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P0A8J8

- RHLB_ECOLI

UniProt

P0A8J8 - RHLB_ECOLI

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Protein
ATP-dependent RNA helicase RhlB
Gene
rhlB, mmrA, b3780, JW3753
Organism
Escherichia coli (strain K12)
Status
Reviewed - - Experimental evidence at protein leveli

Functioni

DEAD-box RNA helicase involved in RNA degradation. Has RNA-dependent ATPase activity and unwinds double-stranded RNA.3 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.2 Publications

Enzyme regulationi

ATPase activity is stimulated by interaction with RNase E.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier
Nucleotide bindingi53 – 608ATP By similarity

GO - Molecular functioni

  1. ATP bindingInferred from electronic annotationi Source: UniProtKB-HAMAP
  2. ATP-dependent RNA helicase activityInferred from electronic annotationi Source: UniProtKB-HAMAP
  3. ATP-dependent helicase activityInferred from direct assayi PubMed 8610017 Source: EcoCyc
  4. RNA bindingInferred from electronic annotationi Source: UniProtKB-HAMAP
  5. protein bindingInferred from physical interactioni PubMed 15236960PubMed 15690043PubMed 16139413PubMed 20729366PubMed 21126315 Source: IntAct
Complete GO annotation...

GO - Biological processi

  1. ATP catabolic processInferred from direct assayi PubMed 8610017 Source: GOC
  2. RNA catabolic processInferred from direct assayi PubMed 10521403 Source: EcoCyc
  1. ATP-dependent RNA helicase activityInferred from electronic annotationi Source: UniProtKB-HAMAP
  2. ATP-dependent helicase activityInferred from direct assayi PubMed 8610017 Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10844-MONOMER.
ECOL316407:JW3753-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase RhlB (EC:3.6.4.13)
Gene namesi
Name:rhlB
Synonyms:mmrA
Ordered Locus Names:b3780, JW3753
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10844. rhlB.

Subcellular locationi

Cytoplasm
Note: Forms a cytoskeletal-like coiled structure that extends along the length of the cell. Formation of this structure does not require the presence of RNase E, MinD and/or MreB.1 Publication

GO - Cellular componenti

  1. cytoplasmInferred from electronic annotationi Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 421420ATP-dependent RNA helicase RhlBUniRule annotation
PRO_0000200769

Proteomic databases

PaxDbiP0A8J8.
PRIDEiP0A8J8.

Expressioni

Gene expression databases

GenevestigatoriP0A8J8.

Interactioni

Subunit structurei

Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation. Binds to RNase E (rne) and PNPase (pnp). Forms multimers.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
pnpP050556EBI-555806,EBI-548080
rneP2151316EBI-555806,EBI-549958
rneQ1ZS714EBI-555806,EBI-8525650From a different organism.

Protein-protein interaction databases

DIPiDIP-35644N.
IntActiP0A8J8. 32 interactions.
MINTiMINT-8049290.
STRINGi511145.b3780.

Structurei

3D structure databases

ProteinModelPortaliP0A8J8.
SMRiP0A8J8. Positions 9-379.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier
Domaini40 – 219180Helicase ATP-binding
Domaini245 – 390146Helicase C-terminal

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier
Motifi9 – 3729Q motifUniRule annotation
Motifi165 – 1684DEAD boxUniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0513.
HOGENOMiHOG000268807.
KOiK03732.
OMAiPGRIIDY.
OrthoDBiEOG6GBMBM.
PhylomeDBiP0A8J8.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_00661. DEAD_helicase_RhlB.
InterProiIPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR023554. RNA_helicase_ATP-dep_RhlB.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A8J8-1 [UniParc]FASTA

« Hide

MSKTHLTEQK FSDFALHPKV VEALEKKGFH NCTPIQALAL PLTLAGRDVA    50
GQAQTGTGKT MAFLTSTFHY LLSHPAIADR KVNQPRALIM APTRELAVQI 100
HADAEPLAEA TGLKLGLAYG GDGYDKQLKV LESGVDILIG TTGRLIDYAK 150
QNHINLGAIQ VVVLDEADRM YDLGFIKDIR WLFRRMPPAN QRLNMLFSAT 200
LSYRVRELAF EQMNNAEYIE VEPEQKTGHR IKEELFYPSN EEKMRLLQTL 250
IEEEWPDRAI IFANTKHRCE EIWGHLAADG HRVGLLTGDV AQKKRLRILD 300
EFTRGDLDIL VATDVAARGL HIPAVTHVFN YDLPDDCEDY VHRIGRTGRA 350
GASGHSISLA CEEYALNLPA IETYIGHSIP VSKYNPDALM TDLPKPLRLT 400
RPRTGNGPRR TGAPRNRRRS G 421
Length:421
Mass (Da):47,126
Last modified:January 23, 2007 - v2
Checksum:i48E1ADD025CBFA1A
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier
Sequence conflicti72 – 721L → H in X56310. 1 Publication
Sequence conflicti340 – 3401Y → S in X56310. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X56310 Genomic DNA. No translation available.
M87049 Genomic DNA. Translation: AAA67581.1.
M83316 Genomic DNA. Translation: AAB59048.1.
U00096 Genomic DNA. Translation: AAC76785.1.
AP009048 Genomic DNA. Translation: BAE77518.1.
PIRiG65181.
RefSeqiNP_418227.1. NC_000913.3.
YP_491659.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC76785; AAC76785; b3780.
BAE77518; BAE77518; BAE77518.
GeneIDi12934326.
948290.
KEGGiecj:Y75_p3395.
eco:b3780.
PATRICi32123051. VBIEscCol129921_3895.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X56310 Genomic DNA. No translation available.
M87049 Genomic DNA. Translation: AAA67581.1 .
M83316 Genomic DNA. Translation: AAB59048.1 .
U00096 Genomic DNA. Translation: AAC76785.1 .
AP009048 Genomic DNA. Translation: BAE77518.1 .
PIRi G65181.
RefSeqi NP_418227.1. NC_000913.3.
YP_491659.1. NC_007779.1.

3D structure databases

ProteinModelPortali P0A8J8.
SMRi P0A8J8. Positions 9-379.
ModBasei Search...

Protein-protein interaction databases

DIPi DIP-35644N.
IntActi P0A8J8. 32 interactions.
MINTi MINT-8049290.
STRINGi 511145.b3780.

Proteomic databases

PaxDbi P0A8J8.
PRIDEi P0A8J8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC76785 ; AAC76785 ; b3780 .
BAE77518 ; BAE77518 ; BAE77518 .
GeneIDi 12934326.
948290.
KEGGi ecj:Y75_p3395.
eco:b3780.
PATRICi 32123051. VBIEscCol129921_3895.

Organism-specific databases

EchoBASEi EB0837.
EcoGenei EG10844. rhlB.

Phylogenomic databases

eggNOGi COG0513.
HOGENOMi HOG000268807.
KOi K03732.
OMAi PGRIIDY.
OrthoDBi EOG6GBMBM.
PhylomeDBi P0A8J8.

Enzyme and pathway databases

BioCyci EcoCyc:EG10844-MONOMER.
ECOL316407:JW3753-MONOMER.

Miscellaneous databases

PROi P0A8J8.

Gene expression databases

Genevestigatori P0A8J8.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
HAMAPi MF_00661. DEAD_helicase_RhlB.
InterProi IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR023554. RNA_helicase_ATP-dep_RhlB.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view ]
Pfami PF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view ]
SMARTi SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
PROSITEi PS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publicationsDownload
  1. "rhlB, a new Escherichia coli K-12 gene with an RNA helicase-like protein sequence motif, one of at least five such possible genes in a prokaryote."
    Kalman M., Murphy H., Cashel M.
    New Biol. 3:886-895(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes."
    Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R.
    Science 257:771-778(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "The complete genome sequence of Escherichia coli K-12."
    Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
    Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "A DEAD-box RNA helicase in the Escherichia coli RNA degradosome."
    Py B., Higgins C.F., Krisch H.M., Carpousis A.J.
    Nature 381:169-172(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-16, FUNCTION, CATALYTIC ACTIVITY, SUBUNIT.
  6. "Ribonuclease E organizes the protein interactions in the Escherichia coli RNA degradosome."
    Vanzo N.F., Li Y.S., Py B., Blum E., Higgins C.F., Raynal L.C., Krisch H.M., Carpousis A.J.
    Genes Dev. 12:2770-2781(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, INTERACTION WITH RNASE E, SUBUNIT.
  7. "Reconstitution of a minimal RNA degradosome demonstrates functional coordination between a 3' exonuclease and a DEAD-box RNA helicase."
    Coburn G.A., Miao X., Briant D.J., Mackie G.A.
    Genes Dev. 13:2594-2603(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RNASE E, SUBUNIT.
  8. "DEAD box RhlB RNA helicase physically associates with exoribonuclease PNPase to degrade double-stranded RNA independent of the degradosome-assembling region of RNase E."
    Liou G.-G., Chang H.-Y., Lin C.-S., Lin-Chao S.
    J. Biol. Chem. 277:41157-41162(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PNPASE AND RNASE E.
  9. "RNaseE and RNA helicase B play central roles in the cytoskeletal organization of the RNA degradosome."
    Taghbalout A., Rothfield L.
    J. Biol. Chem. 283:13850-13855(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, SUBUNIT.

Entry nameiRHLB_ECOLI
AccessioniPrimary (citable) accession number: P0A8J8
Secondary accession number(s): P24229, Q2M888
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene

External Data

Dasty 3

Similar proteinsi