Skip Header

 
Contribute Send feedback

Reviewed, UniProtKB/Swiss-Prot P08648 (ITA5_HUMAN)

Last modified September 2, 2008. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein namesRecommended name:
    Integrin alpha-5
Alternative name(s):
    Fibronectin receptor subunit alpha
    Integrin alpha-F
    VLA-5
    CD49 antigen-like family member E
    CD_antigen=CD49e
Cleaved into the following 2 chains:
    1- Recommended name:
            Integrin alpha-5 heavy chain
    2- Recommended name:
            Integrin alpha-5 light chain
Gene names
Name: ITGA5
Synonyms: FNRA
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Hide | Top

Protein attributes

Sequence length1049 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

Hide | Top

General annotation (Comments)

Function

Integrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

Subunit structure

Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-5 associates with beta-1. Interacts with HPS5 and NISCH. Interacts with HIV-1 Tat.

Subcellular location

Membrane; Single-pass type I membrane protein.

Sequence similarities

Belongs to the integrin alpha chain family.

Contains 7 FG-GAP repeats.

Hide | Top

Ontologies

Keywords

   Biological processCell adhesion
Host-virus interaction
   Cellular componentMembrane
   DomainRepeat
Signal
Transmembrane
   LigandCalcium
   Molecular functionIntegrin
Receptor
   PTMCleavage on pair of basic residues
Glycoprotein
   Technical termDirect protein sequencing

Gene Ontology (GO)

   Biological processcell adhesion

Traceable author statement. Source: ProtInc

   Cellular componentintegrin complex

Traceable author statement. Source: ProtInc

ruffle

Traceable author statement. Source: HGNC

Complete GO annotation...

Hide | Top

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical view

Molecule processing

Signal peptide1 – 4141
Chain42 – 10491008Integrin alpha-5
Chain42 – 894853Integrin alpha-5 heavy chain
Chain895 – 1049155Integrin alpha-5 light chain

Regions

Topological domain42 – 995954Extracellular Potential
Transmembrane996 – 102126 Potential
Topological domain1022 – 104928Cytoplasmic Potential
Repeat57 – 11963FG-GAP 1
Repeat131 – 19868FG-GAP 2
Repeat199 – 25658FG-GAP 3
Repeat269 – 32254FG-GAP 4
Repeat323 – 38967FG-GAP 5
Repeat390 – 44960FG-GAP 6
Repeat453 – 50553FG-GAP 7
Calcium binding334 – 3429 Potential
Calcium binding401 – 4099 Potential
Calcium binding465 – 4739 Potential
Region1021 – 10288Interaction with HPS5
Motif1024 – 10285GFFKR motif

Amino acid modifications

Glycosylation841N-linked (GlcNAc...) Potential
Glycosylation1821N-linked (GlcNAc...) Potential
Glycosylation2971N-linked (GlcNAc...) Potential
Glycosylation3071N-linked (GlcNAc...) Potential
Glycosylation3161N-linked (GlcNAc...) Potential
Glycosylation5241N-linked (GlcNAc...) Potential
Glycosylation5301N-linked (GlcNAc...) Potential
Glycosylation5931N-linked (GlcNAc...) Potential
Glycosylation6091N-linked (GlcNAc...) Potential
Glycosylation6751N-linked (GlcNAc...) Potential
Glycosylation7121N-linked (GlcNAc...) Potential
Glycosylation7241N-linked (GlcNAc...) Potential
Glycosylation7731N-linked (GlcNAc...) Potential
Glycosylation8681N-linked (GlcNAc...) Potential
Disulfide bond99 ↔ 108
Disulfide bond156 ↔ 176
Disulfide bond192 ↔ 205
Disulfide bond513 ↔ 522 By similarity
Disulfide bond528 ↔ 584 By similarity
Disulfide bond645 ↔ 651
Disulfide bond718 ↔ 731
Disulfide bond869 ↔ 921Interchain (between heavy and light chains)
Disulfide bond911 ↔ 916

Experimental info

Sequence conflict261L → V Ref.1 Ref.3
Sequence conflict331L → V Ref.1 Ref.3

Hide | Top

Sequences

Sequence LengthMass (Da)Tools
P08648-1 [UniParc].

Last modified October 10, 2002. Version 2.
Checksum: 6B4D558D4F739CBA

FASTA1,049114,536
        10         20         30         40         50         60 
MGSRTPESPL HAVQLRWGPR RRPPLLPLLL LLLPPPPRVG GFNLDAEAPA VLSGPPGSFF 

        70         80         90        100        110        120 
GFSVEFYRPG TDGVSVLVGA PKANTSQPGV LQGGAVYLCP WGASPTQCTP IEFDSKGSRL 

       130        140        150        160        170        180 
LESSLSSSEG EEPVEYKSLQ WFGATVRAHG SSILACAPLY SWRTEKEPLS DPVGTCYLST 

       190        200        210        220        230        240 
DNFTRILEYA PCRSDFSWAA GQGYCQGGFS AEFTKTGRVV LGGPGSYFWQ GQILSATQEQ 

       250        260        270        280        290        300 
IAESYYPEYL INLVQGQLQT RQASSIYDDS YLGYSVAVGE FSGDDTEDFV AGVPKGNLTY 

       310        320        330        340        350        360 
GYVTILNGSD IRSLYNFSGE QMASYFGYAV AATDVNGDGL DDLLVGAPLL MDRTPDGRPQ 

       370        380        390        400        410        420 
EVGRVYVYLQ HPAGIEPTPT LTLTGHDEFG RFGSSLTPLG DLDQDGYNDV AIGAPFGGET 

       430        440        450        460        470        480 
QQGVVFVFPG GPGGLGSKPS QVLQPLWAAS HTPDFFGSAL RGGRDLDGNG YPDLIVGSFG 

       490        500        510        520        530        540 
VDKAVVYRGR PIVSASASLT IFPAMFNPEE RSCSLEGNPV ACINLSFCLN ASGKHVADSI 

       550        560        570        580        590        600 
GFTVELQLDW QKQKGGVRRA LFLASRQATL TQTLLIQNGA REDCREMKIY LRNESEFRDK 

       610        620        630        640        650        660 
LSPIHIALNF SLDPQAPVDS HGLRPALHYQ SKSRIEDKAQ ILLDCGEDNI CVPDLQLEVF 

       670        680        690        700        710        720 
GEQNHVYLGD KNALNLTFHA QNVGEGGAYE AELRVTAPPE AEYSGLVRHP GNFSSLSCDY 

       730        740        750        760        770        780 
FAVNQSRLLV CDLGNPMKAG ASLWGGLRFT VPHLRDTKKT IQFDFQILSK NLNNSQSDVV 

       790        800        810        820        830        840 
SFRLSVEAQA QVTLNGVSKP EAVLFPVSDW HPRDQPQKEE DLGPAVHHVY ELINQGPSSI 

       850        860        870        880        890        900 
SQGVLELSCP QALEGQQLLY VTRVTGLNCT TNHPINPKGL ELDPEGSLHH QQKREAPSRS 

       910        920        930        940        950        960 
SASSGPQILK CPEAECFRLR CELGPLHQQE SQSLQLHFRV WAKTFLQREH QPFSLQCEAV 

       970        980        990       1000       1010       1020 
YKALKMPYRI LPRQLPQKER QVATAVQWTK AEGSYGVPLW IIILAILFGL LLLGLLIYIL 

      1030       1040 
YKLGFFKRSL PYGTAMEKAQ LKPPATSDA

« Hide

Hide | Top

References

« Hide 'large scale' references
[1]"Amino acid sequence of the human fibronectin receptor."
Argraves W.S., Suzuki S., Arai H., Thompson K., Pierschbacher M.D., Ruoslahti E.
J. Cell Biol. 105:1183-1190(1987) [PubMed: 2958481] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Placenta.
[3]"The alpha 5 beta 1 fibronectin receptor. Characterization of the alpha 5 gene promoter."
Birkenmeier T.M., McQuillan J.J., Boedeker E.D., Argraves W.S., Ruoslahti E., Dean D.C.
J. Biol. Chem. 266:20544-20549(1991) [PubMed: 1834647] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-73.
[4]"Comparison of cDNA-derived protein sequences of the human fibronectin and vitronectin receptor alpha-subunits and platelet glycoprotein IIb."
Fitzgerald L.A., Poncz M., Steiner B., Rall S.C., Bennett J.S., Phillips D.R.
Biochemistry 26:8158-8165(1987) [PubMed: 2450560] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 16-1049.
[5]"cDNA sequences from the alpha subunit of the fibronectin receptor predict a transmembrane domain and a short cytoplasmic peptide."
Argraves W.S., Pytela R., Suzuki S., Millan J.L., Pierschbacher M.D., Ruoslahti E.
J. Biol. Chem. 261:12922-12924(1986) [PubMed: 2944883] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 821-1049.
[6]"The very late antigen family of heterodimers is part of a superfamily of molecules involved in adhesion and embryogenesis."
Takada Y., Strominger J.L., Hemler M.E.
Proc. Natl. Acad. Sci. U.S.A. 84:3239-3243(1987) [PubMed: 3033641] [Abstract]
Cited for: PROTEIN SEQUENCE OF 42-55.
[7]"The Tat protein of human immunodeficiency virus type-1 promotes vascular cell growth and locomotion by engaging the alpha5beta1 and alphavbeta3 integrins and by mobilizing sequestered basic fibroblast growth factor."
Barillari G., Sgadari C., Fiorelli V., Samaniego F., Colombini S., Manzari V., Modesti A., Nair B.C., Cafaro A., Stuerzl M., Ensoli B.
Blood 94:663-672(1999) [PubMed: 10397733] [Abstract]
Cited for: INTERACTION WITH HIV-1 TAT.
[8]"Insulin receptor substrate 4 associates with the protein IRAS."
Sano H., Liu S.C.H., Lane W.S., Piletz J.E., Lienhard G.E.
J. Biol. Chem. 277:19439-19447(2002) [PubMed: 11912194] [Abstract]
Cited for: INTERACTION WITH NISCH.
[9]"Mass spectrometric based mapping of the disulfide bonding patterns of integrin alpha chains."
Krokhin O.V., Cheng K., Sousa S.L., Ens W., Standing K.G., Wilkins J.A.
Biochemistry 42:12950-12959(2003) [PubMed: 14596610] [Abstract]
Cited for: DISULFIDE BONDS.
+Additional computationally mapped references.

Hide | Top

Cross-references

Sequence databases

X06256 mRNA. Translation: CAA29601.1.
BC008786 mRNA. Translation: AAH08786.1.
M13918 mRNA. Translation: AAA52467.1. Sequence problems.
PIRA27079.
RefSeqNP_002196.2.
UniGeneHs.505654

3D structure databases

HSSPHSSP built from PDB template 1L5G based on UniProtKB P06756.
ModBaseSearch...

Protein-protein interaction databases

IntActP08648.

PTM databases

GlycoSuiteDBP08648.

Proteomic databases

PeptideAtlasP08648.

Genome annotation databases

EnsemblENSG00000161638. Homo sapiens. [Contig view]
GeneID3678.
KEGGhsa:3678.

Organism-specific databases

H-InvDBHIX0010697.
HGNCHGNC:6141. ITGA5.
HPACAB009008.
HPA002642.
MIM135620. gene.
PharmGKBPA29941.
GenAtlasSearch...
GeneCardsSearch...

Phylogenomic databases

HOGENOMP08648.
HOVERGENP08648.

Gene expression databases

ArrayExpressP08648.
CleanExHS_ITGA5.
GermOnlineENSG00000161638. Homo sapiens.

Family and domain databases

InterProIPR013517. FG-GAP.
IPR013519. Int_alpha_beta-p.
IPR000413. Integrin_alpha.
IPR013649. Integrin_alpha-2.
IPR013513. Integrin_alpha_C.
[Graphical view]
PfamPF01839. FG-GAP. 3 hits.
PF00357. Integrin_alpha. 1 hit.
PF08441. Integrin_alpha2. 1 hit.
[Graphical view]
PRINTSPR01185. INTEGRINA.
SMARTSM00191. Int_alpha. 5 hits.
[Graphical view]
PROSITEPS00242. INTEGRIN_ALPHA. 1 hit.
[Graphical view]
ProDomP08648.
[Graphical view] [Entries sharing at least one domain]
BLOCKSSearch...

Other Resources

SOURCESearch...
ProtoNetSearch...

Hide | Top

Entry information

Entry nameITA5_HUMAN
AccessionPrimary (citable) accession number: P08648
Secondary accession number(s): Q96HA5
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: October 10, 2002
Last modified: September 2, 2008
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Hide | Top

Relevant documents

Human cell differentiation molecules

CD nomenclature of surface proteins of human leucocytes and list of entries

Human chromosome 12

Human chromosome 12: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

UniProtKB secondary accession numbers

Index of UniProtKB secondary accession numbers

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents