Reviewed, UniProtKB/Swiss-Prot P08571 (CD14_HUMAN)

Names and origin

Protein names

Recommended name:
    Monocyte differentiation antigen CD14
Alternative name(s):
    Myeloid cell-specific leucine-rich glycoprotein
    CD_antigen=CD14
Cleaved into the following 2 chains:
    1- Recommended name:
            Monocyte differentiation antigen CD14, urinary form
    2- Recommended name:
            Monocyte differentiation antigen CD14, membrane-bound form

Gene names

Name:

CD14

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	375 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.
Subunit structure	Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, MD-2 and TLR4.
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor.
Tissue specificity	Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.
Sequence similarities	Contains 11 LRR (leucine-rich) repeats.

Ontologies

Keywords
Biological process	Immune response Inflammatory response
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	Leucine-rich repeat Repeat Signal
PTM	GPI-anchor Glycoprotein Lipoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	apoptosis Traceable author statement. Source: ProtInc cell surface receptor linked signal transduction Traceable author statement. Source: ProtInc phagocytosis Traceable author statement. Source: ProtInc
Cellular component	extracellular region Inferred from Experiment. Source: Reactome plasma membrane Traceable author statement. Source: ProtInc
Molecular function	opsonin receptor activity Traceable author statement. Source: UniProtKB peptidoglycan receptor activity Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Signal peptide	1 – 19	19
	Chain	20 – 367	348	Monocyte differentiation antigen CD14, urinary form
	Chain	20 – 345	326	Monocyte differentiation antigen CD14, membrane-bound form
	Propeptide	346 – 375	30	Removed in mature form Potential
Regions
	Repeat	54 – 82	29	LRR 1
	Repeat	83 – 118	36	LRR 2
	Repeat	119 – 144	26	LRR 3
	Repeat	145 – 172	28	LRR 4
	Repeat	173 – 196	24	LRR 5
	Repeat	197 – 224	28	LRR 6
	Repeat	225 – 251	27	LRR 7
	Repeat	252 – 278	27	LRR 8
	Repeat	279 – 299	21	LRR 9
	Repeat	300 – 321	22	LRR 10
	Repeat	322 – 349	28	LRR 11
Amino acid modifications
	Lipidation	345	1	GPI-anchor amidated asparagine Potential
	Glycosylation	37	1	N-linked (GlcNAc...) Potential
	Glycosylation	151	1	N-linked (GlcNAc...)
	Glycosylation	282	1	N-linked (GlcNAc...)
	Glycosylation	323	1	N-linked (GlcNAc...) Potential
	Disulfide bond	25 ↔ 36		By similarity
	Disulfide bond	34 ↔ 51		By similarity
	Disulfide bond	187 ↔ 217		By similarity
	Disulfide bond	241 ↔ 272		By similarity
Natural variations
	Natural variant	204	1	N → D: dbSNP rs2228049.
Experimental info
	Sequence conflict	77	1	A → G Ref.3
	Sequence conflict	187	1	C → Y Ref.1 Ref.2
	Sequence conflict	303	1	D → E in AAC83816. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P08571-1 [UniParc]. Last modified March 27, 2002. Version 2. Checksum: 1746CDB41F394F8D	FASTA	375	40,076
	10 20 30 40 50 60 MERASCLLLL LLPLVHVSAT TPEPCELDDE DFRCVCNFSE PQPDWSEAFQ CVSAVEVEIH 70 80 90 100 110 120 AGGLNLEPFL KRVDADADPR QYADTVKALR VRRLTVGAAQ VPAQLLVGAL RVLAYSRLKE 130 140 150 160 170 180 LTLEDLKITG TMPPLPLEAT GLALSSLRLR NVSWATGRSW LAELQQWLKP GLKVLSIAQA 190 200 210 220 230 240 HSPAFSCEQV RAFPALTSLD LSDNPGLGER GLMAALCPHK FPAIQNLALR NTGMETPTGV 250 260 270 280 290 300 CAALAAAGVQ PHSLDLSHNS LRATVNPSAP RCMWSSALNS LNLSFAGLEQ VPKGLPAKLR 310 320 330 340 350 360 VLDLSCNRLN RAPQPDELPE VDNLTLDGNP FLVPGTALPH EGSMNSGVVP ACARSTLSVG 370 VSGTLVLLQG ARGFA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the gene encoding the monocyte differentiation antigen, CD14." Ferrero E., Goyert S.M. Nucleic Acids Res. 16:4173-4173(1988) [PubMed: 2453848] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Lymphocyte.
[2]	"The monocyte differentiation antigen, CD14, is anchored to the cell membrane by a phosphatidylinositol linkage." Haziot A., Chen S., Ferrero E., Low M.G., Silber R., Goyert S.M. J. Immunol. 141:547-552(1988) [PubMed: 3385210] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[3]	"Mouse and human CD14 (myeloid cell-specific leucine-rich glycoprotein) primary structure deduced from cDNA clones." Setoguchi M., Nasu N., Yoshida S., Higuchi Y., Akizuki S., Yamamoto S. Biochim. Biophys. Acta 1008:213-222(1989) [PubMed: 2472171] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Macrophage.
[4]	"Monocyte antigen CD14 is a phospholipid anchored membrane protein." Simmons D.L., Tan S., Tenen D.G., Nicholson-Weller A., Seed B. Blood 73:284-289(1989) [PubMed: 2462937] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[5]	"Cloning and sequencing of human CD14 gene." Long J.Y., Xue Y.N., Sun L., Wang H.X. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Jin Zhan 25:377-378(1998) Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Promyelocytic leukemia.
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[7]	"Expression and secretion of CD14 in glial neoplasms of the brain." Deininger M.H., Meyermann R., Schluesener H.J. Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE OF 1-125. Tissue: Glioblastoma.
[8]	"Structural relationship between the soluble and membrane-bound forms of human monocyte surface glycoprotein CD14." Bazil V., Baudys M., Hilgert I., Stefanova I., Low M.G., Zbrozek J., Horejsi V. Mol. Immunol. 26:657-662(1989) [PubMed: 2779588] [Abstract] Cited for: PROTEIN SEQUENCE OF 362-367.
[9]	"Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry." Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E., Moore R.J., Smith R.D. J. Proteome Res. 4:2070-2080(2005) [PubMed: 16335952] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-151 AND ASN-282, MASS SPECTROMETRY. Tissue: Plasma.
+	Additional computationally mapped references.

Web resources

Wikipedia

CD14 entry

Cross-references

Sequence databases
EMBLGenBankDDBJ	X06882 Genomic DNA. Translation: CAA29999.1. X13334 mRNA. Translation: CAA31711.1. M86511 mRNA. Translation: AAA51930.1. AF097942 mRNA. Translation: AAC83816.1. BC010507 mRNA. Translation: AAH10507.1. AY044269 mRNA. Translation: AAL02401.1.
PIR	TDHUM4. A27637.
RefSeq	NP_000582.1. NP_001035110.1.
UniGene	Hs.163867
3D structure databases
SMR	P08571. Positions 24-332.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:1030N.
Proteomic databases
PeptideAtlas	P08571.
Genome annotation databases
Ensembl	ENSG00000170458. Homo sapiens. [Contig view]
GeneID	929.
KEGG	hsa:929.
Organism-specific databases
H-InvDB	HIX0005234.
HGNC	HGNC:1628. CD14.
HPA	HPA001887. HPA002035. HPA002127.
MIM	158120. gene.
PharmGKB	PA26188.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOGENOM	P08571.
HOVERGEN	P08571.
Enzyme and pathway databases
Reactome	REACT_6900. Signaling in Immune System.
Gene expression databases
ArrayExpress	P08571.
CleanEx	HS_CD14.
GermOnline	ENSG00000170458. Homo sapiens.
Family and domain databases
InterPro	IPR001611. LRR. IPR016337. Monocyte_diff_Ag_CD14. [Graphical view]
Pfam	PF00560. LRR_1. 3 hits. [Graphical view]
PIRSF	PIRSF002017. CD14. 1 hit.
PRINTS	PR00019. LEURICHRPT.
ProDom	P08571. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
SOURCE	Search...
ProtoNet	Search...

Entry information

Entry name

CD14_HUMAN

Accession

Primary (citable) accession number: P08571
Secondary accession number(s): Q96FR6, Q96L99, Q9UNS3

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	March 27, 2002
Last modified:	September 2, 2008
This is version 95 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)