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P0A9Q1

- ARCA_ECOLI

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Protein
Aerobic respiration control protein ArcA
Gene
arcA, cpxC, dye, fexA, msp, seg, sfrA, b4401, JW4364

Gene:

arcA, cpxC, dye, fexA, msp, seg, sfrA, b4401, JW4364
Organism
Escherichia coli (strain K12)
Status
Reviewed - - Experimental evidence at protein leveli

UniProt

P0A9Q1 - ARCA_ECOLI

Protein:

Aerobic respiration control protein ArcA
Protein Existence: Experimental evidence at protein level

Functioni

Member of the two-component regulatory system ArcB/ArcA. Represses a wide variety of aerobic enzymes under anaerobic conditions. Controls the resistance of E.coli to dyes; required for expression of the alkaline phosphatase and sex factor F genes; It also may be involved in the osmoregulation of envelope proteins. When activated by ArcB, it negatively regulates the expression of genes of aerobic function. Activates the transcription of the plfB operon by binding to its promoter.

GO - Molecular functioni

  1. DNA bindingInferred from electronic annotationi Source: UniProtKB-KW
  2. identical protein bindingInferred from physical interactioni PubMed 18631241 Source: IntAct
  3. phosphorelay response regulator activityInferred from direct assayi PubMed 1512197 Source: EcoCyc
  4. sequence-specific DNA binding transcription factor activityInferred from direct assayi PubMed 14711822PubMed 2964639 Source: EcoCyc
Complete GO annotation...

GO - Biological processi

  1. DNA bindingInferred from electronic annotationi Source: UniProtKB-KW
  2. identical protein bindingInferred from physical interactioni PubMed 18631241 Source: IntAct
  3. phosphorelay response regulator activityInferred from direct assayi PubMed 1512197 Source: EcoCyc
  4. sequence-specific DNA binding transcription factor activityInferred from direct assayi PubMed 14711822PubMed 2964639 Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Transcription, Transcription regulation, Two-component regulatory system

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:ARCA-MONOMER.
ECOL316407:JW4364-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Aerobic respiration control protein ArcA
Alternative name(s):
Dye resistance protein
Gene namesi
Name:arcA
Synonyms:cpxC, dye, fexA, msp, seg, sfrA
Ordered Locus Names:b4401, JW4364
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10061. arcA.

Subcellular locationi

Cytoplasm Inferred

GO - Cellular componenti

  1. cytoplasmInferred from electronic annotationi Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier
Chaini1 – 238238Aerobic respiration control protein ArcA
PRO_0000081008

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier
Modified residuei5414-aspartylphosphate By similarity

Post-translational modificationi

Phosphorylated by ArcB.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP0A9Q1.
PRIDEiP0A9Q1.

2D gel databases

SWISS-2DPAGEiP0A9Q1.

Expressioni

Gene expression databases

GenevestigatoriP0A9Q1.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-1119939,EBI-1119939
mexBP520022EBI-1119939,EBI-6400435From a different organism.

Protein-protein interaction databases

DIPiDIP-36035N.
IntActiP0A9Q1. 7 interactions.
STRINGi511145.b4401.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier
Beta strandi5 – 95
Helixi13 – 2412
Turni25 – 273
Beta strandi29 – 357
Helixi36 – 4510
Beta strandi49 – 535
Beta strandi58 – 603
Helixi62 – 7211
Beta strandi76 – 827
Helixi86 – 9510
Beta strandi98 – 1047
Helixi107 – 12115

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1XHEX-ray2.50A/B1-122[»]
1XHFX-ray2.15A/B1-122[»]
ProteinModelPortaliP0A9Q1.
SMRiP0A9Q1. Positions 1-234.

Miscellaneous databases

EvolutionaryTraceiP0A9Q1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier
Domaini1 – 118118Response regulatory

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0745.
HOGENOMiHOG000034819.
KOiK07773.
OMAiENTMSLA.
OrthoDBiEOG6G4VQG.
PhylomeDBiP0A9Q1.
ProtClustDBiPRK11173.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR001867. Sig_transdc_resp-reg_C.
IPR016032. Sig_transdc_resp-reg_C-effctor.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00072. Response_reg. 1 hit.
PF00486. Trans_reg_C. 1 hit.
[Graphical view]
SMARTiSM00448. REC. 1 hit.
SM00862. Trans_reg_C. 1 hit.
[Graphical view]
SUPFAMiSSF46894. SSF46894. 1 hit.
SSF52172. SSF52172. 1 hit.
PROSITEiPS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A9Q1-1 [UniParc]FASTA

« Hide

MQTPHILIVE DELVTRNTLK SIFEAEGYDV FEATDGAEMH QILSEYDINL    50
VIMDINLPGK NGLLLARELR EQANVALMFL TGRDNEVDKI LGLEIGADDY 100
ITKPFNPREL TIRARNLLSR TMNLGTVSEE RRSVESYKFN GWELDINSRS 150
LIGPDGEQYK LPRSEFRAML HFCENPGKIQ SRAELLKKMT GRELKPHDRT 200
VDVTIRRIRK HFESTPDTPE IIATIHGEGY RFCGDLED 238
Length:238
Mass (Da):27,292
Last modified:July 21, 1986 - v1
Checksum:i9316CF4DE8EABDE8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M10044 Genomic DNA. Translation: AAA23718.1.
U14003 Genomic DNA. Translation: AAA97297.1.
U00096 Genomic DNA. Translation: AAC77354.1.
AP009048 Genomic DNA. Translation: BAE78390.1.
L34010 Genomic DNA. Translation: AAA23476.1.
PIRiJYECR. A03561.
RefSeqiNP_418818.1. NC_000913.3.
YP_492531.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC77354; AAC77354; b4401.
BAE78390; BAE78390; BAE78390.
GeneIDi12930392.
948874.
KEGGiecj:Y75_p4285.
eco:b4401.
PATRICi32124420. VBIEscCol129921_4550.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M10044 Genomic DNA. Translation: AAA23718.1 .
U14003 Genomic DNA. Translation: AAA97297.1 .
U00096 Genomic DNA. Translation: AAC77354.1 .
AP009048 Genomic DNA. Translation: BAE78390.1 .
L34010 Genomic DNA. Translation: AAA23476.1 .
PIRi JYECR. A03561.
RefSeqi NP_418818.1. NC_000913.3.
YP_492531.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1XHE X-ray 2.50 A/B 1-122 [» ]
1XHF X-ray 2.15 A/B 1-122 [» ]
ProteinModelPortali P0A9Q1.
SMRi P0A9Q1. Positions 1-234.
ModBasei Search...

Protein-protein interaction databases

DIPi DIP-36035N.
IntActi P0A9Q1. 7 interactions.
STRINGi 511145.b4401.

2D gel databases

SWISS-2DPAGEi P0A9Q1.

Proteomic databases

PaxDbi P0A9Q1.
PRIDEi P0A9Q1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC77354 ; AAC77354 ; b4401 .
BAE78390 ; BAE78390 ; BAE78390 .
GeneIDi 12930392.
948874.
KEGGi ecj:Y75_p4285.
eco:b4401.
PATRICi 32124420. VBIEscCol129921_4550.

Organism-specific databases

EchoBASEi EB0059.
EcoGenei EG10061. arcA.

Phylogenomic databases

eggNOGi COG0745.
HOGENOMi HOG000034819.
KOi K07773.
OMAi ENTMSLA.
OrthoDBi EOG6G4VQG.
PhylomeDBi P0A9Q1.
ProtClustDBi PRK11173.

Enzyme and pathway databases

BioCyci EcoCyc:ARCA-MONOMER.
ECOL316407:JW4364-MONOMER.

Miscellaneous databases

EvolutionaryTracei P0A9Q1.
PROi P0A9Q1.

Gene expression databases

Genevestigatori P0A9Q1.

Family and domain databases

Gene3Di 1.10.10.10. 1 hit.
InterProi IPR011006. CheY-like_superfamily.
IPR001867. Sig_transdc_resp-reg_C.
IPR016032. Sig_transdc_resp-reg_C-effctor.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view ]
Pfami PF00072. Response_reg. 1 hit.
PF00486. Trans_reg_C. 1 hit.
[Graphical view ]
SMARTi SM00448. REC. 1 hit.
SM00862. Trans_reg_C. 1 hit.
[Graphical view ]
SUPFAMi SSF46894. SSF46894. 1 hit.
SSF52172. SSF52172. 1 hit.
PROSITEi PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publicationsDownload
  1. "DNA sequence analysis of the dye gene of Escherichia coli reveals amino acid homology between the dye and OmpR proteins."
    Drury L.S., Buxton R.S.
    J. Biol. Chem. 260:4236-4242(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
    Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
    Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "The complete genome sequence of Escherichia coli K-12."
    Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
    Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. Park S.J., Gunsalus R.P.
    Submitted (JUN-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-23.
    Strain: K12.
  6. "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12."
    Link A.J., Robison K., Church G.M.
    Electrophoresis 18:1259-1313(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-12.
    Strain: K12 / EMG2.
  7. "Purification of ArcA and analysis of its specific interaction with the pfl promoter-regulatory region."
    Drapal N., Sawers G.
    Mol. Microbiol. 16:597-607(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 40-45 AND 123-128, CHARACTERIZATION.

Entry nameiARCA_ECOLI
AccessioniPrimary (citable) accession number: P0A9Q1
Secondary accession number(s): P03026, Q2M5R6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: April 16, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene

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