T-cell surface glycoprotein CD3 gamma chain precursor

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Reviewed, UniProtKB/Swiss-Prot P09693 (CD3G_HUMAN)

Last modified July 22, 2008. Version 83. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    T-cell surface glycoprotein CD3 gamma chain
Alternative name(s):
    T-cell receptor T3 gamma chain
    CD_antigen=CD3g

Gene names

Name:	CD3G
Synonyms:	T3G

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	182 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	The CD3 complex mediates signal transduction.
Subunit structure	The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta.
Subcellular location	Membrane; Single-pass type I membrane protein.
Domain	A di-leucine motif and a tyrosine-based motif are individually sufficient to induce both endocytosis and delivery to lysosomes.
Sequence similarities	Contains 1 Ig-like (immunoglobulin-like) domain. Contains 1 ITAM domain.

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Ontologies

Keywords
Cellular component	Membrane
Domain	Immunoglobulin domain Signal Transmembrane
Molecular function	Receptor
PTM	Glycoprotein Phosphoprotein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	T cell activation Non-traceable author statement. Source: UniProtKB cell surface receptor linked signal transduction Inferred from mutant phenotype. Source: UniProtKB establishment or maintenance of cell polarity Inferred from mutant phenotype. Source: UniProtKB protein complex assembly Non-traceable author statement. Source: UniProtKB protein transport Inferred from mutant phenotype. Source: UniProtKB regulation of apoptosis Inferred from mutant phenotype. Source: UniProtKB
Cellular component	T cell receptor complex Non-traceable author statement. Source: UniProtKB integral to plasma membrane Non-traceable author statement. Source: UniProtKB
Molecular function	T cell receptor binding Non-traceable author statement. Source: UniProtKB protein heterodimerization activity Inferred from physical interaction. Source: UniProtKB receptor signaling complex scaffold activity Non-traceable author statement. Source: UniProtKB transmembrane receptor activity Inferred from mutant phenotype. Source: UniProtKB
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Molecule processing

Signal peptide

1 – 22

Chain

23 – 182

160

T-cell surface glycoprotein CD3 gamma chain

Regions

Topological domain

23 – 116

Extracellular Potential

Transmembrane

117 – 137

Potential

Topological domain

138 – 182

Cytoplasmic Potential

Domain

37 – 94

Ig-like

Domain

149 – 177

ITAM

Motif

153 – 154

Di-leucine motif

Amino acid modifications

Modified residue

145

Phosphoserine

Modified residue

148

Phosphoserine

Modified residue

160

Phosphotyrosine

Modified residue

171

Phosphotyrosine

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

N-linked (GlcNAc...) Potential

Disulfide bond

46 ↔ 87

By similarity

Experimental info

Mutagenesis

153

L → A: Abolishes lysosomal targeting

Mutagenesis

153

L → I: Diminished but persistent lysosomal targeting

Mutagenesis

154

L → A: Diminished but persistent lysosomal targeting

Mutagenesis

154

L → I: No effect

Mutagenesis

160

Y → A: Abolishes lysosomal targeting

Mutagenesis

163

L → A: Abolishes lysosomal targeting

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Sequences

Sequence

Length

Mass (Da)

Tools

P09693-1 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: EE65C0186FB9872B
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FASTA

182

20,469

        10         20         30         40         50         60 
MEQGKGLAVL ILAIILLQGT LAQSIKGNHL VKVYDYQEDG SVLLTCDAEA KNITWFKDGK 

        70         80         90        100        110        120 
MIGFLTEDKK KWNLGSNAKD PRGMYQCKGS QNKSKPLQVY YRMCQNCIEL NAATISGFLF 

       130        140        150        160        170        180 
AEIVSIFVLA VGVYFIAGQD GVRQSRASDK QTLLPNDQLY QPLKDREDDQ YSHLQGNQLR 


RN

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Primary structure of the T3 gamma subunit of the T3/T cell antigen receptor complex deduced from cDNA sequences: evolution of the T3 gamma and delta subunits." Krissansen G.W., Owen M.J., Verbi W., Crumpton M.J. EMBO J. 5:1799-1808(1986) [PubMed: 2944745] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Physical linkage of three CD3 genes on human chromosome 11." Tunnacliffe A., Buluwela L., Rabbitts T.H. EMBO J. 6:2953-2957(1987) [PubMed: 2826124] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Dephosphorylation of the human T lymphocyte CD3 antigen." Alexander D., Goris J., Marais R., Rothbard J., Merlevede W., Crumpton M.J. Eur. J. Biochem. 181:55-65(1989) [PubMed: 2540970] [Abstract] Cited for: PROTEIN SEQUENCE OF 139-182, PHOSPHORYLATION AT SER-145 AND SER-148.
[5]	"The human T3 gamma chain is phosphorylated at serine 126 in response to T lymphocyte activation." Davies A.A., Cantrell D.A., Hexham J.M., Parker P.J., Rothbard J., Crumpton M.J. J. Biol. Chem. 262:10918-10921(1987) [PubMed: 3112151] [Abstract] Cited for: PHOSPHORYLATION AT SER-145 AND SER-148.
[6]	"A novel di-leucine motif and a tyrosine-based motif independently mediate lysosomal targeting and endocytosis of CD3 chains." Letourneur F., Klausner R.D. Cell 69:1143-1157(1992) [PubMed: 1535555] [Abstract] Cited for: MUTAGENESIS OF LEU-153; LEU-154; TYR-160 AND LEU-163.
[7]	"Quantitative phosphoproteome analysis using a dendrimer conjugation chemistry and tandem mass spectrometry." Tao W.A., Wollscheid B., O'Brien R., Eng J.K., Li X.-J., Bodenmiller B., Watts J.D., Hood L., Aebersold R. Nat. Methods 2:591-598(2005) [PubMed: 16094384] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-160 AND TYR-171, MASS SPECTROMETRY. Tissue: T-cell.
+	Additional computationally mapped references.

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Web resources

CD3Gbase

CD3G mutation db

Wikipedia

CD3 receptor entry

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Cross-references

Sequence databases

X04145 mRNA. Translation: CAA27764.1.
X06026

X06031 Genomic DNA. Translation: CAA29428.1.
BC113830 mRNA. Translation: AAI13831.1.

PIR

A25468.

RefSeq

NP_000064.1.

UniGene

Hs.2259

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1SY6	X-ray	2.10	A	23-103	[»]

SMR

P09693. Positions 23-103.

DisProt

DP00508.

ModBase

Search...

PTM databases

PhosphoSite

P09693.

Proteomic databases

PeptideAtlas

P09693.

Genome annotation databases

Ensembl

ENSG00000160654. Homo sapiens. [Contig view]

GeneID

917.

KEGG

hsa:917.

Organism-specific databases

H-InvDB

HIX0036000.

HGNC

HGNC:1675. CD3G.

HPA

CAB017520.

MIM

186740. gene+phenotype.

PharmGKB

PA26217.

GenAtlas

Search...

GeneCards

Search...

Phylogenomic databases

HOGENOM

P09693.

HOVERGEN

P09693.

Enzyme and pathway databases

Reactome

REACT_6900. Signaling in Immune System.

Gene expression databases

ArrayExpress

P09693.

CleanEx

HS_CD3G.

GermOnline

ENSG00000160654. Homo sapiens.

Family and domain databases

InterPro

IPR015484. CD3_gamma_delta.
IPR007110. Ig-like.
IPR013783. Ig-like_fold.
IPR003598. Ig_sub2.
IPR003110. Phos_rcpt_ITAM.
[Graphical view]

Gene3D

G3DSA:2.60.40.10. Ig-like_fold. 1 hit.

PANTHER

PTHR10570. CD3_gamma_delta. 1 hit.

Pfam

PF02189. ITAM. 1 hit.
[Graphical view]

SMART

SM00408. IGc2. 1 hit.
SM00077. ITAM. 1 hit.
[Graphical view]

PROSITE

PS50835. IG_LIKE. False negative.
PS51055. ITAM_1. 1 hit.
[Graphical view]

ProDom

P09693.
[Graphical view] [Entries sharing at least one domain]

BLOCKS

Search...

Other Resources

SOURCE

Search...

ProtoNet

Search...

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Entry information

Entry name

CD3G_HUMAN

Accession

Primary (citable) accession number: P09693
Secondary accession number(s): Q2HIZ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	July 22, 2008
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

Human cell differentiation molecules

CD nomenclature of surface proteins of human leucocytes and list of entries

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

UniProtKB secondary accession numbers

Index of UniProtKB secondary accession numbers

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Keywords

Gene Ontology (GO)

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Web resources

Cross-references

Sequence databases

3D structure databases

PTM databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents