Reviewed, UniProtKB/Swiss-Prot P0A462 (MTA1_ANAVA)

Names and origin

Protein names

Recommended name:
    Modification methylase AvaI
      Short name(s)=M.AvaI
    EC=2.1.1.113
Alternative name(s):
    N(4)- cytosine-specific methyltransferase AvaI

Gene names

Name:

avaIM

Organism

Anabaena variabilis

Taxonomic identifier

1172 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Anabaena

Protein attributes

Sequence length	482 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence CYCGRG, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the avaI endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA cytosine = S-adenosyl-L-homocysteine + DNA N(4)-methylcytosine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Molecular function	site-specific DNA-methyltransferase (cytosine-N4-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 482	482	Modification methylase AvaI

Sequences

	Sequence		Length	Mass (Da)
	P0A462-1 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 795ED0B445EA9E0C	FASTA	482	56,579
	10 20 30 40 50 60 MTSFELESPI EIKTDPTDLD QESDSFVQEI SRFNKALEQR FRDKMRLHES LSRKIVSFQA 70 80 90 100 110 120 NKSKPQYRWF KYKEAFSVDL VNQLIFEYEK KSFERILDPF AGAGTMLFAC SDAGIQADGI 130 140 150 160 170 180 EVLPIGQEII EVRKIIQRQF RREDFLRLIE WYKQKPWNQH NNRKYLNRLR ITDGAYPPET 190 200 210 220 230 240 EASIERFLFS IEKENILVKQ VLRFALLCIL ESISYTRKDG QYLRWDKRAF RKSGSDKFDK 250 260 270 280 290 300 GKILDFDEAI TEQIKLILND SFDLISNTLF CYGTQRSGIN LFNASCLKIL PEFEQDFYDC 310 320 330 340 350 360 IITSPPYCNR YDYTRTYALE LALLGVGERD IVQLRQDMLS CTVENKEKSL IHNWQEALRI 370 380 390 400 410 420 LDKQELLQSI LRFLERELER KKLNNNGIPR MIKGYFYEMA CVIIECFRVL KNGSPLFMVN 430 440 450 460 470 480 DNVRYAGIDI SVDLILSNIA EEIGFNVEKI LVLPTGKGNS SQQMGTHGRK TLRKCVYVWR KP

References

[1]

"Cloning and sequence comparison of AvaI and BsoBI restriction-modification systems." Ruan H., Lunnen K.D., Scott M.E., Moran L.S., Slatko B.E., Pelletier J.J., Hess E.J., Benner J. II, Wilson G.G., Xu S.-Y. Mol. Gen. Genet. 252:695-699(1996) [PubMed: 8917312] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: PCC 7118 / ATCC 27892.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X98339 Genomic DNA. Translation: CAA66984.1.
PIR	S72471.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	3287. M.AvaI.
Family and domain databases
InterPro	IPR001091. MeTrfase_CN4. IPR002296. N12N6_MeTrfase. [Graphical view]
PRINTS	PR00507. N12N6MTFRASE.
PROSITE	PS00093. N4_MTASE. 1 hit. [Graphical view]
ProDom	P0A462. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

MTA1_ANAVA

Accession

Primary (citable) accession number: P0A462
Secondary accession number(s): P70802

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	July 22, 2008
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)