Reviewed, UniProtKB/Swiss-Prot P08912 (ACM5_HUMAN)

Names and origin

Protein names

Recommended name:
    Muscarinic acetylcholine receptor M5

Gene names

Name:

CHRM5

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	532 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.
Subcellular location	Cell membrane; Multi-pass membrane protein. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell junction Cell membrane Membrane Postsynaptic cell membrane Synapse
Domain	Transmembrane
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein Phosphoprotein
Gene Ontology (GO)
Biological process	acetylcholine receptor signaling, muscarinic pathway Ref.1 Traceable author statement. Source: ProtInc cell proliferation Traceable author statement. Source: ProtInc muscarinic acetylcholine receptor, adenylate cyclase inhibiting pathway Traceable author statement. Source: ProtInc
Cellular component	integral to plasma membrane Ref.1 Traceable author statement. Source: ProtInc
Molecular function	muscarinic acetylcholine receptor activity Ref.1 Traceable author statement. Source: ProtInc phosphoinositide phospholipase C activity Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 532	532	Muscarinic acetylcholine receptor M5
Regions
	Topological domain	1 – 29	29	Extracellular Potential
	Transmembrane	30 – 53	24	1 Potential
	Topological domain	54 – 66	13	Cytoplasmic Potential
	Transmembrane	67 – 87	21	2 Potential
	Topological domain	88 – 104	17	Extracellular Potential
	Transmembrane	105 – 126	22	3 Potential
	Topological domain	127 – 146	20	Cytoplasmic Potential
	Transmembrane	147 – 169	23	4 Potential
	Topological domain	170 – 191	22	Extracellular Potential
	Transmembrane	192 – 214	23	5 Potential
	Topological domain	215 – 443	229	Cytoplasmic Potential
	Transmembrane	444 – 464	21	6 Potential
	Topological domain	465 – 478	14	Extracellular Potential
	Transmembrane	479 – 498	20	7 Potential
	Topological domain	499 – 532	34	Cytoplasmic Potential
Amino acid modifications
	Modified residue	501	1	Phosphothreonine Potential
	Modified residue	505	1	Phosphothreonine Potential
	Glycosylation	8	1	N-linked (GlcNAc...) Potential
	Disulfide bond	103 ↔ 183		By similarity
Experimental info
	Sequence conflict	526	1	Q → P Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P08912-1 [UniParc]. Last modified June 1, 1994. Version 2. Checksum: EE846186F645DD9B	FASTA	532	60,074
	10 20 30 40 50 60 MEGDSYHNAT TVNGTPVNHQ PLERHRLWEV ITIAAVTAVV SLITIVGNVL VMISFKVNSQ 70 80 90 100 110 120 LKTVNNYYLL SLACADLIIG IFSMNLYTTY ILMGRWALGS LACDLWLALD YVASNASVMN 130 140 150 160 170 180 LLVISFDRYF SITRPLTYRA KRTPKRAGIM IGLAWLISFI LWAPAILCWQ YLVGKRTVPL 190 200 210 220 230 240 DECQIQFLSE PTITFGTAIA AFYIPVSVMT ILYCRIYRET EKRTKDLADL QGSDSVTKAE 250 260 270 280 290 300 KRKPAHRALF RSCLRCPRPT LAQRERNQAS WSSSRRSTST TGKPSQATGP SANWAKAEQL 310 320 330 340 350 360 TTCSSYPSSE DEDKPATDPV LQVVYKSQGK ESPGEEFSAE ETEETFVKAE TEKSDYDTPN 370 380 390 400 410 420 YLLSPAAAHR PKSQKCVAYK FRLVVKADGN QETNNGCHKV KIMPCPFPVA KEPSTKGLNP 430 440 450 460 470 480 NPSHQMTKRK RVVLVKERKA AQTLSAILLA FIITWTPYNI MVLVSTFCDK CVPVTLWHLG 490 500 510 520 530 YWLCYVNSTV NPICYALCNR TFRKTFKMLL LCRWKKKKVE EKLYWQGNSK LP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and expression of the human and rat m5 muscarinic acetylcholine receptor genes." Bonner T.I., Young A.C., Brann M.R., Buckley N.J. Neuron 1:403-410(1988) [PubMed: 3272174] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	Rae J.L., Shepard A.R. Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Tissue: Lens epithelium.
[3]	"Human muscarinic acetylcholine receptor 5 is localized on 15q13.1 and its expression in SH-SY5Y cells." Wang H., Yeomans J. Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Tissue: Brain.
[4]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Puhl H.L. III, Ikeda S.R., Aronstam R.S. Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[5]	"Cloning cholinergic receptors in human keratinocytes." Arredondo J., Grando S.A. Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE OF 1-526. Tissue: Keratinocyte.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M80333 Genomic DNA. Translation: AAA51569.1. AF026263 mRNA. Translation: AAB95158.1. AB084282 mRNA. Translation: BAB91222.1. AF498919 mRNA. Translation: AAM18942.1. AF385591 mRNA. Translation: AAK68116.1.
PIR	JT0530.
RefSeq	NP_036257.1.
UniGene	Hs.584747
3D structure databases
HSSP	HSSP built from PDB template 1HLL based on UniProtKB P08913.
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
Genome annotation databases
Ensembl	ENSG00000184984. Homo sapiens. [Contig view]
GeneID	1133.
KEGG	hsa:1133.
Organism-specific databases
H-InvDB	HIX0012084.
HGNC	HGNC:1954. CHRM5.
HPA	HPA013172.
MIM	118496. gene.
PharmGKB	PA26486.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOGENOM	P08912.
HOVERGEN	P08912.
Gene expression databases
ArrayExpress	P08912.
CleanEx	HS_CHRM5.
GermOnline	ENSG00000184984. Homo sapiens.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_supfam. IPR000502. Musac_M5_rcpt. IPR000995. Musac_rcpt. [Graphical view]
PANTHER	PTHR19266:SF36. Musac_M5_rcpt. 1 hit.
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR00243. MUSCARINICR. PR00542. MUSCRINICM5R.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProDom	P08912. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
DrugBank	DB00572. Atropine. DB00767. Benzquinamide. DB00785. Cryptenamine. DB00725. Homatropine Methylbromide. DB01403. Methotrimeprazine. DB00340. Metixene. DB00334. Olanzapine. DB00420. Promazine. DB01069. Promethazine. DB00777. Propiomazine. DB00372. Thiethylperazine.
SOURCE	Search...
ProtoNet	Search...

Entry information

Entry name

ACM5_HUMAN

Accession

Primary (citable) accession number: P08912
Secondary accession number(s): Q96RG7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	June 1, 1994
Last modified:	July 22, 2008
This is version 88 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)