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P0AEC3

- ARCB_ECOLI

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Protein
Aerobic respiration control sensor protein ArcB
Gene
arcB, b3210, JW5536

Gene:

arcB, b3210, JW5536
Organism
Escherichia coli (strain K12)
Status
Reviewed - - Experimental evidence at protein leveli

UniProt

P0AEC3 - ARCB_ECOLI

Protein:

Aerobic respiration control sensor protein ArcB
Protein Existence: Experimental evidence at protein level

Functioni

Member of the two-component regulatory system ArcB/ArcA. Sensor-regulator protein for anaerobic repression of the arc modulon. Activates ArcA via a four-step phosphorelay. ArcB can also dephosphorylate ArcA by a reverse phosphorelay involving His-717 and Asp-576.

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

GO - Molecular functioni

  1. ATP bindingInferred from electronic annotationi Source: UniProtKB-KW
  2. histidine phosphotransfer kinase activityInferred from direct assayi PubMed 1512197 Source: EcoCyc
  3. phosphorelay response regulator activityInferred from electronic annotationi Source: InterPro
  4. phosphorelay sensor kinase activityInferred from direct assayi PubMed 1512197 Source: EcoCyc
Complete GO annotation...

GO - Biological processi

  1. ATP bindingInferred from electronic annotationi Source: UniProtKB-KW
  2. histidine phosphotransfer kinase activityInferred from direct assayi PubMed 1512197 Source: EcoCyc
  3. phosphorelay response regulator activityInferred from electronic annotationi Source: InterPro
  4. phosphorelay sensor kinase activityInferred from direct assayi PubMed 1512197 Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Transcription, Transcription regulation, Two-component regulatory system

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:ARCB-MONOMER.
ECOL316407:JW5536-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Aerobic respiration control sensor protein ArcB (EC:2.7.13.3)
Gene namesi
Name:arcB
Ordered Locus Names:b3210, JW5536
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10062. arcB.

Subcellular locationi

Cell inner membrane; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier
Topological domaini1 – 2525Cytoplasmic Reviewed prediction
Transmembranei26 – 4621Helical; Reviewed prediction
Topological domaini47 – 5711Periplasmic Reviewed prediction
Transmembranei58 – 7821Helical; Reviewed prediction
Topological domaini79 – 778700Cytoplasmic Reviewed prediction

GO - Cellular componenti

  1. integral component of membraneInferred from direct assayi PubMed 2201868 Source: EcoCyc
  2. plasma membraneInferred from electronic annotationi Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier
Mutagenesisi2921H → Q: Loss of activity. 1 Publication
Mutagenesisi5761D → A: Loss of activity. 1 Publication
Mutagenesisi7171H → Q: Loss of activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier
Chaini1 – 778778Aerobic respiration control sensor protein ArcB
PRO_0000074684

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier
Modified residuei2921Phosphohistidine; by autocatalysis
Modified residuei57614-aspartylphosphate Inferred
Modified residuei7171Phosphohistidine Inferred

Post-translational modificationi

Activation requires a sequential transfer of a phosphate group from a His in the primary transmitter domain, to an Asp in the receiver domain and to a His in the secondary transmitter domain.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP0AEC3.
PRIDEiP0AEC3.

Expressioni

Gene expression databases

GenevestigatoriP0AEC3.

Interactioni

Protein-protein interaction databases

DIPiDIP-47915N.
IntActiP0AEC3. 5 interactions.
MINTiMINT-98513.
STRINGi511145.b3210.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier
Helixi27 – 4519
Beta strandi50 – 545
Turni58 – 603
Helixi61 – 7818
Helixi660 – 6645
Helixi667 – 67610
Helixi679 – 70527
Helixi709 – 72517
Helixi729 – 73810
Helixi746 – 77530

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1A0BX-ray2.06A654-778[»]
1BDJX-ray2.68B654-778[»]
1FR0NMR-A654-778[»]
2A0BX-ray1.57A654-778[»]
2KSDNMR-A1-115[»]
ProteinModelPortaliP0AEC3.
SMRiP0AEC3. Positions 15-89, 144-505, 528-646, 659-776.

Miscellaneous databases

EvolutionaryTraceiP0AEC3.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier
Domaini153 – 22371PAS
Domaini226 – 27853PAC
Domaini289 – 507219Histidine kinase
Domaini527 – 643117Response regulatory
Domaini678 – 77194HPt

Sequence similaritiesi

Contains 1 HPt domain.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0642.
HOGENOMiHOG000272667.
KOiK07648.
OMAiESRNLEN.
OrthoDBiEOG6G4VQG.
ProtClustDBiPRK11091.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
1.10.287.970. 1 hit.
1.20.120.160. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR027460. ArcB_TM.
IPR011006. CheY-like_superfamily.
IPR003661. EnvZ-like_dim/P.
IPR003594. HATPase_ATP-bd.
IPR000014. PAS.
IPR000700. PAS-assoc_C.
IPR013767. PAS_fold.
IPR004358. Sig_transdc_His_kin-like_C.
IPR008207. Sig_transdc_His_kin_Hpt_dom.
IPR014409. Sig_transdc_His_kin_hyb_ArcB.
IPR005467. Sig_transdc_His_kinase_core.
IPR009082. Sig_transdc_His_kinase_dimeric.
IPR001789. Sig_transdc_resp-reg_receiver.
[Graphical view]
PfamiPF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF01627. Hpt. 1 hit.
PF00989. PAS. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PIRSFiPIRSF003182. ArcB. 1 hit.
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00073. HPT. 1 hit.
SM00091. PAS. 1 hit.
SM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF47226. SSF47226. 1 hit.
SSF47384. SSF47384. 1 hit.
SSF52172. SSF52172. 1 hit.
SSF55785. SSF55785. 1 hit.
SSF55874. SSF55874. 1 hit.
TIGRFAMsiTIGR00229. sensory_box. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
PS50894. HPT. 1 hit.
PS50113. PAC. 1 hit.
PS50112. PAS. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AEC3-1 [UniParc]FASTA

« Hide

MKQIRLLAQY YVDLMMKLGL VRFSMLLALA LVVLAIVVQM AVTMVLHGQV    50
ESIDVIRSIF FGLLITPWAV YFLSVVVEQL EESRQRLSRL VQKLEEMRER 100
DLSLNVQLKD NIAQLNQEIA VREKAEAELQ ETFGQLKIEI KEREETQIQL 150
EQQSSFLRSF LDASPDLVFY RNEDKEFSGC NRAMELLTGK SEKQLVHLKP 200
ADVYSPEAAA KVIETDEKVF RHNVSLTYEQ WLDYPDGRKA CFEIRKVPYY 250
DRVGKRHGLM GFGRDITERK RYQDALERAS RDKTTFISTI SHELRTPLNG 300
IVGLSRILLD TELTAEQEKY LKTIHVSAVT LGNIFNDIID MDKMERRKVQ 350
LDNQPVDFTS FLADLENLSA LQAQQKGLRF NLEPTLPLPH QVITDGTRLR 400
QILWNLISNA VKFTQQGQVT VRVRYDEGDM LHFEVEDSGI GIPQDELDKI 450
FAMYYQVKDS HGGKPATGTG IGLAVSRRLA KNMGGDITVT SEQGKGSTFT 500
LTIHAPSVAE EVDDAFDEDD MPLPALNVLL VEDIELNVIV ARSVLEKLGN 550
SVDVAMTGKA ALEMFKPGEY DLVLLDIQLP DMTGLDISRE LTKRYPREDL 600
PPLVALTANV LKDKQEYLNA GMDDVLSKPL SVPALTAMIK KFWDTQDDEE 650
STVTTEENSK SEALLDIPML EQYLELVGPK LITDGLAVFE KMMPGYVSVL 700
ESNLTAQDKK GIVEEGHKIK GAAGSVGLRH LQQLGQQIQS PDLPAWEDNV 750
GEWIEEMKEE WRHDVEVLKA WVAKATKK 778
Length:778
Mass (Da):87,983
Last modified:December 6, 2005 - v1
Checksum:iDD61EA6ECF95AD30
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier
Sequence conflicti469 – 4702Missing in AAA58012. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X53315 Genomic DNA. Translation: CAA37397.1.
U18997 Genomic DNA. Translation: AAA58012.1.
U00096 Genomic DNA. Translation: AAT48172.1.
AP009048 Genomic DNA. Translation: BAE77254.1.
PIRiRGECAR. D65112.
RefSeqiYP_026207.1. NC_000913.3.
YP_491395.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAT48172; AAT48172; b3210.
BAE77254; BAE77254; BAE77254.
GeneIDi12934428.
947887.
KEGGiecj:Y75_p3130.
eco:b3210.
PATRICi32121840. VBIEscCol129921_3304.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X53315 Genomic DNA. Translation: CAA37397.1 .
U18997 Genomic DNA. Translation: AAA58012.1 .
U00096 Genomic DNA. Translation: AAT48172.1 .
AP009048 Genomic DNA. Translation: BAE77254.1 .
PIRi RGECAR. D65112.
RefSeqi YP_026207.1. NC_000913.3.
YP_491395.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1A0B X-ray 2.06 A 654-778 [» ]
1BDJ X-ray 2.68 B 654-778 [» ]
1FR0 NMR - A 654-778 [» ]
2A0B X-ray 1.57 A 654-778 [» ]
2KSD NMR - A 1-115 [» ]
ProteinModelPortali P0AEC3.
SMRi P0AEC3. Positions 15-89, 144-505, 528-646, 659-776.
ModBasei Search...

Protein-protein interaction databases

DIPi DIP-47915N.
IntActi P0AEC3. 5 interactions.
MINTi MINT-98513.
STRINGi 511145.b3210.

Proteomic databases

PaxDbi P0AEC3.
PRIDEi P0AEC3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAT48172 ; AAT48172 ; b3210 .
BAE77254 ; BAE77254 ; BAE77254 .
GeneIDi 12934428.
947887.
KEGGi ecj:Y75_p3130.
eco:b3210.
PATRICi 32121840. VBIEscCol129921_3304.

Organism-specific databases

EchoBASEi EB0060.
EcoGenei EG10062. arcB.

Phylogenomic databases

eggNOGi COG0642.
HOGENOMi HOG000272667.
KOi K07648.
OMAi ESRNLEN.
OrthoDBi EOG6G4VQG.
ProtClustDBi PRK11091.

Enzyme and pathway databases

BioCyci EcoCyc:ARCB-MONOMER.
ECOL316407:JW5536-MONOMER.

Miscellaneous databases

EvolutionaryTracei P0AEC3.
PROi P0AEC3.

Gene expression databases

Genevestigatori P0AEC3.

Family and domain databases

Gene3Di 1.10.287.130. 1 hit.
1.10.287.970. 1 hit.
1.20.120.160. 1 hit.
3.30.565.10. 1 hit.
InterProi IPR027460. ArcB_TM.
IPR011006. CheY-like_superfamily.
IPR003661. EnvZ-like_dim/P.
IPR003594. HATPase_ATP-bd.
IPR000014. PAS.
IPR000700. PAS-assoc_C.
IPR013767. PAS_fold.
IPR004358. Sig_transdc_His_kin-like_C.
IPR008207. Sig_transdc_His_kin_Hpt_dom.
IPR014409. Sig_transdc_His_kin_hyb_ArcB.
IPR005467. Sig_transdc_His_kinase_core.
IPR009082. Sig_transdc_His_kinase_dimeric.
IPR001789. Sig_transdc_resp-reg_receiver.
[Graphical view ]
Pfami PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF01627. Hpt. 1 hit.
PF00989. PAS. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view ]
PIRSFi PIRSF003182. ArcB. 1 hit.
PRINTSi PR00344. BCTRLSENSOR.
SMARTi SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00073. HPT. 1 hit.
SM00091. PAS. 1 hit.
SM00448. REC. 1 hit.
[Graphical view ]
SUPFAMi SSF47226. SSF47226. 1 hit.
SSF47384. SSF47384. 1 hit.
SSF52172. SSF52172. 1 hit.
SSF55785. SSF55785. 1 hit.
SSF55874. SSF55874. 1 hit.
TIGRFAMsi TIGR00229. sensory_box. 1 hit.
PROSITEi PS50109. HIS_KIN. 1 hit.
PS50894. HPT. 1 hit.
PS50113. PAC. 1 hit.
PS50112. PAS. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publicationsDownload
  1. "The arcB gene of Escherichia coli encodes a sensor-regulator protein for anaerobic repression of the arc modulon."
    Iuchi S., Matsuda Z., Fujiwara T., Lin E.C.C.
    Mol. Microbiol. 4:715-727(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "The complete genome sequence of Escherichia coli K-12."
    Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
    Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Escherichia coli K-12: a cooperatively developed annotation snapshot -- 2005."
    Riley M., Abe T., Arnaud M.B., Berlyn M.K.B., Blattner F.R., Chaudhuri R.R., Glasner J.D., Horiuchi T., Keseler I.M., Kosuge T., Mori H., Perna N.T., Plunkett G. III, Rudd K.E., Serres M.H., Thomas G.H., Thomson N.R., Wishart D., Wanner B.L.
    Nucleic Acids Res. 34:1-9(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION TO 469-470.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "In vitro phosphorylation study of the arc two-component signal transduction system of Escherichia coli."
    Georgellis D., Lynch A.S., Lin E.C.C.
    J. Bacteriol. 179:5429-5435(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
    Strain: M15.
  6. "Signal decay through a reverse phosphorelay in the arc two-component signal transduction system."
    Georgellis D., Kwon O., De Wulf P., Lin E.C.C.
    J. Biol. Chem. 273:32864-32869(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
    Strain: M15.
  7. "Phosphorelay as the sole physiological route of signal transmission by the arc two-component system of Escherichia coli."
    Kwon O., Georgellis D., Lin E.C.C.
    J. Bacteriol. 182:3858-3862(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF HIS-292; ASP-576 AND HIS-717.
    Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
  8. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
    Strain: K12 / MG1655 / ATCC 47076.
  9. "Insights into multistep phosphorelay from the crystal structure of the C-terminal HPt domain of ArcB."
    Kato M., Mizuno T., Shimizu T., Hakoshima T.
    Cell 88:717-723(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.06 ANGSTROMS) OF 660-778.
  10. "Crystallization of a complex between a novel C-terminal transmitter, HPt domain, of the anaerobic sensor kinase ArcB and the chemotaxis response regulator CheY."
    Kato M., Mizuno T., Hakoshima T.
    Acta Crystallogr. D 54:140-142(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 659-776 IN COMPLEX WITH CHEY.
  11. "Refined structure of the histidine-containing-phosphotransfer (HPt) domain of the anaerobic sensor kinase ArcB from Escherichia coli at 1.57-A resolution."
    Kato M., Mizuno T., Shimizu T., Hakoshima T.
    Acta Crystallogr. D 55:1842-1849(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.57 ANGSTROMS) OF 659-776.

Entry nameiARCB_ECOLI
AccessioniPrimary (citable) accession number: P0AEC3
Secondary accession number(s): P22763, Q2M902
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: March 19, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene

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