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Reviewed, UniProtKB/Swiss-Prot P08659 (LUCI_PHOPY)

Last modified November 25, 2008. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Luciferin 4-monooxygenase
      Short name=Luciferase
    EC=1.13.12.7
OrganismPhotinus pyralis (North American firefly)
Taxonomic identifier7054 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Coleoptera › Polyphaga › Elateriformia › Elateroidea › Lampyridae › Lampyrinae › Photinus

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Protein attributes

Sequence length550 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Produces green light with a wavelength of 562 nm.

Catalytic activity

Photinus luciferin + O(2) + ATP = oxidized Photinus luciferin + CO(2) + AMP + diphosphate + light.

Cofactor

Magnesium.

Subcellular location

Peroxisome. Ref.3

Sequence similarities

Belongs to the ATP-dependent AMP-binding enzyme family.

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Ontologies

Keywords

   Biological processLuminescence
   Cellular componentPeroxisome
   LigandMagnesium
Metal-binding
   Molecular functionMonooxygenase
Oxidoreductase
Photoprotein
   Technical term3D-structure

Gene Ontology (GO)

   Biological processbioluminescence

Inferred from electronic annotation. Source: UniProtKB-KW

oxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentperoxisome Ref.3

Inferred from direct assay. Source: UniProtKB

   Molecular functionPhotinus-luciferin 4-monooxygenase (ATP-hydrolyzing) activity

Inferred from electronic annotation. Source: EC

magnesium ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 550550Luciferin 4-monooxygenase
PRO_0000193147

Regions

Motif548 – 5503Microbody targeting signal

Experimental info

Sequence conflict3611A → G in CAA59281. Ref.2

Secondary structure

1 .................................................................................................. 550
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
P08659-1 [UniParc].

Last modified January 1, 1988. Version 1.
Checksum: E380FCE9D56ACCDE

FASTA55060,745
        10         20         30         40         50         60 
MEDAKNIKKG PAPFYPLEDG TAGEQLHKAM KRYALVPGTI AFTDAHIEVN ITYAEYFEMS 

        70         80         90        100        110        120 
VRLAEAMKRY GLNTNHRIVV CSENSLQFFM PVLGALFIGV AVAPANDIYN ERELLNSMNI 

       130        140        150        160        170        180 
SQPTVVFVSK KGLQKILNVQ KKLPIIQKII IMDSKTDYQG FQSMYTFVTS HLPPGFNEYD 

       190        200        210        220        230        240 
FVPESFDRDK TIALIMNSSG STGLPKGVAL PHRTACVRFS HARDPIFGNQ IIPDTAILSV 

       250        260        270        280        290        300 
VPFHHGFGMF TTLGYLICGF RVVLMYRFEE ELFLRSLQDY KIQSALLVPT LFSFFAKSTL 

       310        320        330        340        350        360 
IDKYDLSNLH EIASGGAPLS KEVGEAVAKR FHLPGIRQGY GLTETTSAIL ITPEGDDKPG 

       370        380        390        400        410        420 
AVGKVVPFFE AKVVDLDTGK TLGVNQRGEL CVRGPMIMSG YVNNPEATNA LIDKDGWLHS 

       430        440        450        460        470        480 
GDIAYWDEDE HFFIVDRLKS LIKYKGYQVA PAELESILLQ HPNIFDAGVA GLPDDDAGEL 

       490        500        510        520        530        540 
PAAVVVLEHG KTMTEKEIVD YVASQVTTAK KLRGGVVFVD EVPKGLTGKL DARKIREILI 

       550 
KAKKGGKSKL

« Hide

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References

[1]"Firefly luciferase gene: structure and expression in mammalian cells."
de Wet J.R., Wood K.V., Deluca M., Helinski D.R., Subramani S.
Mol. Cell. Biol. 7:725-737(1987) [PubMed: 3821727] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Construction and utilization of an Autographa californica nuclear polyhedrosis virus vector with a unique cloning site: expression of genes amplified by the polymerase chain reaction."
Croizier G.M.J.
Submitted (JUL-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Firefly luciferase is targeted to peroxisomes in mammalian cells."
Keller G.-A., Gould S., de Luca M., Subramani S.
Proc. Natl. Acad. Sci. U.S.A. 84:3264-3268(1987) [PubMed: 3554235] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[4]"Crystal structure of firefly luciferase throws light on a superfamily of adenylate-forming enzymes."
Conti E., Franks N.P., Brick P.
Structure 4:287-298(1996) [PubMed: 8805533] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
[5]"Structural basis for the inhibition of firefly luciferase by a general anesthetic."
Franks N.P., Jenkins A., Conti E., Lieb W.R., Brick P.
Biophys. J. 75:2205-2211(1998) [PubMed: 9788915] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).

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Web resources

Wikipedia

Firefly luciferase entry

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Cross-references

Sequence databases

M15077 Genomic DNA. Translation: AAA29795.1.
X84846 Genomic DNA. Translation: CAA59281.1.
X84848 Genomic DNA. Translation: CAA59283.1.
U03687 Unassigned DNA. Translation: AAA03561.1.
U89934 Genomic DNA. Translation: AAB64396.1.
U89935 Genomic DNA. Translation: AAB64399.1.
PIRA26772.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1BA3X-ray2.20A1-550[»]
1LCIX-ray2.00A1-550[»]
ModBaseSearch...

Family and domain databases

InterProIPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamPF00501. AMP-binding. 1 hit.
[Graphical view]
PRINTSPR00154. AMPBINDING.
PROSITEPS00455. AMP_BINDING. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

LinkHubP08659.

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Entry information

Entry nameLUCI_PHOPY
AccessionPrimary (citable) accession number: P08659
Secondary accession number(s): Q27755
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: November 25, 2008
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

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Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents