Reviewed, UniProtKB/Swiss-Prot P0A5Y7 (INHA_MYCBO)

Names and origin

Protein names

Recommended name:
    Enoyl-[acyl-carrier-protein] reductase [NADH]
    EC=1.3.1.9
Alternative name(s):
    NADH-dependent enoyl-ACP reductase

Gene names

Name:	inhA
Ordered Locus Names:	Mb1520

Organism

Mycobacterium bovis [Complete proteome] [HAMAP]

Taxonomic identifier

1765 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	269 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in the resistance against the antituberculosis drugs isoniazid and ethionamide.
Catalytic activity	Acyl-[acyl-carrier-protein] + NAD(+) = trans-2,3-dehydroacyl-[acyl-carrier-protein] + NADH.
Pathway	Lipid metabolism; fatty acid biosynthesis. Context: Mycolic acid biosynthesis.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily.

Ontologies

Keywords
Biological process	Antibiotic resistance Fatty acid biosynthesis Lipid synthesis
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 269	269	Enoyl-[acyl-carrier-protein] reductase [NADH]
Regions
	Nucleotide binding	136 – 165	30	NAD Potential
Sites
	Binding site	158	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A5Y7-1 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: F161D6D6A631CA08	FASTA	269	28,528
	10 20 30 40 50 60 MTGLLDGKRI LVSGIITDSS IAFHIARVAQ EQGAQLVLTG FDRLRLIQRI TDRLPAKAPL 70 80 90 100 110 120 LELDVQNEEH LASLAGRVTE AIGAGNKLDG VVHSIGFMPQ TGMGINPFFD APYADVSKGI 130 140 150 160 170 180 HISAYSYASM AKALLPIMNP GGSIVGMDFD PSRAMPAYNW MTVAKSALES VNRFVAREAG 190 200 210 220 230 240 KYGVRSNLVA AGPIRTLAMS AIVGGALGEE AGAQIQLLEE GWDQRAPIGW NMKDATPVAK 250 260 TVCALLSDWL PATTGDIIYA DGGAHTQLL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"inhA, a gene encoding a target for isoniazid and ethionamide in Mycobacterium tuberculosis." Banerjee A., Dubnau E., Quemard A., Balasubramanian V., Um K.S., Wilson T., Collins D., de Lisle G., Jacobs W.R. Jr. Science 263:227-230(1994) [PubMed: 8284673] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: BCG.
[2]	"Effect of inhA and katG on isoniazid resistance and virulence of Mycobacterium bovis." Wilson T.M., de Lisle G.W., Collins D.M. Mol. Microbiol. 15:1009-1015(1995) [PubMed: 7623658] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: WAG201.
[3]	"The complete genome sequence of Mycobacterium bovis." Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M., Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B., Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J. , Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G. Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003) [PubMed: 12788972] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-935 / AF2122/97.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U41388 Genomic DNA. Translation: AAB60183.1. BX248339 Genomic DNA. Translation: CAD96187.1.
RefSeq	NP_855172.1.
3D structure databases
SMR	P0A5Y7. Positions 2-269.
ModBase	Search...
Genome annotation databases
GeneID	1092377.
GenomeReviews	Gene locus Mb1520 in contig BX248333_GR.
KEGG	mbo:Mb1520.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P0A5Y7.
Family and domain databases
InterPro	IPR002198. DHase_sc/Rdtase_SDR. IPR014358. Enoyl-ACP_rdct. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit. PTHR19410:SF12. Enoyl-ACP_rdct. 1 hit.
ProDom	P0A5Y7. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
BindingDB	P0A5Y7.
ProtoNet	Search...

Entry information

Entry name

INHA_MYCBO

Accession

Primary (citable) accession number: P0A5Y7
Secondary accession number(s): P46533

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	September 2, 2008
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)