Reviewed, UniProtKB/Swiss-Prot P08482 (ACM1_RAT)

Names and origin

Protein names

Recommended name:
    Muscarinic acetylcholine receptor M1

Gene names

Name:	Chrm1
Synonyms:	Chrm-1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	460 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.
Subunit structure	Interacts with GPRASP2 By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell junction Cell membrane Membrane Postsynaptic cell membrane Synapse
Domain	Transmembrane
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 460	460	Muscarinic acetylcholine receptor M1
Regions
	Topological domain	1 – 24	24	Extracellular Potential
	Transmembrane	25 – 47	23	1 Potential
	Topological domain	48 – 61	14	Cytoplasmic Potential
	Transmembrane	62 – 82	21	2 Potential
	Topological domain	83 – 99	17	Extracellular Potential
	Transmembrane	100 – 121	22	3 Potential
	Topological domain	122 – 141	20	Cytoplasmic Potential
	Transmembrane	142 – 164	23	4 Potential
	Topological domain	165 – 186	22	Extracellular Potential
	Transmembrane	187 – 209	23	5 Potential
	Topological domain	210 – 366	157	Cytoplasmic Potential
	Transmembrane	367 – 387	21	6 Potential
	Topological domain	388 – 401	14	Extracellular Potential
	Transmembrane	402 – 421	20	7 Potential
	Topological domain	422 – 460	39	Cytoplasmic Potential
Sites
	Binding site	105	1	Antagonist
Amino acid modifications
	Modified residue	428	1	Phosphothreonine Potential
	Modified residue	451	1	Phosphoserine Potential
	Modified residue	455	1	Phosphothreonine Potential
	Modified residue	457	1	Phosphoserine Potential
	Glycosylation	2	1	N-linked (GlcNAc...) Probable
	Glycosylation	12	1	N-linked (GlcNAc...) Probable
	Disulfide bond	98 ↔ 178		Probable

Sequences

	Sequence		Length	Mass (Da)
	P08482-1 [UniParc]. Last modified August 1, 1988. Version 1. Checksum: 527573ED8FF7C317	FASTA	460	51,369
	10 20 30 40 50 60 MNTSVPPAVS PNITVLAPGK GPWQVAFIGI TTGLLSLATV TGNLLVLISF KVNTELKTVN 70 80 90 100 110 120 NYFLLSLACA DLIIGTFSMN LYTTYLLMGH WALGTLACDL WLALDYVASN ASVMNLLLIS 130 140 150 160 170 180 FDRYFSVTRP LSYRAKRTPR RAALMIGLAW LVSFVLWAPA ILFWQYLVGE RTVLAGQCYI 190 200 210 220 230 240 QFLSQPIITF GTAMAAFYLP VTVMCTLYWR IYRETENRAR ELAALQGSET PGKGGGSSSS 250 260 270 280 290 300 SERSQPGAEG SPESPPGRCC RCCRAPRLLQ AYSWKEEEEE DEGSMESLTS SEGEEPGSEV 310 320 330 340 350 360 VIKMPMVDSE AQAPTKQPPK SSPNTVKRPT KKGRDRGGKG QKPRGKEQLA KRKTFSLVKE 370 380 390 400 410 420 KKAARTLSAI LLAFILTWTP YNIMVLVSTF CKDCVPETLW ELGYWLCYVN STVNPMCYAL 430 440 450 460 CNKAFRDTFR LLLLCRWDKR RWRKIPKRPG SVHRTPSRQC

References

[1]	"Identification of a family of muscarinic acetylcholine receptor genes." Bonner T.I., Buckley N.J., Young A.C., Brann M.R. Science 237:527-532(1987) [PubMed: 3037705] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The molecular properties of the M1 muscarinic receptor and its regulation of cytosolic calcium in a eukaryotic gene expression system." Lai J., Smith T.L., Mei L., Ikeda M., Fujiwara Y., Gomez J., Halonen M., Roeske W.R., Yamamura H.I. Adv. Exp. Med. Biol. 287:313-330(1991) [PubMed: 1759615] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Muscarinic acetylcholine receptors. Peptide sequencing identifies residues involved in antagonist binding and disulfide bond formation." Kurtenbach E., Curtis C.A.M., Pedder E.K., Aitken A., Harris A.C.M., Hulme E.C. J. Biol. Chem. 265:13702-13708(1990) [PubMed: 2380182] [Abstract] Cited for: PROTEIN SEQUENCE OF 63-124.
[4]	"Site-directed mutagenesis of the rat m1 muscarinic acetylcholine receptor. Role of conserved cysteines in receptor function." Savarese T.M., Wang C.-D., Fraser C.M. J. Biol. Chem. 267:11439-11448(1992) [PubMed: 1317867] [Abstract] Cited for: MUTAGENESIS OF CYSTEINE RESIDUES.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M16406 Genomic DNA. Translation: AAA40660.1. S73971 Genomic DNA. Translation: AAB20705.1.
PIR	A29514. A94518.
RefSeq	NP_542951.1.
UniGene	Rn.119395
3D structure databases
HSSP	HSSP built from PDB template 1HLL based on UniProtKB P08913.
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
Genome annotation databases
Ensembl	ENSRNOG00000018385. Rattus norvegicus. [Contig view]
GeneID	25229.
KEGG	rno:25229.
Organism-specific databases
RGD	2342. Chrm1.
Phylogenomic databases
HOVERGEN	P08482.
Gene expression databases
ArrayExpress	P08482.
GermOnline	ENSRNOG00000018385. Rattus norvegicus.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_supfam. IPR002228. Musac_M1_rcpt. IPR000995. Musac_rcpt. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR00243. MUSCARINICR. PR00538. MUSCRINICM1R.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProDom	P08482. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

ACM1_RAT

Accession

Primary (citable) accession number: P08482

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	August 1, 1988
Last modified:	July 22, 2008
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)