Reviewed, UniProtKB/Swiss-Prot P08173 (ACM4_HUMAN)

Names and origin

Protein names

Recommended name:
    Muscarinic acetylcholine receptor M4

Gene names

Name:

CHRM4

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	479 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is inhibition of adenylate cyclase.
Subcellular location	Cell membrane; Multi-pass membrane protein. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell junction Cell membrane Membrane Postsynaptic cell membrane Synapse
Domain	Transmembrane
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein Phosphoprotein
Gene Ontology (GO)
Biological process	acetylcholine receptor signaling, muscarinic pathway Ref.3 Traceable author statement. Source: ProtInc cell proliferation Traceable author statement. Source: ProtInc
Cellular component	integral to plasma membrane Ref.1 Traceable author statement. Source: ProtInc
Molecular function	muscarinic acetylcholine receptor activity Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 479	479	Muscarinic acetylcholine receptor M4
Regions
	Topological domain	1 – 31	31	Extracellular Potential
	Transmembrane	32 – 54	23	1 Potential
	Topological domain	55 – 68	14	Cytoplasmic Potential
	Transmembrane	69 – 89	21	2 Potential
	Topological domain	90 – 106	17	Extracellular Potential
	Transmembrane	107 – 128	22	3 Potential
	Topological domain	129 – 148	20	Cytoplasmic Potential
	Transmembrane	149 – 171	23	4 Potential
	Topological domain	172 – 193	22	Extracellular Potential
	Transmembrane	194 – 216	23	5 Potential
	Topological domain	217 – 401	185	Cytoplasmic Potential
	Transmembrane	402 – 422	21	6 Potential
	Topological domain	423 – 436	14	Extracellular Potential
	Transmembrane	437 – 456	20	7 Potential
	Topological domain	457 – 479	23	Cytoplasmic Potential
Amino acid modifications
	Modified residue	459	1	Phosphothreonine Potential
	Modified residue	463	1	Phosphothreonine Potential
	Modified residue	477	1	Phosphothreonine Potential
	Glycosylation	8	1	N-linked (GlcNAc...) Potential
	Glycosylation	13	1	N-linked (GlcNAc...) Potential
	Disulfide bond	105 ↔ 185		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P08173-1 [UniParc]. Last modified September 19, 2002. Version 2. Checksum: 9C1B05B359A64289	FASTA	479	53,049
	10 20 30 40 50 60 MANFTPVNGS SGNQSVRLVT SSSHNRYETV EMVFIATVTG SLSLVTVVGN ILVMLSIKVN 70 80 90 100 110 120 RQLQTVNNYF LFSLACADLI IGAFSMNLYT VYIIKGYWPL GAVVCDLWLA LDYVVSNASV 130 140 150 160 170 180 MNLLIISFDR YFCVTKPLTY PARRTTKMAG LMIAAAWVLS FVLWAPAILF WQFVVGKRTV 190 200 210 220 230 240 PDNQCFIQFL SNPAVTFGTA IAAFYLPVVI MTVLYIHISL ASRSRVHKHR PEGPKEKKAK 250 260 270 280 290 300 TLAFLKSPLM KQSVKKPPPG EAAREELRNG KLEEAPPPAL PPPPRPVADK DTSNESSSGS 310 320 330 340 350 360 ATQNTKERPA TELSTTEATT PAMPAPPLQP RALNPASRWS KIQIVTKQTG NECVTAIEIV 370 380 390 400 410 420 PATPAGMRPA ANVARKFASI ARNQVRKKRQ MAARERKVTR TIFAILLAFI LTWTPYNVMV 430 440 450 460 470 LVNTFCQSCI PDTVWSIGYW LCYVNSTINP ACYALCNATF KKTFRHLLLC QYRNIGTAR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a family of muscarinic acetylcholine receptor genes." Bonner T.I., Buckley N.J., Young A.C., Brann M.R. Science 237:527-532(1987) [PubMed: 3037705] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Cloning and expression of the human and rat m5 muscarinic acetylcholine receptor genes." Bonner T.I., Young A.C., Brann M.R., Buckley N.J. Neuron 1:403-410(1988) [PubMed: 3272174] [Abstract] Cited for: SEQUENCE REVISION TO 258 AND 261-266.
[3]	"Distinct primary structures, ligand-binding properties and tissue-specific expression of four human muscarinic acetylcholine receptors." Peralta E.G., Ashkenazi A., Winslow J.W., Smith D.H., Ramachandran J., Capon D.J. EMBO J. 6:3923-3929(1987) [PubMed: 3443095] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Puhl H.L. III, Ikeda S.R., Aronstam R.S. Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M16405 Genomic DNA. Translation: AAA51571.2. X15265 Genomic DNA. Translation: CAA33336.1. AF498918 mRNA. Translation: AAM18941.1. BC095546 mRNA. Translation: AAH95546.1. BC119775 mRNA. Translation: AAI19776.1. BC119817 mRNA. Translation: AAI19818.1.
PIR	S10127.
RefSeq	NP_000732.2.
UniGene	Hs.248100
3D structure databases
HSSP	HSSP built from PDB template 1BOJ based on UniProtKB P02699.
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
Genome annotation databases
Ensembl	ENSG00000180720. Homo sapiens. [Contig view]
GeneID	1132.
KEGG	hsa:1132.
Organism-specific databases
H-InvDB	HIX0035896.
HGNC	HGNC:1953. CHRM4.
MIM	118495. gene.
PharmGKB	PA26485.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOVERGEN	P08173.
Gene expression databases
CleanEx	HS_CHRM4.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_supfam. IPR001432. Musac_M4_rcpt. IPR000995. Musac_rcpt. [Graphical view]
PANTHER	PTHR19266:SF77. Musac_M4_rcpt. 1 hit.
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR00243. MUSCARINICR. PR00541. MUSCRINICM4R.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProDom	P08173. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
DrugBank	DB00572. Atropine. DB00767. Benzquinamide. DB00785. Cryptenamine. DB00725. Homatropine Methylbromide. DB01403. Methotrimeprazine. DB00340. Metixene. DB00334. Olanzapine. DB00387. Procyclidine. DB00420. Promazine. DB01069. Promethazine. DB00777. Propiomazine. DB00372. Thiethylperazine. DB00809. Tropicamide.
SOURCE	Search...
ProtoNet	Search...

Entry information

Entry name

ACM4_HUMAN

Accession

Primary (citable) accession number: P08173
Secondary accession number(s): Q0VD60, Q4VBK7

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	September 19, 2002
Last modified:	July 22, 2008
This is version 82 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)