Reviewed, UniProtKB/Swiss-Prot P08485 (ACM4_RAT)

Names and origin

Protein names

Recommended name:
    Muscarinic acetylcholine receptor M4

Gene names

Name:	Chrm4
Synonyms:	Chrm-4

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	478 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is inhibition of adenylate cyclase.
Subcellular location	Cell membrane; Multi-pass membrane protein. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell junction Cell membrane Membrane Postsynaptic cell membrane Synapse
Domain	Transmembrane
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein Phosphoprotein
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 478	478	Muscarinic acetylcholine receptor M4
Regions
	Topological domain	1 – 30	30	Extracellular Potential
	Transmembrane	31 – 53	23	1 Potential
	Topological domain	54 – 67	14	Cytoplasmic Potential
	Transmembrane	68 – 88	21	2 Potential
	Topological domain	89 – 105	17	Extracellular Potential
	Transmembrane	106 – 127	22	3 Potential
	Topological domain	128 – 147	20	Cytoplasmic Potential
	Transmembrane	148 – 170	23	4 Potential
	Topological domain	171 – 192	22	Extracellular Potential
	Transmembrane	193 – 215	23	5 Potential
	Topological domain	216 – 400	185	Cytoplasmic Potential
	Transmembrane	401 – 421	21	6 Potential
	Topological domain	422 – 435	14	Extracellular Potential
	Transmembrane	436 – 455	20	7 Potential
	Topological domain	456 – 478	23	Cytoplasmic Potential
Amino acid modifications
	Modified residue	458	1	Phosphothreonine Potential
	Modified residue	462	1	Phosphothreonine Potential
	Modified residue	476	1	Phosphothreonine Potential
	Glycosylation	8	1	N-linked (GlcNAc...) Potential
	Glycosylation	13	1	N-linked (GlcNAc...) Potential
	Disulfide bond	104 ↔ 184		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P08485-1 [UniParc]. Last modified August 1, 1988. Version 1. Checksum: 2551FF5F77B1A56C	FASTA	478	52,921
	10 20 30 40 50 60 MXNFTPVNGS SANQSVRLVT AAHNHLETVE MVFIATVTGS LSLVTVVGNI LVMLSIKVNR 70 80 90 100 110 120 QLQTVNNYFL FSLGCADLII GAFSMNLYTL YIIKGYWPLG AVVCDLWLAL DYVVSNASVM 130 140 150 160 170 180 NLLIISFDRY FCVTKPLTYP ARRTTKMAGL MIAAAWVLSF VLWAPAILFW QFVVGKRTVP 190 200 210 220 230 240 DNQCFIQFLS NPAVTFGTAI AAFYLPVVIM TVLYIHISLA SRSRVHKHRP EGPKEKKAKT 250 260 270 280 290 300 LAFLKSPLMK PSIKKPPPGG ASREELRNGK LEEAPPPALP PPPRPVPDKD TSNESSSGSA 310 320 330 340 350 360 TQNTKERPPT ELSTAEATTP ALPAPTLQPR TLNPASKWSK IQIVTKQTGN ECVTAIEIVP 370 380 390 400 410 420 ATPAGMRPAA NVARKFASIA RNQVRKKRQM AARERKVTRT IFAILLAFIL TWTPYNVMVL 430 440 450 460 470 VNTFCQSCIP ERVWSIGYWL CYVNSTINPA CYALCNATFK KTFRHLLLCQ YRNIGTAR

References

[1]	"Identification of a family of muscarinic acetylcholine receptor genes." Bonner T.I., Buckley N.J., Young A.C., Brann M.R. Science 237:527-532(1987) [PubMed: 3037705] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M16409 mRNA. Translation: AAA40663.1. Sequence problems.
PIR	C29514. C94518.
RefSeq	NP_113735.1.
UniGene	Rn.10676
3D structure databases
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
Genome annotation databases
Ensembl	ENSRNOG00000017556. Rattus norvegicus. [Contig view]
GeneID	25111.
KEGG	rno:25111.
Organism-specific databases
RGD	2344. Chrm4.
Phylogenomic databases
HOVERGEN	P08485.
Gene expression databases
ArrayExpress	P08485.
GermOnline	ENSRNOG00000017556. Rattus norvegicus.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_supfam. IPR001432. Musac_M4_rcpt. IPR000995. Musac_rcpt. [Graphical view]
PANTHER	PTHR19266:SF77. Musac_M4_rcpt. 1 hit.
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR00243. MUSCARINICR. PR00541. MUSCRINICM4R.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProDom	P08485. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

ACM4_RAT

Accession

Primary (citable) accession number: P08485

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	August 1, 1988
Last modified:	July 22, 2008
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)