorst C. Proc. Natl. Acad. Sci. U.S.A. 85:8156-8160(1988) [PubMed: 3267235] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Blood.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Blood.
[5]	"Profiling of tyrosine phosphorylation pathways in human cells using mass spectrometry." Salomon A.R., Ficarro S.B., Brill L.M., Brinker A., Phung Q.T., Ericson C., Sauer K., Brock A., Horn D.M., Schultz P.G., Peters E.C. Proc. Natl. Acad. Sci. U.S.A. 100:443-448(2003) [PubMed: 12522270] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-199, MASS SPECTROMETRY.
[6]	"Immunoaffinity profiling of tyrosine phosphorylation in cancer cells." Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J. Nat. Biotechnol. 23:94-101(2005) [PubMed: 15592455] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-188 AND TYR-199, MASS SPECTROMETRY.
[7]	"Quantitative phosphoproteome analysis using a dendrimer conjugation chemistry and tandem mass spectrometry." Tao W.A., Wollscheid B., O'Brien R., Eng J.K., Li X.-J., Bodenmiller B., Watts J.D., Hood L., Aebersold R. Nat. Methods 2:591-598(2005) [PubMed: 16094384] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-188 AND TYR-199, MASS SPECTROMETRY. Tissue: T-cell.
[8]	"Structural basis for Syk tyrosine kinase ubiquity in signal transduction pathways revealed by the crystal structure of its regulatory SH2 domains bound to a dually phosphorylated ITAM peptide." Fuetterer K., Wong J., Grucza R.A., Chan A.C., Waksman G. J. Mol. Biol. 281:523-537(1998) [PubMed: 9698567] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 186-203 IN COMPLEX WITH SYK, PHOSPHORYLATION AT TYR-188 AND TYR-199.

Web resources

CD3Ebase

CD3E mutation db

GeneReviews

Cross-references

Sequence databases

EMBLGenBankDDBJ

X03884 mRNA. Translation: CAA27516.1.
M23323 , M23317, M23318, M23319, M23320, M23321, L34846, M23322 Genomic DNA. Translation: AAA52295.1.
BC049847 mRNA. Translation: AAH49847.1.

PIR

A32069.

RefSeq

NP_000724.1.

UniGene

Hs.3003

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1A81	X-ray	3.00	B/D/F/H/J/L	186-203	[»]
1SY6	X-ray	2.10	A	21-118	[»]
1XIW	X-ray	1.90	A/E	23-126	[»]

DisProt

DP00506.

ModBase

Search...

Protein-protein interaction databases

IntAct

P07766.

PTM databases

PhosphoSite

P07766.

Proteomic databases

PeptideAtlas

P07766.

Genome annotation databases

Ensembl

ENSG00000198851. Homo sapiens. [Contig view]

GeneID

916.

KEGG

hsa:916.

Organism-specific databases

H-InvDB

HIX0026293.

HGNC

HGNC:1674. CD3E.

HPA

CAB000010.

MIM

186830. gene+phenotype.

PharmGKB

PA26216.

GenAtlas

Search...

GeneCards

Search...

GeneLynx

Search...

Phylogenomic databases

HOGENOM

P07766.

HOVERGEN

P07766.

Enzyme and pathway databases

Reactome

REACT_6900. Signaling in Immune System.

Gene expression databases

ArrayExpress

P07766.

CleanEx

HS_CD3E.

GermOnline

ENSG00000198851. Homo sapiens.

Family and domain databases

InterPro

IPR015477. CD3_epsilon.
IPR007110. Ig-like.
IPR013783. Ig-like_fold.
IPR003598. Ig_sub2.
IPR003110. Phos_rcpt_ITAM.
[Graphical view]

Gene3D

G3DSA:2.60.40.10. Ig-like_fold. 1 hit.

PANTHER

PTHR10506. CD3_epsilon. 1 hit.

Pfam

PF02189. ITAM. 1 hit.
[Graphical view]

SMART

SM00408. IGc2. 1 hit.
SM00077. ITAM. 1 hit.
[Graphical view]

PROSITE

PS50835. IG_LIKE. False negative.
PS51055. ITAM_1. 1 hit.
[Graphical view]

ProDom

P07766.
[Graphical view] [Entries sharing at least one domain]

BLOCKS

Search...

Other Resources

DrugBank

DB00075. Muromonab.

LinkHub

P07766.

SOURCE

Search...

ProtoNet

Search...

Entry information

Entry name

CD3E_HUMAN

Accession

Primary (citable) accession number: P07766

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	February 1, 1996
Last modified:	July 22, 2008
This is version 96 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)