Reviewed, UniProtKB/Swiss-Prot P09087 (ABDB_DROME)

Names and origin

Protein names

Recommended name:
    Homeobox protein abdominal-B
Alternative name(s):
    P3
    Infraabdominal 7
      Short name=IAB-7
    PH189

Gene names

Name:	Abd-B
ORF Names:	CG10291

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	493 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
Subcellular location	Nucleus.
Tissue specificity	Isoform M and isoform R are expressed in ectodermal and mesodermal tissues and central nervous system of fourth to ninth embryonic abdominal segments. Later in embryogenesis, expression is seen in visceral mesoderm surrounding hindgut and in two Malpighian tubules.
Developmental stage	Highly expressed during embryogenesis, lower expression in larvae, pupae and adults.
Sequence similarities	Belongs to the Abd-B homeobox family. Contains 1 homeobox DNA-binding domain.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Homeobox
Ligand	DNA-binding
Molecular function	Developmental protein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	determination of genital disc primordium Inferred from mutant phenotype. Source: FlyBase external genitalia morphogenesis Inferred from mutant phenotype. Source: FlyBase genital disc anterior/posterior pattern formation Inferred from expression pattern. Source: FlyBase genital disc sexually dimorphic development Inferred from mutant phenotype. Source: FlyBase gonadal mesoderm development Inferred from mutant phenotype. Source: FlyBase imaginal disc-derived female genitalia development Inferred from mutant phenotype. Source: FlyBase male genitalia development Inferred from mutant phenotype. Source: FlyBase male gonad development Inferred from mutant phenotype. Source: FlyBase midgut development Traceable author statement. Source: FlyBase negative regulation of cardioblast cell fate specification Inferred from mutant phenotype. Source: FlyBase negative regulation of salivary gland boundary specification Traceable author statement. Source: FlyBase negative regulation of striated muscle development Inferred from mutant phenotype. Source: FlyBase pole cell migration Traceable author statement. Source: FlyBase positive regulation of pigmentation during development Non-traceable author statement. Source: FlyBase sex-specific pigmentation Traceable author statement. Source: FlyBase specification of segmental identity, abdomen Inferred from mutant phenotype. Source: FlyBase spiracle morphogenesis, open tracheal system Inferred from mutant phenotype. Source: FlyBase
Molecular function	protein binding Inferred from physical interaction. Source: IntAct transcription factor activity Inferred from direct assay. Source: FlyBase
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	Q9VRG8	1	EBI-212395,EBI-127337

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform M (identifier: P09087-1)

Also known as: Morphogenetic; Abd-BI;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform R (identifier: P09087-2)

Also known as: Regulatory; Abd-BII;

The sequence of this isoform differs from the canonical sequence as follows:
     1-223: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 493	493	Homeobox protein abdominal-B
Regions
	DNA binding	387 – 446	60	Homeobox
	Compositional bias	1 – 192	192	Gln-rich
	Compositional bias	306 – 311	6	Poly-Ala
	Compositional bias	454 – 458	5	Poly-Asn
Natural variations
	Alternative sequence	1 – 223	223	Missing in isoform R.
Experimental info
	Sequence conflict	15	1	Q → H in CAB57859. Ref.4
	Sequence conflict	74	1	Missing in CAA34260. Ref.3
	Sequence conflict	177 – 180	4	ASGA → PVR in CAA34260. Ref.3
	Sequence conflict	317 – 318	2	ER → DG in CAA34260. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform M (Morphogenetic) (Abd-BI) [UniParc]. Last modified October 1, 1996. Version 3. Checksum: 55A11B5E97983A5F	FASTA	493	55,096
	10 20 30 40 50 60 MQQHHLQQQQ QQQQQQEQQH LQEQQQHLQQ LHHHAHHHLP QPLHTTSHHH SAHPHLQQQQ 70 80 90 100 110 120 QQQQHAVVAS SPSSVLQQQQ QQSTPTTHST PTHAVMYEDP PPVPLVAVQQ QHLPAPQQQQ 130 140 150 160 170 180 QLQQQQQQQQ QQLATTPVAG ALSPAQTPTG PSAQQQQHLT SPHHQQLPQQ QTPNSVASGA 190 200 210 220 230 240 SSNLQQQQQQ QNAAVAPGQT QIVAPTTASV SPSSVSSQKE DINMSIQLAP LHIPAIRAGP 250 260 270 280 290 300 GFETDTSAAV KRHTAHWAYN DEGFNQHYGS GYYDRKHMFA YPYPETQFPV GQYWGPNYRP 310 320 330 340 350 360 DQTTSAAAAA AYMNEAERHV SAAARQSVEG TSTSSYEPPT YSSPGGLRGY PSENYSSSGA 370 380 390 400 410 420 SGGLSVGAVG PCTPNPGLHE WTGQVSVRKK RKPYSKFQTL ELEKEFLFNA YVSKQKRWEL 430 440 450 460 470 480 ARNLQLTERQ VKIWFQNRRM KNKKNSQRQA NQQNNNNNSS SNHNHAQATQ QHHSGHHLNL 490 SLNMGHHAAK MHQ
	Isoform R (Regulatory) (Abd-BII) [UniParc]. Checksum: 3C951B3B406F1E49		270	30,344
	10 20 30 40 50 60 MSIQLAPLHI PAIRAGPGFE TDTSAAVKRH TAHWAYNDEG FNQHYGSGYY DRKHMFAYPY 70 80 90 100 110 120 PETQFPVGQY WGPNYRPDQT TSAAAAAAYM NEAERHVSAA ARQSVEGTST SSYEPPTYSS 130 140 150 160 170 180 PGGLRGYPSE NYSSSGASGG LSVGAVGPCT PNPGLHEWTG QVSVRKKRKP YSKFQTLELE 190 200 210 220 230 240 KEFLFNAYVS KQKRWELARN LQLTERQVKI WFQNRRMKNK KNSQRQANQQ NNNNNSSSNH 250 260 270 NHAQATQQHH SGHHLNLSLN MGHHAAKMHQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequence of the bithorax complex of Drosophila." Martin C.H., Mayeda C.A., Davis C.A., Ericsson C.L., Knafels J.D., Mathog D.R., Celniker S.E., Lewis E.B., Palazzolo M.J. Proc. Natl. Acad. Sci. U.S.A. 92:8398-8402(1995) [PubMed: 7667301] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS M AND R). Strain: Canton-S.
[2]	"Evidence that the Abdominal-B r element function is conferred by a trans-regulatory homeoprotein." Delorenzi M., Ali N., Saari G., Henry C., Wilcox M., Bienz M. EMBO J. 7:3223-3231(1988) [PubMed: 2903049] [Abstract] Cited for: NUCLEOTIDE SEQUENCE (ISOFORM R), CHARACTERIZATION. Tissue: Embryo.
[3]	"The molecular genetics of the bithorax complex of Drosophila: characterization of the products of the Abdominal-B domain." Celniker S.E., Keelan D.J., Lewis E.B. Genes Dev. 3:1424-1436(1989) [PubMed: 2575066] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM M), CHARACTERIZATION. Tissue: Embryo and Pupae.
[4]	"The morphogenetic and regulatory functions of the Drosophila Abdominal-B gene are encoded in overlapping RNAs transcribed from separate promoters." Zavortink M., Sakonju S. Genes Dev. 3:1969-1981(1989) [PubMed: 2482824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM M). Strain: Canton-S. Tissue: Embryo.
[5]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ISOFORMS M AND R). Strain: Berkeley.
[6]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[7]	"Homeo box genes of the Antennapedia and bithorax complexes of Drosophila." Regulski M., Harding K., Kostriken R., Karch F., Levine M., McGinnis W. Cell 43:71-80(1985) [PubMed: 2416463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 387-447.
[8]	"Different transcripts of the Drosophila Abd-B gene correlate with distinct genetic sub-functions." Kuziora M.A., McGinnis W. EMBO J. 7:3233-3244(1988) [PubMed: 2903050] [Abstract] Cited for: ALTERNATIVE SPLICING, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U31961 Genomic DNA. Translation: AAA84402.1. U31961 Genomic DNA. Translation: AAA84403.1. M21173 mRNA. Translation: AAA28400.1. X16134 mRNA. Translation: CAA34260.1. X51663 mRNA. Translation: CAB57859.1. AE014297 Genomic DNA. Translation: AAF55362.1. AE014297 Genomic DNA. Translation: AAF55363.1. X15080 mRNA. Translation: CAA33187.1.
PIR	A32584. A34220. B24780.
RefSeq	NP_524896.2. NP_650577.1.
UniGene	Dm.1763
3D structure databases
HSSP	HSSP built from PDB template 9ANT based on UniProtKB P02833.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:19566N.
IntAct	P09087.
Genome annotation databases
Ensembl	CG11648. Drosophila melanogaster. [Contig view]
GeneID	47763.
KEGG	dme:Dmel_CG11648.
NMPDR	fig|7227.3.peg.13214.
Organism-specific databases
FlyBase	FBgn0000015. Abd-B.
Phylogenomic databases
HOGENOM	P09087.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-011996-MON.
Gene expression databases
ArrayExpress	P09087.
GermOnline	CG11648. Drosophila melanogaster.
Family and domain databases
InterPro	IPR001356. Homeobox. IPR012287. Homeodomain-rel. IPR000047. HTH_lambrepressr. [Graphical view]
Gene3D	G3DSA:1.10.10.60. Homeodomain-rel. 1 hit.
Pfam	PF00046. Homeobox. 1 hit. [Graphical view]
PRINTS	PR00024. HOMEOBOX. PR00031. HTHREPRESSR.
ProDom	PD000010. Homeobox. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00389. HOX. 1 hit. [Graphical view]
PROSITE	PS00027. HOMEOBOX_1. 1 hit. PS50071. HOMEOBOX_2. 1 hit. [Graphical view]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

ABDB_DROME

Accession

Primary (citable) accession number: P09087
Secondary accession number(s): Q9VEQ8, Q9VEQ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	October 1, 1996
Last modified:	July 22, 2008
This is version 97 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project