Reviewed, UniProtKB/Swiss-Prot P08908 (5HT1A_HUMAN)

Names and origin

Protein names

Recommended name:
    5-hydroxytryptamine receptor 1A
      Short name(s)=5-HT-1A, 5-HT1A
Alternative name(s):
    Serotonin receptor 1A
    G-21

Gene names

Name:	HTR1A
Synonyms:	ADRB2RL1, ADRBRL1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	422 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein Ubl conjugation
Gene Ontology (GO)
Biological process	G-protein coupled receptor protein signaling pathway Traceable author statement. Source: ProtInc behavior Traceable author statement. Source: ProtInc positive regulation of cell proliferation Traceable author statement. Source: ProtInc
Cellular component	integral to plasma membrane Ref.8 Traceable author statement. Source: ProtInc
Molecular function	serotonin receptor activity Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 422	422	5-hydroxytryptamine receptor 1A
Regions
	Topological domain	1 – 36	36	Extracellular Potential
	Transmembrane	37 – 62	26	1 Potential
	Topological domain	63 – 73	11	Cytoplasmic Potential
	Transmembrane	74 – 98	25	2 Potential
	Topological domain	99 – 109	11	Extracellular Potential
	Transmembrane	110 – 132	23	3 Potential
	Topological domain	133 – 152	20	Cytoplasmic Potential
	Transmembrane	153 – 178	26	4 Potential
	Topological domain	179 – 191	13	Extracellular Potential
	Transmembrane	192 – 217	26	5 Potential
	Topological domain	218 – 345	128	Cytoplasmic Potential
	Transmembrane	346 – 367	22	6 Potential
	Topological domain	368 – 378	11	Extracellular Potential
	Transmembrane	379 – 403	25	7 Potential
	Topological domain	404 – 422	19	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	10	1	N-linked (GlcNAc...) Potential
	Glycosylation	11	1	N-linked (GlcNAc...) Potential
	Glycosylation	24	1	N-linked (GlcNAc...) Potential
	Disulfide bond	109 ↔ 187		By similarity
	Cross-link	334		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
Natural variations
	Natural variant	16	1	P → L: dbSNP rs1800041.
	Natural variant	22	1	G → S: dbSNP rs1799920.
	Natural variant	28	1	I → V: dbSNP rs1799921.
	Natural variant	184	1	P → L: dbSNP rs1800043.
	Natural variant	220	1	R → L: dbSNP rs1800044.
	Natural variant	273	1	G → D: dbSNP rs1800042.
Experimental info
	Sequence conflict	152 – 154	3	RAA → PR in AAA36440 and CAA31908. Ref.1
	Sequence conflict	172	1	M → I in AAA36440 and CAA31908. Ref.1
	Sequence conflict	200 – 202	3	TFG → RPR Ref.7
	Sequence conflict	228	1	K → R Ref.7
	Sequence conflict	244	1	A → AA Ref.7
	Sequence conflict	355	1	I → T Ref.7
	Sequence conflict	363 – 365	3	IVA → MRP Ref.7
	Sequence conflict	418	1	K → N in AAA36440 and CAA31908. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P08908-1 [UniParc]. Last modified April 1, 1993. Version 3. Checksum: 762664FCF62CFD8F	FASTA	422	46,107
	10 20 30 40 50 60 MDVLSPGQGN NTTSPPAPFE TGGNTTGISD VTVSYQVITS LLLGTLIFCA VLGNACVVAA 70 80 90 100 110 120 IALERSLQNV ANYLIGSLAV TDLMVSVLVL PMAALYQVLN KWTLGQVTCD LFIALDVLCC 130 140 150 160 170 180 TSSILHLCAI ALDRYWAITD PIDYVNKRTP RRAAALISLT WLIGFLISIP PMLGWRTPED 190 200 210 220 230 240 RSDPDACTIS KDHGYTIYST FGAFYIPLLL MLVLYGRIFR AARFRIRKTV KKVEKTGADT 250 260 270 280 290 300 RHGASPAPQP KKSVNGESGS RNWRLGVESK AGGALCANGA VRQGDDGAAL EVIEVHRVGN 310 320 330 340 350 360 SKEHLPLPSE AGPTPCAPAS FERKNERNAE AKRKMALARE RKTVKTLGII MGTFILCWLP 370 380 390 400 410 420 FFIVALVLPF CESSCHMPTL LGAIINWLGY SNSLLNPVIY AYFNKDFQNA FKKIIKCKFC RQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"An intronless gene encoding a potential member of the family of receptors coupled to guanine nucleotide regulatory proteins." Kobilka B.K., Frielle T., Collins S., Yang-Feng T.L., Kobilka T.S., Francke U., Lefkowitz R.J., Caron M.G. Nature 329:75-79(1987) [PubMed: 3041227] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Saltzman A.G., Morse B., Felder S. Submitted (FEB-1991) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Silver project." Kitano T., Kobayakawa H., Saitou N. Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Puhl H.L. III, Ikeda S.R., Aronstam R.S. Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"A polymerase chain reaction mediated by a single primer: cloning of genomic sequences adjacent to a serotonin receptor protein coding region." Parks C.L., Chang L.S., Shenk T. Nucleic Acids Res. 19:7155-7160(1991) [PubMed: 1766875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 1-9.
[7]	"Expression of 5HT1a receptors on activated human T cells. Regulation of cyclic AMP levels and T cell proliferation by 5-hydroxytryptamine." Aune T.M., McGrath K.M., Sarr T., Bombara M.P., Kelley K.A. J. Immunol. 151:1175-1183(1993) [PubMed: 8393041] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 200-365.
[8]	"The genomic clone G-21 which resembles a beta-adrenergic receptor sequence encodes the 5-HT1A receptor." Fargin A., Raymond J.R., Lohse M.L., Kobilka B.K., Caron M.G., Lefkowitz R.J. Nature 335:358-360(1988) [PubMed: 3138543] [Abstract] Cited for: FUNCTION.
[9]	"The proteomic reactor facilitates the analysis of affinity-purified proteins by mass spectrometry: application for identifying ubiquitinated proteins in human cells." Vasilescu J., Zweitzig D.R., Denis N.J., Smith J.C., Ethier M., Haines D.S., Figeys D. J. Proteome Res. 6:298-305(2007) [PubMed: 17203973] [Abstract] Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-334, MASS SPECTROMETRY. Tissue: Lung adenocarcinoma.
[10]	"Two naturally occurring amino acid substitutions in the human 5-HT1A receptor: glycine 22 to serine 22 and isoleucine 28 to valine 28." Nakhai B., Nielsen D.A., Linnoila M., Goldman D. Biochem. Biophys. Res. Commun. 210:530-536(1995) [PubMed: 7755630] [Abstract] Cited for: VARIANTS SER-22 AND VAL-28.
[11]	"Novel mutations in the promoter and coding region of the human 5-HT1A receptor gene and association analysis in schizophrenia." Kawanishi Y., Harada S., Tachikawa H., Okubo T., Shiraishi H. Am. J. Med. Genet. 81:434-439(1998) [PubMed: 9754630] [Abstract] Cited for: VARIANTS LEU-16 AND ASP-273.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M28269 Genomic DNA. Translation: AAA36440.1. X13556 Genomic DNA. Translation: CAA31908.1. X57829 Genomic DNA. Translation: CAA40962.1. M83181 Genomic DNA. Translation: AAA66493.1. AB041403 Genomic DNA. Translation: BAA94488.1. BC069159 mRNA. Translation: AAH69159.1. AF498978 mRNA. Translation: AAM21125.1. Z11168 Genomic DNA. Translation: CAA77560.1.
PIR	I38209.
RefSeq	NP_000515.2.
UniGene	Hs.247940
3D structure databases
HSSP	HSSP built from PDB template 1HLL based on UniProtKB P08913.
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
Genome annotation databases
Ensembl	ENSG00000178394. Homo sapiens. [Contig view]
GeneID	3350.
KEGG	hsa:3350.
Organism-specific databases
H-InvDB	HIX0032004.
HGNC	HGNC:5286. HTR1A.
HPA	HPA018073.
MIM	109760. gene.
PharmGKB	PA192.
GenAtlas	Search...
GeneCards	Search...
GeneLynx	Search...
Phylogenomic databases
HOGENOM	P08908.
HOVERGEN	P08908.
Gene expression databases
ArrayExpress	P08908.
CleanEx	HS_HTR1A.
GermOnline	ENSG00000178394. Homo sapiens.
Family and domain databases
InterPro	IPR000610. 5HT1A_rcpt. IPR002231. 5HT_rcpt. IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_supfam. [Graphical view]
PANTHER	PTHR19266:SF64. 5HT1A_rcpt. 1 hit.
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00512. 5HT1ARECEPTR. PR01101. 5HTRECEPTOR. PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProDom	P08908. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
DrugBank	DB00866. Alprenolol. DB01238. Aripiprazole. DB00490. Buspirone. DB00363. Clozapine. DB00216. Eletriptan. DB01049. Ergoloid mesylate. DB00176. Fluvoxamine. DB00589. Lisuride. DB00247. Methysergide. DB00370. Mirtazapine. DB00960. Pindolol. DB00571. Propranolol. DB01224. Quetiapine. DB01104. Sertraline. DB01079. Tegaserod. DB00656. Trazodone. DB00285. Venlafaxine. DB00246. Ziprasidone.
SOURCE	Search...
ProtoNet	Search...

Entry information

Entry name

5HT1A_HUMAN

Accession

Primary (citable) accession number: P08908
Secondary accession number(s): Q6LAE7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	April 1, 1993
Last modified:	July 22, 2008
This is version 92 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)