Reviewed, UniProtKB/Swiss-Prot P08618 (UBIQ_DICDI)

Names and origin

Protein names

Recommended name:
    Ubiquitin

Gene names

Name:	ubqA
ORF Names:	DDB_0214921
AND
Name:	ubqB
ORF Names:	DDB_0214925
AND
Name:	ubqC
ORF Names:	DDB_0191355
AND
Name:	ubqD
Synonyms:	ubqE
ORF Names:	DDB_0214929
AND
Name:	ubqF
ORF Names:	DDB_0201651
AND
Name:	ubqG
Synonyms:	veg116
ORF Names:	DDB_0214920
AND
Name:	ubqH
ORF Names:	DDB_0238741
AND
Name:	ubqI
ORF Names:	DDB_0233885
AND
Name:	ubqJ
ORF Names:	DDB_0238742

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	76 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Protein modifier which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Attachment to proteins as a Lys-48-linked polymer usually leads to their degradation by proteasome. Attachment to proteins as a monomer or as an alternatively linked polymer does not lead to proteasomal degradation and may be required for numerous functions, including maintenance of chromatin structure, regulation of gene expression, stress response, ribosome biogenesis and DNA repair By similarity.
Subcellular location	Cytoplasm. Nucleus.
Miscellaneous	Ubiquitin is synthesized as a polyubiquitin precursor with exact head to tail repeats. Some ubiquitin genes contain a single copy of ubiquitin fused to a ribosomal protein. In D.discoideum there are 9 genes: ubqA: 5 copies of Ub and a final Asn; ubqB: 1 copy of Ub and ribosomal protein L40; ubqC: 1 copy of Ub and ribosomal protein S27A; ubqD: 3 copies of Ub and a final Leu; ubqF: 7 copies of Ub and a final Asn; ubqG: 5 copies of Ub and a final Leu; ubqH: 5 copies of Ub and a final Asn; ubqI: 4 copies of Ub and a final Asn; ubqJ: 4 copies of Ub and a final Asn.
Sequence similarities	Belongs to the ubiquitin family.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
PTM	Ubl conjugation
Technical term	Complete proteome
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 76	76	Ubiquitin
Amino acid modifications
	Cross-link	48		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity
	Cross-link	76		Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins) By similarity
Natural variations
	Natural variant	5	1	V → I in ubqC.
	Natural variant	19	1	G → C in ubqH 5th copy.
	Natural variant	28	1	A → T in ubqA 2nd, 3rd and 5th copies.

Sequences

	Sequence		Length	Mass (Da)
	P08618-1 [UniParc]. Last modified August 1, 1988. Version 1. Checksum: 6427383968EA8A84	FASTA	76	8,538
	10 20 30 40 50 60 MQIFVKTLTG KTITLEVEGS DNIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN 70 IQKESTLHLV LRLRGG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete cDNA sequence of a Dictyostelium ubiquitin with a carboxy-terminal tail and identification of the protein using an anti-peptide antibody." Mueller-Taubenberger A., Westphal M., Jaeger E., Noegel A., Gerisch G. FEBS Lett. 229:273-278(1988) [PubMed: 2831095] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (UBQB).
[2]	"Molecular organization of developmentally regulated Dictyostelium discoideum ubiquitin cDNAs." Ohmachi T., Giorda R., Shaw D.R., Ennis H.L. Biochemistry 28:5226-5231(1989) [PubMed: 2548604] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (UBQB).
[3]	"Structure of two developmentally regulated Dictyostelium discoideum ubiquitin genes." Giorda R., Ennis H.L. Mol. Cell. Biol. 7:2097-2103(1987) [PubMed: 3037345] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed: 12097910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (UBQC). Strain: AX4.
[5]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (UBQA; UBQB; UBQC; UBQD; UBQF; UBQG; UBQH; UBQI AND UBQJ). Strain: AX4.
[6]	"Transcript regulation and carboxyterminal extension of ubiquitin in Dictyostelium discoideum." Westphal M., Mueller-Taubenberg A., Noegel A., Gerisch G. FEBS Lett. 209:92-96(1986) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 13-76 (UBQB). Strain: AX2.
[7]	"Identification of novel centrosomal proteins in Dictyostelium discoideum by comparative proteomic approaches." Reinders Y., Schulz I., Graef R., Sickmann A. J. Proteome Res. 5:589-598(2006) [PubMed: 16512674] [Abstract] Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X07210 mRNA. Translation: CAA30183.1. Different termination. M23748 mRNA. Translation: AAA33262.1. Different termination. M23749 mRNA. Translation: AAA33263.1. Different termination. M23750 mRNA. Translation: AAA33264.1. Different termination. M23751 mRNA. Translation: AAA33265.1. Different termination. M23752 mRNA. Translation: AAA33266.1. Different termination. M23753 mRNA. Translation: AAA33267.1. Different termination. M23754 mRNA. Translation: AAA33268.1. Different termination. M19666 Genomic DNA. Translation: AAA33261.1. Different termination. M19491 Genomic DNA. Translation: AAA33269.1. Different termination. M19492 Genomic DNA. Translation: AAA33270.1. Different termination. AC115584 Genomic DNA. Translation: AAO50953.1. Different termination. AAFI02000005 Genomic DNA. Translation: EAL72079.1. Different termination. AAFI02000019 Genomic DNA. Translation: EAL68884.1. Different termination. AAFI02000032 Genomic DNA. Translation: EAL67635.1. Different termination. AAFI02000038 Genomic DNA. Translation: EAL67035.1. Different termination. AAFI02000047 Genomic DNA. Translation: EAL66269.1. Different termination. AAFI02000047 Genomic DNA. Translation: EAL66044.1. Different termination. AAFI02000092 Genomic DNA. Translation: EAL63951.1. Different termination. AAFI02000141 Genomic DNA. Translation: EAL62704.1. Different termination. AAFI02000186 Genomic DNA. Translation: EAL61494.1. Different termination. X04702 mRNA. Translation: CAA28408.1. Different termination.
PIR	A27806. A34080. B27806. B34080. C34080. D34080. UQDOR7. E34080. UQDOR. S00357.
RefSeq	XP_629924.1. XP_636210.1. XP_637465.1. XP_640098.1. XP_640258.1. XP_641020.1. XP_641560.1. XP_642815.1. XP_645986.1.
3D structure databases
HSSP	HSSP built from PDB template 1UBI based on UniProtKB P02248.
SMR	P08618. Positions 1-76.
ModBase	Search...
Genome annotation databases
GeneID	3386574. 3387788. 3389028. 3391330. 3391373. 3392117. 3392702. 3394100. 3397109.
KEGG	ddi:DDB_0191355. ddi:DDB_0201651. ddi:DDB_0214920. ddi:DDB_0214921. ddi:DDB_0214925. ddi:DDB_0214929. ddi:DDB_0233885. ddi:DDB_0238741. ddi:DDB_0238742.
Organism-specific databases
dictyBase	DDB0214921. ubqA. DDB0214925. ubqB. DDB0191355. ubqC. DDB0214929. ubqD. DDB0201651. ubqF. DDB0214920. ubqG. DDB0238741. ubqH. DDB0233885. ubqI. DDB0238742. ubqJ.
Family and domain databases
InterPro	IPR000626. Ubiquitin. [Graphical view]
Pfam	PF00240. ubiquitin. 1 hit. [Graphical view]
PRINTS	PR00348. UBIQUITIN.
SMART	SM00213. UBQ. 1 hit. [Graphical view]
PROSITE	PS00299. UBIQUITIN_1. 1 hit. PS50053. UBIQUITIN_2. 1 hit. [Graphical view]
ProDom	P08618. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

UBIQ_DICDI

Accession

Primary (citable) accession number: P08618
Secondary accession number(s): Q54HH5 , Q54L38, Q54SE1, Q54SP3, Q54UW7, Q54WJ3, Q550W7, Q55DZ5, Q86KQ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	August 1, 1988
Last modified:	September 2, 2008
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)