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P09570

- NIFA_AZOVI

UniProt

P09570 - NIFA_AZOVI

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Protein
Nif-specific regulatory protein
Gene
nifA
Organism
Azotobacter vinelandii
Status
Reviewed - Annotation score: 3 out of 5 - Protein predictedi

Functioni

Required for activation of most nif operons, which are directly involved in nitrogen fixation.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi239 – 2468ATP Reviewed prediction
Nucleotide bindingi302 – 31110ATP Reviewed prediction
DNA bindingi494 – 51320H-T-H motif By similarity
Add
BLAST

GO - Molecular functioni

  1. ATP bindingInferred from electronic annotationi Source: UniProtKB-KW
  2. nucleoside-triphosphatase activityInferred from electronic annotationi Source: InterPro
  3. sequence-specific DNA bindingInferred from electronic annotationi Source: InterPro
  4. sequence-specific DNA binding transcription factor activityInferred from electronic annotationi Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. nitrogen fixationInferred from electronic annotationi Source: UniProtKB-KW
  2. phosphorelay signal transduction systemInferred from electronic annotationi Source: UniProtKB-KW
  3. transcription, DNA-templatedInferred from electronic annotationi Source: UniProtKB-KW
  1. nucleoside-triphosphatase activityInferred from electronic annotationi Source: InterPro
  2. sequence-specific DNA binding transcription factor activityInferred from electronic annotationi Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Nitrogen fixation, Transcription, Transcription regulation, Two-component regulatory system

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Nif-specific regulatory protein
Gene namesi
Name:nifA
OrganismiAzotobacter vinelandii
Taxonomic identifieri354 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaeAzotobacter

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 522522Nif-specific regulatory protein
PRO_0000081304Add
BLAST

Interactioni

Subunit structurei

Interacts with sigma-54.

Protein-protein interaction databases

IntActiP09570. 1 interaction.
MINTiMINT-107513.

Structurei

3D structure databases

ProteinModelPortaliP09570.
SMRiP09570. Positions 210-452.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini37 – 178142GAF
Add
BLAST
Domaini211 – 439229Sigma-54 factor interaction
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 184184A domain
Add
BLAST
Regioni440 – 47940Inter-domain linker
Add
BLAST
Regioni480 – 52243C-terminal DNA-binding domain
Add
BLAST

Sequence similaritiesi

Contains 1 GAF domain.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
3.30.450.40. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003018. GAF.
IPR029016. GAF_dom_like.
IPR009057. Homeodomain-like.
IPR002197. HTH_Fis.
IPR010113. Nif-specific_regulatory_prot.
IPR027417. P-loop_NTPase.
IPR002078. Sigma_54_int.
IPR025662. Sigma_54_int_dom_ATP-bd_1.
IPR025943. Sigma_54_int_dom_ATP-bd_2.
IPR025944. Sigma_54_int_dom_CS.
[Graphical view]
PfamiPF01590. GAF. 1 hit.
PF02954. HTH_8. 1 hit.
PF00158. Sigma54_activat. 1 hit.
[Graphical view]
PRINTSiPR01590. HTHFIS.
SMARTiSM00382. AAA. 1 hit.
SM00065. GAF. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF55781. SSF55781. 1 hit.
TIGRFAMsiTIGR01817. nifA. 1 hit.
PROSITEiPS00675. SIGMA54_INTERACT_1. 1 hit.
PS00676. SIGMA54_INTERACT_2. 1 hit.
PS00688. SIGMA54_INTERACT_3. 1 hit.
PS50045. SIGMA54_INTERACT_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P09570-1 [UniParc]FASTA

« Hide

MNATIPQRSA KQNPVELYDL QLQALASIAR TLSREQQIDE LLEQVLAVLH    50
NDLGLLHGLV TISDPEHGAL QIGAIHTDSE AVAQACEGVR YRSGEGVIGN 100
VLKHGNSVVL GRISADPRFL DRLALYDLEM PFIAVPIKNP EGNTIGVLAA 150
QPDCRADEHM PARTRFLEIV ANLLAQTVRL VVNIEDGREA ADERDELRRE 200
VRGKYGFENM VVGHTPTMRR VFDQIRRVAK WNSTVLVLGE SGTGKELIAS 250
AIHYKSPRAH RPFVRLNCAA LPETLLESEL FGHEKGAFTG AVKQRKGRFE 300
QADGGTLFLD EIGEISPMFQ AKLLRVLQEG EFERVGGNQT VRVNVRIVAA 350
TNRDLESEVE KGKFREDLYY RLNVMAIRIP PLRERTADIP ELAEFLLGKI 400
GRQQGRPLTV TDSAIRLLMS HRWPGNVREL ENCLERSAIM SEDGTITRDV 450
VSLTGVDNES PPLAAPLPEV NLADETLDDR ERVIAALEQA GWVQAKAARL 500
LGMTPRQIAY RIQTLNIHMR KI 522
Length:522
Mass (Da):58,123
Last modified:July 1, 1989 - v1
Checksum:i9556A07EE8A7B209
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y00554 Genomic DNA. Translation: CAA68633.1.
J03411 Genomic DNA. Translation: AAA22147.1.
PIRiS01927.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y00554 Genomic DNA. Translation: CAA68633.1 .
J03411 Genomic DNA. Translation: AAA22147.1 .
PIRi S01927.

3D structure databases

ProteinModelPortali P09570.
SMRi P09570. Positions 210-452.
ModBasei Search...

Protein-protein interaction databases

IntActi P09570. 1 interaction.
MINTi MINT-107513.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.10.10.60. 1 hit.
3.30.450.40. 1 hit.
3.40.50.300. 1 hit.
InterProi IPR003593. AAA+_ATPase.
IPR003018. GAF.
IPR029016. GAF_dom_like.
IPR009057. Homeodomain-like.
IPR002197. HTH_Fis.
IPR010113. Nif-specific_regulatory_prot.
IPR027417. P-loop_NTPase.
IPR002078. Sigma_54_int.
IPR025662. Sigma_54_int_dom_ATP-bd_1.
IPR025943. Sigma_54_int_dom_ATP-bd_2.
IPR025944. Sigma_54_int_dom_CS.
[Graphical view ]
Pfami PF01590. GAF. 1 hit.
PF02954. HTH_8. 1 hit.
PF00158. Sigma54_activat. 1 hit.
[Graphical view ]
PRINTSi PR01590. HTHFIS.
SMARTi SM00382. AAA. 1 hit.
SM00065. GAF. 1 hit.
[Graphical view ]
SUPFAMi SSF46689. SSF46689. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF55781. SSF55781. 1 hit.
TIGRFAMsi TIGR01817. nifA. 1 hit.
PROSITEi PS00675. SIGMA54_INTERACT_1. 1 hit.
PS00676. SIGMA54_INTERACT_2. 1 hit.
PS00688. SIGMA54_INTERACT_3. 1 hit.
PS50045. SIGMA54_INTERACT_4. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

Download
  1. "Nucleotide sequence and mutagenesis of the nifA gene from Azotobacter vinelandii."
    Bennet L.T., Cannon F., Dean D.R.
    Mol. Microbiol. 2:315-321(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 13705 / OP1 / DSM 366 / NCIB 11614 / LMG 3878 / UW.
  2. "Nucleotide sequence and genetic analysis of the nifB-nifQ region from Azotobacter vinelandii."
    Joerger R.D., Bishop P.E.
    J. Bacteriol. 170:1475-1487(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 394-522.

Entry nameiNIFA_AZOVI
AccessioniPrimary (citable) accession number: P09570
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: June 11, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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