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P09372

- GRPE_ECOLI

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Protein
Protein GrpE
Gene
grpE, b2614, JW2594

Gene:

grpE, b2614, JW2594
Organism
Escherichia coli (strain K12)
Status
Reviewed - - Experimental evidence at protein leveli

UniProt

P09372 - GRPE_ECOLI

Protein:

Protein GrpE
Protein Existence: Experimental evidence at protein level

Functioni

Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with DnaK and GrpE. It is the nucleotide exchange factor for DnaK and may function as a thermosensor. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding.4 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier
Sitei1831Interaction with DnaK

GO - Molecular functioni

  1. adenyl-nucleotide exchange factor activityInferred from electronic annotationi Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. adenyl-nucleotide exchange factor activityInferred from electronic annotationi Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Stress response

Enzyme and pathway databases

BioCyciEcoCyc:EG10416-MONOMER.
ECOL316407:JW2594-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein GrpE
Alternative name(s):
HSP-70 cofactor
HSP24
Heat shock protein B25.3
Gene namesi
Name:grpE
Ordered Locus Names:b2614, JW2594
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10416. grpE.

Subcellular locationi

Cytoplasm Inferred UniRule annotation

GO - Cellular componenti

  1. cytoplasmInferred from direct assayi PubMed 16858726 Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier
Mutagenesisi731R → A: Great decrease in ability to interact with DnaK. 1 Publication
Mutagenesisi741R → A: Great decrease in ability to interact with DnaK. 1 Publication
Mutagenesisi821K → A: Great decrease in ability to interact with DnaK. 1 Publication
Mutagenesisi861F → A: No effect in ability to interact with DnaK. 1 Publication
Mutagenesisi1041R → A: No effect in ability to interact with DnaK. 1 Publication
Mutagenesisi1071E → A: No effect in ability to interact with DnaK. 1 Publication
Mutagenesisi1081V → A: No effect in ability to interact with DnaK. 1 Publication
Mutagenesisi1221G → D: Temperature-sensitive phenotype. 1 Publication
Mutagenesisi1491L → A: No effect in ability to interact with DnaK. 1 Publication
Mutagenesisi1511P → A: No effect in ability to interact with DnaK. 1 Publication
Mutagenesisi1551Q → A: No effect in ability to interact with DnaK. 1 Publication
Mutagenesisi1571I → A: No effect in ability to interact with DnaK. 1 Publication
Mutagenesisi1591M → A: No effect in ability to interact with DnaK. 1 Publication
Mutagenesisi1741M → A: No effect in ability to interact with DnaK. 1 Publication
Mutagenesisi1831R → A: Loss of ability to interact with DnaK. 2 Publications
Mutagenesisi1861R → A: No effect in ability to interact with DnaK.
Mutagenesisi1891M → A: No effect in ability to interact with DnaK.
Mutagenesisi1921V → A: Loss of ability to interact with DnaK. 2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier
Chaini1 – 197197Protein GrpEUniRule annotation
PRO_0000113782

Proteomic databases

PaxDbiP09372.
PRIDEiP09372.

2D gel databases

SWISS-2DPAGEiP09372.

Expressioni

Inductioni

By the sigma(32) subunit of RNA polymerase.UniRule annotation

Gene expression databases

GenevestigatoriP09372.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi851433. 1 interaction.
DIPiDIP-6141N.
IntActiP09372. 49 interactions.
MINTiMINT-1225400.
STRINGi511145.b2614.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier
Helixi39 – 8547
Helixi88 – 903
Helixi91 – 944
Helixi96 – 10712
Helixi117 – 13418
Turni135 – 1384
Beta strandi139 – 1424
Beta strandi146 – 1483
Beta strandi153 – 1619
Beta strandi163 – 1653
Beta strandi169 – 1757
Beta strandi177 – 1804
Beta strandi183 – 1864
Beta strandi188 – 1947

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DKGX-ray2.80A/B1-197[»]
DisProtiDP00103.
ProteinModelPortaliP09372.
SMRiP09372. Positions 34-197.

Miscellaneous databases

EvolutionaryTraceiP09372.

Family & Domainsi

Sequence similaritiesi

Belongs to the GrpE family.

Phylogenomic databases

eggNOGiCOG0576.
HOGENOMiHOG000252084.
KOiK03687.
OMAiMSMMESE.
OrthoDBiEOG6FJNJ9.
PhylomeDBiP09372.
ProtClustDBiPRK10325.

Family and domain databases

Gene3Di2.30.22.10. 1 hit.
3.90.20.20. 1 hit.
HAMAPiMF_01151. GrpE.
InterProiIPR000740. GrpE.
IPR013805. GrpE_coiled_coil.
IPR009012. GrpE_head.
[Graphical view]
PANTHERiPTHR21237. PTHR21237. 1 hit.
PfamiPF01025. GrpE. 1 hit.
[Graphical view]
PRINTSiPR00773. GRPEPROTEIN.
SUPFAMiSSF51064. SSF51064. 1 hit.
PROSITEiPS01071. GRPE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P09372-1 [UniParc]FASTA

« Hide

MSSKEQKTPE GQAPEEIIMD QHEEIEAVEP EASAEQVDPR DEKVANLEAQ    50
LAEAQTRERD GILRVKAEME NLRRRTELDI EKAHKFALEK FINELLPVID 100
SLDRALEVAD KANPDMSAMV EGIELTLKSM LDVVRKFGVE VIAETNVPLD 150
PNVHQAIAMV ESDDVAPGNV LGIMQKGYTL NGRTIRAAMV TVAKAKA 197
Length:197
Mass (Da):21,798
Last modified:July 1, 1989 - v1
Checksum:iCDC4CD9D08AD4BEF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X07863 Genomic DNA. Translation: CAA30711.1.
U00096 Genomic DNA. Translation: AAC75663.1.
AP009048 Genomic DNA. Translation: BAA16498.1.
PIRiS01240.
RefSeqiNP_417104.1. NC_000913.3.
YP_490836.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC75663; AAC75663; b2614.
BAA16498; BAA16498; BAA16498.
GeneIDi12931622.
947097.
KEGGiecj:Y75_p2561.
eco:b2614.
PATRICi32120625. VBIEscCol129921_2711.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X07863 Genomic DNA. Translation: CAA30711.1 .
U00096 Genomic DNA. Translation: AAC75663.1 .
AP009048 Genomic DNA. Translation: BAA16498.1 .
PIRi S01240.
RefSeqi NP_417104.1. NC_000913.3.
YP_490836.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1DKG X-ray 2.80 A/B 1-197 [» ]
DisProti DP00103.
ProteinModelPortali P09372.
SMRi P09372. Positions 34-197.
ModBasei Search...

Protein-protein interaction databases

BioGridi 851433. 1 interaction.
DIPi DIP-6141N.
IntActi P09372. 49 interactions.
MINTi MINT-1225400.
STRINGi 511145.b2614.

Chemistry

ChEMBLi CHEMBL1293284.

2D gel databases

SWISS-2DPAGEi P09372.

Proteomic databases

PaxDbi P09372.
PRIDEi P09372.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC75663 ; AAC75663 ; b2614 .
BAA16498 ; BAA16498 ; BAA16498 .
GeneIDi 12931622.
947097.
KEGGi ecj:Y75_p2561.
eco:b2614.
PATRICi 32120625. VBIEscCol129921_2711.

Organism-specific databases

EchoBASEi EB0411.
EcoGenei EG10416. grpE.

Phylogenomic databases

eggNOGi COG0576.
HOGENOMi HOG000252084.
KOi K03687.
OMAi MSMMESE.
OrthoDBi EOG6FJNJ9.
PhylomeDBi P09372.
ProtClustDBi PRK10325.

Enzyme and pathway databases

BioCyci EcoCyc:EG10416-MONOMER.
ECOL316407:JW2594-MONOMER.

Miscellaneous databases

EvolutionaryTracei P09372.
PROi P09372.

Gene expression databases

Genevestigatori P09372.

Family and domain databases

Gene3Di 2.30.22.10. 1 hit.
3.90.20.20. 1 hit.
HAMAPi MF_01151. GrpE.
InterProi IPR000740. GrpE.
IPR013805. GrpE_coiled_coil.
IPR009012. GrpE_head.
[Graphical view ]
PANTHERi PTHR21237. PTHR21237. 1 hit.
Pfami PF01025. GrpE. 1 hit.
[Graphical view ]
PRINTSi PR00773. GRPEPROTEIN.
SUPFAMi SSF51064. SSF51064. 1 hit.
PROSITEi PS01071. GRPE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publicationsDownload
  1. "Sequence analysis and transcriptional regulation of the Escherichia coli grpE gene, encoding a heat shock protein."
    Lipinska B., King J., Ang D., Georgopoulos C.
    Nucleic Acids Res. 16:7545-7562(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: B178.
  2. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
    Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
    , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
    DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "The complete genome sequence of Escherichia coli K-12."
    Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
    Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Escherichia coli DnaJ and GrpE heat shock proteins jointly stimulate ATPase activity of DnaK."
    Liberek K., Marszalek J., Ang D., Georgopoulos C., Zylicz M.
    Proc. Natl. Acad. Sci. U.S.A. 88:2874-2878(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: POSSIBLE FUNCTION.
  6. "Structure-function analysis of the Escherichia coli GrpE heat shock protein."
    Wu B., Wawrzynow A., Zylicz M., Georgopoulos C.
    EMBO J. 15:4806-4816(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS.
  7. "Escherichia coli proteome analysis using the gene-protein database."
    VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
    Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY 2D-GEL.
  8. "A GrpE mutant containing the NH(2)-terminal 'tail' region is able to displace bound polypeptide substrate from DnaK."
    Mehl A.F., Heskett L.D., Neal K.M.
    Biochem. Biophys. Res. Commun. 282:562-569(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: POSSIBLE ROLE IN PEPTIDE RELEASE FROM DNAK.
  9. "Thermosensor action of GrpE. The DnaK chaperone system at heat shock temperatures."
    Grimshaw J.P., Jelesarov I., Siegenthaler R.K., Christen P.
    J. Biol. Chem. 278:19048-19053(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: POSSIBLE ROLE AS A THERMOSENSOR.
  10. "Influence of GrpE on DnaK-substrate interactions."
    Brehmer D., Gaessler C., Rist W., Mayer M.P., Bukau B.
    J. Biol. Chem. 279:27957-27964(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: ROLE IN DNAK INTERACTION WITH SUBSTRATE.
  11. "Thermodynamic linkage in the GrpE nucleotide exchange factor, a molecular thermosensor."
    Gelinas A.D., Toth J., Bethoney K.A., Langsetmo K., Stafford W.F., Harrison C.J.
    Biochemistry 42:9050-9059(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF PHE-86; ARG-183 AND VAL-192.
  12. "Mutational analysis of the energetics of the GrpE.DnaK binding interface: equilibrium association constants by sedimentation velocity analytical ultracentrifugation."
    Gelinas A.D., Toth J., Bethoney K.A., Stafford W.F., Harrison C.J.
    J. Mol. Biol. 339:447-458(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF ARG-73; ARG-74; LYS-82; ARG-104; GLU-107; VAL-108; LEU-149; PRO-151; GLN-155; ILE-157; MET-159; MET-174; ARG-183 AND VAL-192.
  13. "Crystal structure of the nucleotide exchange factor GrpE bound to the ATPase domain of the molecular chaperone DnaK."
    Harrison C.J., Hayer-Hartl M., di Liberto M., Hartl F.-U., Kuriyan J.
    Science 276:431-435(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) IN COMPLEX WITH ATPASE DOMAIN OF DNAK, MUTAGENESIS OF GLY-122.

Entry nameiGRPE_ECOLI
AccessioniPrimary (citable) accession number: P09372
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: April 16, 2014
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene

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