Reviewed, UniProtKB/Swiss-Prot P0A078 (AMPM_STAAM)

Names and origin

Protein names

Recommended name:
    Methionine aminopeptidase
      Short name=MAP
    EC=3.4.11.18
Alternative name(s):
    Peptidase M

Gene names

Name:	map
Ordered Locus Names:	SAV1888

Organism

Staphylococcus aureus (strain Mu50 / ATCC 700699) [Complete proteome] [HAMAP]

Taxonomic identifier

158878 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	252 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Removes the amino-terminal methionine from nascent proteins By similarity.
Catalytic activity	Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.
Cofactor	Binds 2 cobalt ions per subunit By similarity.
Sequence similarities	Belongs to the peptidase M24A family.

Ontologies

Keywords
Ligand	Cobalt Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Molecule processing

Chain

1 – 252

252

Methionine aminopeptidase

Sites

Metal binding

93

1

Cobalt 2 By similarity

Metal binding

104

1

Cobalt 1 By similarity

Metal binding

104

1

Cobalt 2 By similarity

Metal binding

168

1

Cobalt 1 By similarity

Metal binding

202

1

Cobalt 1 By similarity

Metal binding

233

1

Cobalt 1 By similarity

Metal binding

233

1

Cobalt 2 By similarity

Secondary structure

1

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252

Helix Strand Turn

Details...

Helix

6 – 29

24

Helix

36 – 50

15

Helix

55 – 60

6

Beta strand

63 – 70

8

Beta strand

73 – 75

3

Beta strand

89 – 98

10

Beta strand

101 – 110

10

Helix

118 – 134

17

Helix

144 – 156

13

Beta strand

167 – 169

3

Beta strand

171 – 181

11

Beta strand

197 – 201

5

Beta strand

204 – 208

5

Beta strand

216 – 219

4

Beta strand

229 – 237

9

Sequences

	Sequence		Length	Mass (Da)
	P0A078-1 [UniParc]. Last modified March 1, 2005. Version 1. Checksum: 3E42E623286B537B	FASTA	252	27,502
	10 20 30 40 50 60 MIVKTEEELQ ALKEIGYICA KVRNTMQAAT KPGITTKELD NIAKELFEEY GAISAPIHDE 70 80 90 100 110 120 NFPGQTCISV NEEVAHGIPS KRVIREGDLV NIDVSALKNG YYADTGISFV VGESDDPMKQ 130 140 150 160 170 180 KVCDVATMAF ENAIAKVKPG TKLSNIGKAV HNTARQNDLK VIKNLTGHGV GLSLHEAPAH 190 200 210 220 230 240 VLNYFDPKDK TLLTEGMVLA IEPFISSNAS FVTEGKNEWA FETSDKSFVA QIEHTVIVTK 250 DGPILTTKIE EE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Whole genome sequencing of meticillin-resistant Staphylococcus aureus." Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L., Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M., Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y. , Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K., Hirakawa H., Kuhara S., Goto S., Yabuzaki J., Kanehisa M., Yamashita A., Oshima K., Furuya K., Yoshino C., Shiba T., Hattori M., Ogasawara N., Hayashi H., Hiramatsu K. Lancet 357:1225-1240(2001) [PubMed: 11418146] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Crystal structures of Staphylococcusaureus methionine aminopeptidase complexed with keto heterocycle and aminoketone inhibitors reveal the formation of a tetrahedral intermediate." Douangamath A., Dale G.E., D'Arcy A., Almstetter M., Eckl R., Frutos-Hoener A., Henkel B., Illgen K., Nerdinger S., Schulz H., Mac Sweeney A., Thormann M., Treml A., Pierau S., Wadman S., Oefner C. J. Med. Chem. 47:1325-1328(2004) [PubMed: 14998322] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.04 ANGSTROMS).

Cross-references

Sequence databases

EMBLGenBankDDBJ

BA000017 Genomic DNA. Translation: BAB58050.1.

RefSeq

NP_372412.1.

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1QXW	X-ray	1.67	A	1-252	[»]
1QXY	X-ray	1.04	A	1-252	[»]
1QXZ	X-ray	1.68	A	1-252	[»]

ModBase

Search...

2-D gel databases

World-2DPAGE

0002:P0A078.

Genome annotation databases

GeneID

1121901.

GenomeReviews

Gene locus SAV1888 in contig BA000017_GR.

KEGG

sav:SAV1888.

Organism-specific databases

CMR

Search...

Phylogenomic databases

HOGENOM

P0A078.

Enzyme and pathway databases

BioCyc

SAUR158878:SAV1888-MON.

Family and domain databases

InterPro

IPR001714. Pept_M24_MAP.
IPR002467. Pept_M24A_MAP1.
IPR000994. Peptidase_M24_cat_core.
[Graphical view]

Gene3D

G3DSA:3.90.230.10. Peptidase_M24_cat_core. 1 hit.

PANTHER

PTHR10804:SF13. Pept_M24A_MAP1. 1 hit.
PTHR10804. Peptidase_M24_cat_core. 1 hit.

Pfam

PF00557. Peptidase_M24. 1 hit.
[Graphical view]

PRINTS

PR00599. MAPEPTIDASE.

TIGRFAMs

TIGR00500. met_pdase_I. 1 hit.

ProDom

P0A078.
[Graphical view] [Entries sharing at least one domain]

BLOCKS

Search...

Other Resources

BindingDB

P0A078.

ProtoNet

Search...

Entry information

Entry name

AMPM_STAAM

Accession

Primary (citable) accession number: P0A078
Secondary accession number(s): Q9KWL1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	March 1, 2005
Last modified:	July 22, 2008
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)