Reviewed, UniProtKB/Swiss-Prot P09850 (XYNA_BACCI)

Names and origin

Protein names

Recommended name:
    Endo-1,4-beta-xylanase
      Short name=Xylanase
    EC=3.2.1.8
Alternative name(s):
    1,4-beta-D-xylan xylanohydrolase

Gene names

Name:

xlnA

Organism

Bacillus circulans

Taxonomic identifier

1397 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	213 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.
Pathway	Glycan degradation; xylan degradation.
Sequence similarities	Belongs to the glycosyl hydrolase 11 (cellulase G) family.

Ontologies

Keywords
Biological process	Xylan degradation
Domain	Signal
Molecular function	Glycosidase Hydrolase
Technical term	3D-structure
Gene Ontology (GO)
Biological process	xylan catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	endo-1,4-beta-xylanase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 28

28

Chain

29 – 213

185

Endo-1,4-beta-xylanase

PRO_0000007996

Sites

Active site

106

1

Nucleophile

Active site

200

1

Proton donor

Secondary structure

1

.

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.

.

.

.

.

.

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.

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.

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213

Helix Strand Turn

Details...

Beta strand

33 – 38

6

Beta strand

42 – 48

7

Beta strand

53 – 60

8

Beta strand

62 – 72

11

Beta strand

78 – 101

24

Turn

102 – 104

3

Beta strand

105 – 115

11

Beta strand

121 – 128

8

Beta strand

131 – 144

14

Beta strand

148 – 162

15

Beta strand

166 – 168

3

Beta strand

170 – 173

4

Helix

174 – 183

10

Beta strand

190 – 203

14

Sequences

	Sequence		Length	Mass (Da)
	P09850-1 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 4BA0A35238CC0135	FASTA	213	23,359
	10 20 30 40 50 60 MFKFKKNFLV GLSAALMSIS LFSATASAAS TDYWQNWTDG GGIVNAVNGS GGNYSVNWSN 70 80 90 100 110 120 TGNFVVGKGW TTGSPFRTIN YNAGVWAPNG NGYLTLYGWT RSPLIEYYVV DSWGTYRPTG 130 140 150 160 170 180 TYKGTVKSDG GTYDIYTTTR YNAPSIDGDR TTFTQYWSVR QSKRPTGSNA TITFTNHVNA 190 200 210 WKSHGMNLGS NWAYQVMATE GYQSSGSSNV TVW

References

[1]	"Nucleotide sequence of a Bacillus circulans xylanase gene." Yang R.C.A., MacKenzie C.R., Narang S.A. Nucleic Acids Res. 16:7187-7187(1988) [PubMed: 3405767] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Mutational and crystallographic analyses of the active site residues of the Bacillus circulans xylanase." Wakarchuk W.W., Campbell R.L., Sung W.L., Davoodi J., Yaguchi M. Protein Sci. 3:467-475(1994) [PubMed: 8019418] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS), MUTAGENESIS.
[3]	"The pKa of the general acid/base carboxyl group of a glycosidase cycles during catalysis: a 13C-NMR study of Bacillus circulans xylanase." McIntosh L.P., Hand G., Johnson P.E., Joshi M.D., Koerner M., Plesniak L.A., Ziser L., Wakarchuk W.W., Withers S.G. Biochemistry 35:9958-9966(1996) [PubMed: 8756457] [Abstract] Cited for: STRUCTURE BY NMR.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X07723 Genomic DNA. Translation: CAA30553.1.

PIR

S01734.

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1BCX	X-ray	1.81	A	29-213	[»]
1BVV	X-ray	1.80	A	29-213	[»]
1C5H	X-ray	1.55	A	29-213	[»]
1C5I	X-ray	1.80	A	29-213	[»]
1HV0	X-ray	1.60	A	29-213	[»]
1HV1	X-ray	1.80	A	29-213	[»]
1XNB	X-ray	1.49	A	29-213	[»]
1XNC	X-ray	1.60	A	29-213	[»]
2BVV	X-ray	1.50	A	29-213	[»]

ModBase

Search...

Family and domain databases

InterPro

IPR001137. Glyco_hydro_11.
IPR013319. Glyco_hydro_11/12_cat.
[Graphical view]

Gene3D

G3DSA:2.60.120.180. Glyco_hydro_11/12_cat. 1 hit.

Pfam

PF00457. Glyco_hydro_11. 1 hit.
[Graphical view]

PRINTS

PR00911. GLHYDRLASE11.

PROSITE

PS00776. GLYCOSYL_HYDROL_F11_1. 1 hit.
PS00777. GLYCOSYL_HYDROL_F11_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

LinkHub

P09850.

Entry information

Entry name

XYNA_BACCI

Accession

Primary (citable) accession number: P09850

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	November 4, 2008
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)