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Reviewed, UniProtKB/Swiss-Prot P08483 (ACM3_RAT)

Last modified July 22, 2008. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Muscarinic acetylcholine receptor M3
Gene names
Name: Chrm3
Synonyms: Chrm-3
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length589 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.

Subcellular location

Cell membrane; Multi-pass membrane proteinBy similarity. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane proteinBy similarity. Basolateral cell membrane; Multi-pass membrane proteinBy similarity.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical view

Molecule processing

Chain1 – 589589Muscarinic acetylcholine receptor M3

Regions

Topological domain1 – 6666Extracellular Potential
Transmembrane67 – 90241 Potential
Topological domain91 – 10313Cytoplasmic Potential
Transmembrane104 – 124212 Potential
Topological domain125 – 14117Extracellular Potential
Transmembrane142 – 163223 Potential
Topological domain164 – 18320Cytoplasmic Potential
Transmembrane184 – 206234 Potential
Topological domain207 – 22822Extracellular Potential
Transmembrane229 – 251235 Potential
Topological domain252 – 491240Cytoplasmic Potential
Transmembrane492 – 512216 Potential
Topological domain513 – 52614Extracellular Potential
Transmembrane527 – 546207 Potential
Topological domain547 – 58943Cytoplasmic Potential
Motif274 – 2807Basolateral sorting signal By similarity

Sites

Binding site1471Antagonist By similarity

Amino acid modifications

Glycosylation61N-linked (GlcNAc...) Potential
Glycosylation151N-linked (GlcNAc...) Potential
Glycosylation411N-linked (GlcNAc...) Potential
Glycosylation481N-linked (GlcNAc...) Potential
Glycosylation521N-linked (GlcNAc...) Potential
Disulfide bond140 ↔ 220 By similarity

Experimental info

Mutagenesis1481Y → A: Decreased affinity for acetylcholine
Mutagenesis2311T → A: Decreased affinity for acetylcholine
Mutagenesis2341T → A: Strongly decreased affinity for acetylcholine
Mutagenesis5061Y → F: Decreased affinity for acetylcholine
Mutagenesis5291Y → F: Decreased affinity for acetylcholine
Mutagenesis5331Y → F: Decreased affinity for acetylcholine
Sequence conflict1841A → R in AAA40661 and AAA40662. Ref.1
Sequence conflict1841A → R in BAA36839. Ref.4
Sequence conflict5161C → R in AAA40659. Ref.3
Sequence conflict5561T → M in AAA40659. Ref.3

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Sequences

Sequence LengthMass (Da)Tools
P08483-1 [UniParc].

Last modified November 1, 1988. Version 1.
Checksum: 9A5EF2FA653C830A

FASTA58966,066
        10         20         30         40         50         60 
MTLHSNSTTS PLFPNISSSW VHSPSEAGLP LGTVTQLGSY NISQETGNFS SNDTSSDPLG 

        70         80         90        100        110        120 
GHTIWQVVFI AFLTGFLALV TIIGNILVIV AFKVNKQLKT VNNYFLLSLA CADLIIGVIS 

       130        140        150        160        170        180 
MNLFTTYIIM NRWALGNLAC DLWLSIDYVA SNASVMNLLV ISFDRYFSIT RPLTYRAKRT 

       190        200        210        220        230        240 
TKRAGVMIGL AWVISFVLWA PAILFWQYFV GKRTVPPGEC FIQFLSEPTI TFGTAIAAFY 

       250        260        270        280        290        300 
MPVTIMTILY WRIYKETEKR TKELAGLQAS GTEAEAENFV HPTGSSRSCS SYELQQQGVK 

       310        320        330        340        350        360 
RSSRRKYGRC HFWFTTKSWK PSAEQMDQDH SSSDSWNNND AAASLENSAS SDEEDIGSET 

       370        380        390        400        410        420 
RAIYSIVLKL PGHSSILNST KLPSSDNLQV SNEDLGTVDV ERNAHKLQAQ KSMGDGDNCQ 

       430        440        450        460        470        480 
KDFTKLPIQL ESAVDTGKTS DTNSSADKTT ATLPLSFKEA TLAKRFALKT RSQITKRKRM 

       490        500        510        520        530        540 
SLIKEKKAAQ TLSAILLAFI ITWTPYNIMV LVNTFCDSCI PKTYWNLGYW LCYINSTVNP 

       550        560        570        580 
VCYALCNKTF RTTFKTLLLC QCDKRKRRKQ QYQQRQSVIF HKRVPEQAL

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References

[1]"Identification of a family of muscarinic acetylcholine receptor genes."
Bonner T.I., Buckley N.J., Young A.C., Brann M.R.
Science 237:527-532(1987) [PubMed: 3037705] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[2]"Cloning and expression of the human and rat m5 muscarinic acetylcholine receptor genes."
Bonner T.I., Young A.C., Brann M.R., Buckley N.J.
Neuron 1:403-410(1988) [PubMed: 3272174] [Abstract]
Cited for: SEQUENCE REVISION TO 184.
[3]"A novel subtype of muscarinic receptor identified by homology screening."
Braun T., Schofield P.R., Shivers B.D., Pritchett D.B., Seeburg P.H.
Biochem. Biophys. Res. Commun. 149:125-132(1987) [PubMed: 3120722] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[4]"Molecular cloning of m3 muscarinic acetylcholine receptor in rat iris."
Furuta M., Ohya S., Imaizumi Y., Watanabe M.
J. Smooth Muscle Res. 34:111-122(1998) [PubMed: 9972520] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Iris.
[5]"Muscarinic acetylcholine receptors. Peptide sequencing identifies residues involved in antagonist binding and disulfide bond formation."
Kurtenbach E., Curtis C.A.M., Pedder E.K., Aitken A., Harris A.C.M., Hulme E.C.
J. Biol. Chem. 265:13702-13708(1990) [PubMed: 2380182] [Abstract]
Cited for: PROTEIN SEQUENCE OF 104-166.
[6]"Site-directed mutagenesis of the m3 muscarinic receptor: identification of a series of threonine and tyrosine residues involved in agonist but not antagonist binding."
Wess J., Gdula D., Brann M.R.
EMBO J. 10:3729-3734(1991) [PubMed: 1657592] [Abstract]
Cited for: MUTAGENESIS OF TYR-148; THR-231; THR-234; TYR-506; TYR-529 AND TYR-533.
[7]"Role of conserved threonine and tyrosine residues in acetylcholine binding and muscarinic receptor activation. A study with m3 muscarinic receptor point mutants."
Wess J., Maggio R., Palmer J.R., Vogel Z.
J. Biol. Chem. 267:19313-19319(1992) [PubMed: 1527051] [Abstract]
Cited for: MUTAGENESIS OF THR-234 AND TYR-506.

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Cross-references

Sequence databases

M16407 mRNA. Translation: AAA40661.1. Sequence problems.
M16408 Genomic DNA. Translation: AAA40662.1. Sequence problems.
M18088 mRNA. Translation: AAA40659.1.
M62826 Genomic DNA. Translation: AAA41553.1.
AB017656 mRNA. Translation: BAA36839.1.
PIRA29476.
B29514. B94518.
RefSeqNP_036659.1.
UniGeneRn.87735

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
2AMKmodel-A56-560[»]
ModBaseSearch...

Protein family/group databases

GPCRDBSearch...

Genome annotation databases

EnsemblENSRNOG00000014639. Rattus norvegicus. [Contig view]
GeneID24260.
KEGGrno:24260.

Organism-specific databases

RGD2343. Chrm3.

Phylogenomic databases

HOVERGENP08483.

Gene expression databases

ArrayExpressP08483.
GermOnlineENSRNOG00000014639. Rattus norvegicus.

Family and domain databases

InterProIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_supfam.
IPR001183. Musac_M3_rcpt.
IPR000995. Musac_rcpt.
[Graphical view]
PANTHERPTHR19266:SF37. Musac_M3_rcpt. 1 hit.
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00237. GPCRRHODOPSN.
PR00243. MUSCARINICR.
PR00540. MUSCRINICM3R.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProDomP08483.
[Graphical view] [Entries sharing at least one domain]
BLOCKSSearch...

Other Resources

ProtoNetSearch...

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Entry information

Entry nameACM3_RAT
AccessionPrimary (citable) accession number: P08483
Secondary accession number(s): Q9QWK9
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: November 1, 1988
Last modified: July 22, 2008
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

UniProtKB secondary accession numbers

Index of UniProtKB secondary accession numbers

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents