Reviewed, UniProtKB/Swiss-Prot P08704 (CDGT_KLEOX)

Names and origin

Protein names

Recommended name:
    Cyclomaltodextrin glucanotransferase
    EC=2.4.1.19
Alternative name(s):
    Cyclodextrin-glycosyltransferase
      Short name=CGTase

Gene names

Name:

cgt

Organism

Klebsiella oxytoca

Taxonomic identifier

571 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length	655 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Cyclizes part of a (1->4)-alpha-D-glucan chain by formation of a (1->4)-alpha-D-glucosidic bond.
Cofactor	Binds 2 calcium ions per subunit By similarity.
Subunit structure	Monomer.
Domain	May consist of two protein domains: the one in the N-terminal side cleaves the alpha-1,4-glucosidic bond in starch, and the other in the C-terminal side catalyzes other activities, including the reconstitution of an alpha-1,4-glucosidic linkage for cyclizing the maltooligosaccharide produced.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. Contains 1 CBM20 (carbohydrate binding type-20) domain.

Ontologies

Keywords
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Glycosyltransferase Transferase
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW carbohydrate binding Inferred from electronic annotation. Source: InterPro cyclomaltodextrin glucanotransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 30	30
	Chain	31 – 655	625	Cyclomaltodextrin glucanotransferase		PRO_0000001443
Regions
	Domain	554 – 655	102	CBM20
Sites
	Active site	253	1	Nucleophile By similarity
	Active site	287	1	Proton donor By similarity
	Active site	363	1	By similarity
	Metal binding	55	1	Calcium 2 By similarity
	Metal binding	60	1	Calcium 2 By similarity
	Metal binding	61	1	Calcium 2 By similarity
	Metal binding	79	1	Calcium 2; via carbonyl oxygen By similarity
	Metal binding	81	1	Calcium 2 By similarity
	Metal binding	164	1	Calcium 1 By similarity
	Metal binding	223	1	Calcium 1 By similarity
	Metal binding	257	1	Calcium 1; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	P08704-1 [UniParc]. Last modified January 1, 1988. Version 1. Checksum: DB8F26332BED26A7	FASTA	655	73,025
	10 20 30 40 50 60 MKRNRFFNTS AAIAISIALN TFFCSMQTIA AEPEETYLDF RKETIYFLFL DRFSDGDPSN 70 80 90 100 110 120 NAGFNSATYD PNNLKKYTGG DLRGLINKLP YLKSLGVTSI WITPPIDNVN NTDAAGNTGY 130 140 150 160 170 180 HGYWGRDYFR IDEHFGNLDD FKELTSLMHS PDYNMKLVLD YAPNHSNAND ENEFGALYRD 190 200 210 220 230 240 GVFITDYPTN VAANTGWYHH NGGVTNWNDF FQVKNHNLFN LSDLNQSNTD VYQYLLDGSK 250 260 270 280 290 300 FWIDAGVDAI RIDAIKHMDK SFIQKWTSDI YDYSKSIGRE GFFFFGEWFG ASANTTTGVD 310 320 330 340 350 360 GNAIDYANTS GSALLDFGFR DTLERVLVGR SGNTMKTLNS YLIKRQTVFT SDDWQVVFMD 370 380 390 400 410 420 NHDMARIGTA LRSNATTFGP GNNETGGSQS EAFAQKRIDL GLVATMTVRG IPAIYYGTEH 430 440 450 460 470 480 YAANFTSNSF GQVGSDPYNR EKMPGFDTES EAFSIIKTLG DLRKSSPAIQ NGTYTELWVN 490 500 510 520 530 540 DDILVFERRS GNDIVIVALN RGEANTINVK NIAVPNGVYP SLIGNNSVSV ANKRTTLTLM 550 560 570 580 590 600 QNEAVVIRSQ SDDAENPTVQ SINFTCNNGY TISGQSVYII GNIPQLGGWD LTKAVKISPT 610 620 630 640 650 QYPQWSASLE LPSDLNVEWK CVKRNETNPT ANVEWQSGAN NQFNSNDTQT TNGSF

References

[1]	"Cyclodextrin-glycosyltransferase from Klebsiella pneumoniae M5a1: cloning, nucleotide sequence and expression." Binder F., Huber O., Boeck A. Gene 47:269-277(1986) [PubMed: 2951300] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: M5a1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M15264 Genomic DNA. Translation: AAA25059.1.
PIR	ALKBG. A29023.
3D structure databases
HSSP	HSSP built from PDB template 1QHP based on UniProtKB P19531.
ModBase	Search...
Family and domain databases
InterPro	IPR006048. A-amylase_b_C. IPR006046. Glyco_hydro_13. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat. IPR006589. Glyco_hydro_13_sub_cat. IPR002044. Glyco_hydro_carb-bd. IPR013781. Glyco_hydro_sub_cat. IPR013783. Ig-like_fold. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF00686. CBM_20. 1 hit. [Graphical view]
PRINTS	PR00110. ALPHAAMYLASE.
ProDom	PD001568. Glyco_hydro_CBD. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00642. Aamy. 1 hit. SM00632. Aamy_C. 1 hit. [Graphical view]
PROSITE	PS51166. CBM20. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CDGT_KLEOX

Accession

Primary (citable) accession number: P08704

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 1, 1988
Last modified:	November 25, 2008
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)