Reviewed, UniProtKB/Swiss-Prot P09119 (CDC6_YEAST)

Names and origin

Protein names

Recommended name:
    Cell division control protein 6

Gene names

Name:	CDC6
Ordered Locus Names:	YJL194W
ORF Names:	J0347

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	513 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Appears to have an important and perhaps unique dual role in S phase, it is first required for the initiation of DNA replication and then actively participates in the suppression of nuclear division. Interacts with the origin recognition complex (ORC). Targeted for degradation by the E3 ubiquitin ligase complex SCF(Cdc4).
Subunit structure	Interacts with CDC4.
Subcellular location	Cell membrane.
Sequence similarities	Belongs to the CDC6/cdc18 family.

Ontologies

Keywords
Biological process	Cell cycle Cell division DNA replication
Cellular component	Cell membrane Membrane
Ligand	ATP-binding Nucleotide-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	G1/S transition of mitotic cell cycle Inferred from mutant phenotype. Source: SGD cell division Inferred from electronic annotation. Source: UniProtKB-KW pre-replicative complex assembly Inferred from direct assay. Source: SGD regulation of DNA replication initiation Inferred from mutant phenotype. Source: SGD regulation of chromatin silencing at telomere Inferred from mutant phenotype. Source: SGD
Cellular component	DNA replication preinitiation complex Inferred from direct assay. Source: SGD plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW pre-replicative complex Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATPase activity Inferred from direct assay. Source: SGD DNA replication origin binding Inferred from direct assay. Source: SGD GTP binding Inferred from direct assay. Source: SGD GTPase activity Inferred from direct assay. Source: SGD chromatin binding Inferred from direct assay. Source: SGD protein binding Ref.8 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
LSB3	P43603	1	EBI-4447,EBI-22980

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 513	513	Cell division control protein 6		PRO_0000150977
Regions
	Nucleotide binding	108 – 115	8	ATP Potential
	Region	1 – 47	47	Interaction with CDC4; required for degradation
Experimental info
	Sequence conflict	3	1	A → G in AAA34484. Ref.1
	Sequence conflict	272	1	L → P in CAA31510. Ref.7
	Sequence conflict	395 – 396	2	SK → LQN in CAA31510. Ref.7
	Sequence conflict	402	1	S → L in CAA31510. Ref.7
	Sequence conflict	410	1	L → S in CAA31510. Ref.7
	Sequence conflict	436	1	S → L in CAA31510. Ref.7

Sequences

	Sequence		Length	Mass (Da)
	P09119-1 [UniParc]. Last modified February 1, 1995. Version 3. Checksum: 13645F65BEAB5EE8	FASTA	513	58,037
	10 20 30 40 50 60 MSAIPITPTK RIRRNLFDDA PATPPRPLKR KKLQFTDVTP ESSPEKLQFG SQSIFLRTKA 70 80 90 100 110 120 LLQKSSELVN LNSSDGALPA RTAEYEQVMN FLAKAISEHR SDSLYITGPP GTGKTAQLDM 130 140 150 160 170 180 IIRQKFQSLP LSLSTPRSKD VLRHTNPNLQ NLSWFELPDG RLESVAVTSI NCISLGEPSS 190 200 210 220 230 240 IFQKIFDSFQ DLNGPTLQIK NMQHLQKFLE PYHKKTTFVV VLDEMDRLLH ANTSETQSVR 250 260 270 280 290 300 TILELFLLAK LPTVSFVLIG MANSLDMKDR FLSRLNLDRG LLPQTIVFQP YTAEQMYEIV 310 320 330 340 350 360 IQKMSSLPTI IFQPMAIKFA AKKCAGNTGD LRKLFDVLRG SIEIYELEKR FLLSPTRGSL 370 380 390 400 410 420 NSAQVPLTPT TSPVKKSYPE PQGKIGLNYI AKVFSKFVNN NSTRTRIAKL NIQQKLILCT 430 440 450 460 470 480 IIQSLKLNSD ATIDESFDHY IKAITKTDTL APLQRNEFLE ICTILETCGL VSIKKTKCKG 490 500 510 KTKRFVDKID VDLDMREFYD EMTKISILKP FLH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of Saccharomyces cerevisiae CDC6 gene. Isolation, identification, and sequence analysis." Zhou C., Huang S.H., Jong A.Y. J. Biol. Chem. 264:9022-9029(1989) [PubMed: 2656692] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Molecular cloning and characterization of the cell division cycle gene CDC6 from Saccharomyces cerevisiae." Colasanti J.J., Comer A.R., Bruschi C.V. Submitted (APR-1990) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Dual functions of CDC6: a yeast protein required for DNA replication also inhibits nuclear division." Bueno A., Russell P. EMBO J. 11:2167-2176(1992) [PubMed: 1600944] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[4]	"The sequence of a 36 kb segment on the left arm of yeast chromosome X identifies 24 open reading frames including NUC1, PRP21 (SPP91), CDC6, CRY2, the gene for S24, a homologue to the aconitase gene ACO1 and two homologues to chromosome III genes." Purnelle B., Coster F., Goffeau A. Yeast 10:1235-1249(1994) [PubMed: 7754713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[6]	"CDC6 mRNA fluctuates periodically in the yeast cell cycle." Zhou C., Jong A.Y. J. Biol. Chem. 265:19904-19909(1990) [PubMed: 2246267] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-48.
[7]	"Cloning and characterization of the Saccharomyces cerevisiae CDC6 gene." Lisziewicz J., Godany A., Agoston D.V., Kuentzel H. Nucleic Acids Res. 16:11507-11520(1988) [PubMed: 3062576] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 89-513.
[8]	"The Cdc4/34/53 pathway targets Cdc6p for proteolysis in budding yeast." Drury L.S., Perkins G., Diffley J.F. EMBO J. 16:5966-5976(1997) [PubMed: 9312054] [Abstract] Cited for: FUNCTION, INTERACTION WITH CDC4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	J04734 Genomic DNA. Translation: AAA34484.1. Sequence problems. M22858 Genomic DNA. Translation: AAA34483.1. X65299 Genomic DNA. Translation: CAA46392.1. X77688 Genomic DNA. Translation: CAA54766.1. Z49470 Genomic DNA. Translation: CAA89490.1. M61183 Genomic DNA. Translation: AAA34488.1. X13118 Genomic DNA. Translation: CAA31510.1.
PIR	RGBYC6. S46640.
RefSeq	NP_012341.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:2267N.
IntAct	P09119.
Genome annotation databases
Ensembl	YJL194W. Saccharomyces cerevisiae. [Contig view]
GeneID	853244.
GenomeReviews	Gene locus YJL194W in contig Y13136_GR.
KEGG	sce:YJL194W.
NMPDR	fig|4932.3.peg.3305.
Organism-specific databases
CYGD	YJL194w.
SGD	S000003730. CDC6.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P09119.
Gene expression databases
ArrayExpress	P09119.
GermOnline	YJL194W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR003593. AAA+_ATPase_core. IPR016314. Cdc6. [Graphical view]
PIRSF	PIRSF001767. Cdc6. 1 hit.
SMART	SM00382. AAA. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
LinkHub	P09119.
NextBio	973478.

Entry information

Entry name

CDC6_YEAST

Accession

Primary (citable) accession number: P09119

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	February 1, 1995
Last modified:	November 25, 2008
This is version 77 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)