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P0AEF0

- DNAC_ECOLI

UniProt

P0AEF0 - DNAC_ECOLI

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Protein
DNA replication protein DnaC
Gene
dnaC, dnaD, b4361, JW4325
Organism
Escherichia coli (strain K12)
Status
Reviewed - - Experimental evidence at protein leveli

Functioni

This protein is required for chromosomal replication. It forms, in concert with DnaB protein and other prepriming proteins DnaT, N, N', N'' a prepriming protein complex on the specific site of the template DNA recognized by protein N'.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier
Sitei69 – 691Probably involved in the interaction with the DnaB protein

GO - Molecular functioni

  1. ATP bindingInferred from electronic annotationi Source: UniProtKB-KW
  2. nucleoside-triphosphatase activityInferred from electronic annotationi Source: InterPro
  3. protein bindingInferred from physical interactioni PubMed 15690043PubMed 20129058PubMed 23562643PubMed 24561554PubMed 8663105 Source: IntAct
Complete GO annotation...

GO - Biological processi

  1. DNA replication, synthesis of RNA primerInferred from electronic annotationi Source: UniProtKB-KW
  2. DNA strand elongation involved in DNA replicationInferred from genetic interactioni PubMed 6277866 Source: EcoCyc
  1. nucleoside-triphosphatase activityInferred from electronic annotationi Source: InterPro
Complete GO annotation...

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10237-MONOMER.
ECOL316407:JW4325-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication protein DnaC
Gene namesi
Name:dnaC
Synonyms:dnaD
Ordered Locus Names:b4361, JW4325
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10237. dnaC.

Subcellular locationi

GO - Cellular componenti

  1. primosome complexInferred from electronic annotationi Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Primosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier
Chaini1 – 245245DNA replication protein DnaC
PRO_0000079954

Proteomic databases

PaxDbiP0AEF0.
PRIDEiP0AEF0.

Expressioni

Gene expression databases

GenevestigatoriP0AEF0.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
dnaBP0ACB09EBI-549012,EBI-548978

Protein-protein interaction databases

DIPiDIP-9457N.
IntActiP0AEF0. 13 interactions.
MINTiMINT-1276712.
STRINGi511145.b4361.

Structurei

3D structure databases

ProteinModelPortaliP0AEF0.
SMRiP0AEF0. Positions 53-232.

Family & Domainsi

Sequence similaritiesi

Belongs to the DnaC family.

Phylogenomic databases

eggNOGiCOG1484.
HOGENOMiHOG000120230.
KOiK02315.
OMAiVIFNWES.
OrthoDBiEOG6TR0CP.
PhylomeDBiP0AEF0.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR028350. DNAC/ATP-binding.
IPR002611. IstB_ATP-bd.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01695. IstB_IS21. 1 hit.
[Graphical view]
PIRSFiPIRSF003073. DNAC_TnpB_IstB. 1 hit.
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AEF0-1 [UniParc]FASTA

« Hide

MKNVGDLMQR LQKMMPAHIK PAFKTGEELL AWQKEQGAIR SAALERENRA    50
MKMQRTFNRS GIRPLHQNCS FENYRVECEG QMNALSKARQ YVEEFDGNIA 100
SFIFSGKPGT GKNHLAAAIC NELLLRGKSV LIITVADIMS AMKDTFRNSG 150
TSEEQLLNDL SNVDLLVIDE IGVQTESKYE KVIINQIVDR RSSSKRPTGM 200
LTNSNMEEMT KLLGERVMDR MRLGNSLWVI FNWDSYRSRV TGKEY 245
Length:245
Mass (Da):27,935
Last modified:December 6, 2005 - v1
Checksum:iC5CC7232E21F11F1
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier
Sequence conflicti6 – 61D → A1 Publication
Sequence conflicti6 – 61D → A1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J04030 Genomic DNA. Translation: AAA23700.1.
U14003 Genomic DNA. Translation: AAA97260.1.
U00096 Genomic DNA. Translation: AAC77317.1.
AP009048 Genomic DNA. Translation: BAE78351.1.
PIRiXMECNC. A28484.
RefSeqiNP_418781.1. NC_000913.3.
YP_492492.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC77317; AAC77317; b4361.
BAE78351; BAE78351; BAE78351.
GeneIDi12933761.
948864.
KEGGiecj:Y75_p4246.
eco:b4361.
PATRICi32124330. VBIEscCol129921_4508.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J04030 Genomic DNA. Translation: AAA23700.1 .
U14003 Genomic DNA. Translation: AAA97260.1 .
U00096 Genomic DNA. Translation: AAC77317.1 .
AP009048 Genomic DNA. Translation: BAE78351.1 .
PIRi XMECNC. A28484.
RefSeqi NP_418781.1. NC_000913.3.
YP_492492.1. NC_007779.1.

3D structure databases

ProteinModelPortali P0AEF0.
SMRi P0AEF0. Positions 53-232.
ModBasei Search...

Protein-protein interaction databases

DIPi DIP-9457N.
IntActi P0AEF0. 13 interactions.
MINTi MINT-1276712.
STRINGi 511145.b4361.

Proteomic databases

PaxDbi P0AEF0.
PRIDEi P0AEF0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC77317 ; AAC77317 ; b4361 .
BAE78351 ; BAE78351 ; BAE78351 .
GeneIDi 12933761.
948864.
KEGGi ecj:Y75_p4246.
eco:b4361.
PATRICi 32124330. VBIEscCol129921_4508.

Organism-specific databases

EchoBASEi EB0233.
EcoGenei EG10237. dnaC.

Phylogenomic databases

eggNOGi COG1484.
HOGENOMi HOG000120230.
KOi K02315.
OMAi VIFNWES.
OrthoDBi EOG6TR0CP.
PhylomeDBi P0AEF0.

Enzyme and pathway databases

BioCyci EcoCyc:EG10237-MONOMER.
ECOL316407:JW4325-MONOMER.

Miscellaneous databases

PROi P0AEF0.

Gene expression databases

Genevestigatori P0AEF0.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR003593. AAA+_ATPase.
IPR028350. DNAC/ATP-binding.
IPR002611. IstB_ATP-bd.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF01695. IstB_IS21. 1 hit.
[Graphical view ]
PIRSFi PIRSF003073. DNAC_TnpB_IstB. 1 hit.
SMARTi SM00382. AAA. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publicationsDownload
  1. "Structure of Escherichia coli dnaC. Identification of a cysteine residue possibly involved in association with dnaB protein."
    Nakayama N., Bond M.W., Miyajima A., Kobori J., Arai K.
    J. Biol. Chem. 262:10475-10480(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Operon structure of dnaT and dnaC genes essential for normal and stable DNA replication of Escherichia coli chromosome."
    Masai H., Arai K.
    J. Biol. Chem. 263:15083-15093(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  3. "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
    Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
    Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "The complete genome sequence of Escherichia coli K-12."
    Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
    Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Cloning of the Escherichia coli gene for primosomal protein I: the relationship to dnaT, essential for chromosomal DNA replication."
    Masai H., Bond M.W., Arai K.
    Proc. Natl. Acad. Sci. U.S.A. 83:1256-1260(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-14.
    Strain: K12.
  7. "Escherichia coli proteome analysis using the gene-protein database."
    VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
    Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY 2D-GEL.

Entry nameiDNAC_ECOLI
AccessioniPrimary (citable) accession number: P0AEF0
Secondary accession number(s): P07905, Q2M5V5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: December 6, 2005
Last modified: June 11, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene

External Data

Dasty 3

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