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P0A964

- CHEW_ECOLI

UniProt

P0A964 - CHEW_ECOLI

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Protein
Chemotaxis protein CheW
Gene
cheW, b1887, JW1876
Organism
Escherichia coli (strain K12)
Status
Reviewed - - Experimental evidence at protein leveli

Functioni

Involved in the transmission of sensory signals from the chemoreceptors to the flagellar motors. It physically bridges CheA to the MCPs (methyl-accepting chemotaxis proteins) to allow regulated phosphotransfer to CheY and CheB.

GO - Molecular functioni

  1. protein bindingInferred from physical interactioni PubMed 16606699PubMed 19834901 Source: IntAct
  2. signal transducer activityInferred from electronic annotationi Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. chemotaxisInferred from mutant phenotypei PubMed 2682657 Source: EcoCyc
  2. establishment of localization in cellInferred from mutant phenotypei PubMed 16856941 Source: CACAO
  3. signal transductionInferred from direct assayi PubMed 1992467 Source: EcoCyc
  1. signal transducer activityInferred from electronic annotationi Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Chemotaxis

Enzyme and pathway databases

BioCyciEcoCyc:CHEW-MONOMER.
ECOL316407:JW1876-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Chemotaxis protein CheW
Gene namesi
Name:cheW
Ordered Locus Names:b1887, JW1876
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10149. cheW.

Subcellular locationi

GO - Cellular componenti

  1. cytosolInferred from direct assayi PubMed 334749 Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier
Chaini1 – 167167Chemotaxis protein CheW
PRO_0000198341

Proteomic databases

PaxDbiP0A964.

Expressioni

Gene expression databases

GenevestigatoriP0A964.

Interactioni

Subunit structurei

An in vitro complex of CheW/CheA(L)/CheA(S) in a 1:1:1 ratio increases the autophosphorylation of CheA and is required for the binding of CheY, the phosphorylation substrate. This complex accounts for 10% of the total number of molecules.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
tarP070173EBI-1125947,EBI-1125130

Protein-protein interaction databases

DIPiDIP-48236N.
IntActiP0A964. 31 interactions.
MINTiMINT-1283783.
STRINGi511145.b1887.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier
Beta strandi10 – 134
Beta strandi17 – 248
Beta strandi27 – 326
Helixi33 – 353
Beta strandi36 – 427
Beta strandi55 – 617
Beta strandi64 – 696
Helixi71 – 755
Beta strandi87 – 937
Beta strandi96 – 11116
Turni113 – 1153
Beta strandi122 – 1254
Helixi128 – 1303
Beta strandi133 – 1375
Beta strandi140 – 1445
Helixi147 – 15812
Helixi159 – 1613

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2HO9NMR-A1-167[»]
ProteinModelPortaliP0A964.
SMRiP0A964. Positions 1-167.

Miscellaneous databases

EvolutionaryTraceiP0A964.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier
Domaini16 – 156141CheW-like

Sequence similaritiesi

Contains 1 cheW-like domain.

Phylogenomic databases

eggNOGiCOG0835.
HOGENOMiHOG000254712.
KOiK03408.
OMAiVTYNDNT.
OrthoDBiEOG60CWQ7.
PhylomeDBiP0A964.

Family and domain databases

InterProiIPR002545. CheW.
[Graphical view]
PfamiPF01584. CheW. 1 hit.
[Graphical view]
SMARTiSM00260. CheW. 1 hit.
[Graphical view]
SUPFAMiSSF50341. SSF50341. 1 hit.
PROSITEiPS50851. CHEW. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A964-1 [UniParc]FASTA

« Hide

MTGMTNVTKL ASEPSGQEFL VFTLGDEEYG IDILKVQEIR GYDQVTRIAN    50
TPAFIKGVTN LRGVIVPIVD LRIKFSQVDV DYNDNTVVIV LNLGQRVVGI 100
VVDGVSDVLS LTAEQIRPAP EFAVTLSTEY LTGLGALGDR MLILVNIEKL 150
LNSEEMALLD SAASEVA 167
Length:167
Mass (Da):18,084
Last modified:April 1, 1988 - v1
Checksum:i9CE642E5F4E91BFF
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier
Sequence conflicti16 – 161G → R in AAA23575. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M13462 Genomic DNA. Translation: AAA23565.1.
U00096 Genomic DNA. Translation: AAC74957.1.
AP009048 Genomic DNA. Translation: BAA15703.1.
M34669 Genomic DNA. Translation: AAA23575.1.
PIRiQRECCW. B25195.
RefSeqiNP_416401.1. NC_000913.3.
YP_490149.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC74957; AAC74957; b1887.
BAA15703; BAA15703; BAA15703.
GeneIDi12932044.
946400.
KEGGiecj:Y75_p1863.
eco:b1887.
PATRICi32119101. VBIEscCol129921_1968.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M13462 Genomic DNA. Translation: AAA23565.1 .
U00096 Genomic DNA. Translation: AAC74957.1 .
AP009048 Genomic DNA. Translation: BAA15703.1 .
M34669 Genomic DNA. Translation: AAA23575.1 .
PIRi QRECCW. B25195.
RefSeqi NP_416401.1. NC_000913.3.
YP_490149.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2HO9 NMR - A 1-167 [» ]
ProteinModelPortali P0A964.
SMRi P0A964. Positions 1-167.
ModBasei Search...

Protein-protein interaction databases

DIPi DIP-48236N.
IntActi P0A964. 31 interactions.
MINTi MINT-1283783.
STRINGi 511145.b1887.

Proteomic databases

PaxDbi P0A964.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC74957 ; AAC74957 ; b1887 .
BAA15703 ; BAA15703 ; BAA15703 .
GeneIDi 12932044.
946400.
KEGGi ecj:Y75_p1863.
eco:b1887.
PATRICi 32119101. VBIEscCol129921_1968.

Organism-specific databases

EchoBASEi EB0147.
EcoGenei EG10149. cheW.

Phylogenomic databases

eggNOGi COG0835.
HOGENOMi HOG000254712.
KOi K03408.
OMAi VTYNDNT.
OrthoDBi EOG60CWQ7.
PhylomeDBi P0A964.

Enzyme and pathway databases

BioCyci EcoCyc:CHEW-MONOMER.
ECOL316407:JW1876-MONOMER.

Miscellaneous databases

EvolutionaryTracei P0A964.
PROi P0A964.

Gene expression databases

Genevestigatori P0A964.

Family and domain databases

InterProi IPR002545. CheW.
[Graphical view ]
Pfami PF01584. CheW. 1 hit.
[Graphical view ]
SMARTi SM00260. CheW. 1 hit.
[Graphical view ]
SUPFAMi SSF50341. SSF50341. 1 hit.
PROSITEi PS50851. CHEW. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publicationsDownload
  1. "Nucleotide sequence corresponding to five chemotaxis genes in Escherichia coli."
    Mutoh N., Simon M.I.
    J. Bacteriol. 165:161-166(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map."
    Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S.
    , Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T.
    DNA Res. 3:379-392(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "The complete genome sequence of Escherichia coli K-12."
    Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
    Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Tandem translation starts in the cheA locus of Escherichia coli."
    Kofoid E.C., Parkinson J.S.
    J. Bacteriol. 173:2116-2119(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-32.
  6. "Bacterial chemotaxis signaling complexes: formation of a CheA/CheW complex enhances autophosphorylation and affinity for CheY."
    McNally D.F., Matsumura P.
    Proc. Natl. Acad. Sci. U.S.A. 88:6269-6273(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.

Entry nameiCHEW_ECOLI
AccessioniPrimary (citable) accession number: P0A964
Secondary accession number(s): P07365
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: June 11, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene

External Data

Dasty 3

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