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P0AFJ5

- PHOB_ECOLI

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Protein
Phosphate regulon transcriptional regulatory protein PhoB
Gene
phoB, b0399, JW0389

Gene:

phoB, b0399, JW0389
Organism
Escherichia coli (strain K12)
Status
Reviewed - - Experimental evidence at protein leveli

UniProt

P0AFJ5 - PHOB_ECOLI

Protein:

Phosphate regulon transcriptional regulatory protein PhoB
Protein Existence: Experimental evidence at protein level

Functioni

This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by PhoB and PhoR when phosphate is limited.

GO - Molecular functioni

  1. DNA bindingInferred from electronic annotationi Source: UniProtKB-KW
  2. bacterial-type RNA polymerase holo enzyme bindingInferred from mutant phenotypei PubMed 8648643 Source: EcoCyc
  3. phosphorelay response regulator activityInferred from electronic annotationi Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. DNA bindingInferred from electronic annotationi Source: UniProtKB-KW
  2. bacterial-type RNA polymerase holo enzyme bindingInferred from mutant phenotypei PubMed 8648643 Source: EcoCyc
  3. phosphorelay response regulator activityInferred from electronic annotationi Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Phosphate transport, Transcription, Transcription regulation, Transport, Two-component regulatory system

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:PHOB-MONOMER.
ECOL316407:JW0389-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphate regulon transcriptional regulatory protein PhoB
Gene namesi
Name:phoB
Ordered Locus Names:b0399, JW0389
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10728. phoB.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasmInferred from electronic annotationi Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier
Chaini1 – 229229Phosphate regulon transcriptional regulatory protein PhoB
PRO_0000081186

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier
Modified residuei5314-aspartylphosphate

Post-translational modificationi

Phosphorylated by PhoR or CreC.

Keywords - PTMi

Phosphoprotein

Expressioni

Gene expression databases

GenevestigatoriP0AFJ5.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-1116564,EBI-1116564

Protein-protein interaction databases

DIPiDIP-35852N.
IntActiP0AFJ5. 20 interactions.
MINTiMINT-1313174.
STRINGi511145.b0399.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier
Beta strandi4 – 85
Helixi12 – 2413
Beta strandi28 – 325
Helixi35 – 395
Beta strandi43 – 453
Beta strandi48 – 569
Helixi61 – 699
Turni72 – 765
Beta strandi79 – 846
Helixi87 – 926
Beta strandi100 – 1067
Helixi109 – 12113
Beta strandi132 – 1343
Beta strandi137 – 1404
Turni141 – 1444
Beta strandi145 – 1484
Beta strandi151 – 1533
Helixi157 – 16812
Beta strandi171 – 1744
Helixi176 – 1838
Beta strandi186 – 1883
Helixi193 – 20614
Helixi207 – 2093
Helixi211 – 2144
Beta strandi215 – 2184
Turni219 – 2213
Beta strandi222 – 2254

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1B00X-ray1.88A/B1-127[»]
1GXPX-ray2.50A/B/E/F124-229[»]
1GXQX-ray2.00A124-229[»]
1QQINMR-A126-229[»]
1ZESX-ray1.90A/B/C1-124[»]
2IYNX-ray2.08A/B/C1-127[»]
2JB9X-ray1.70A/B1-127[»]
2JBAX-ray1.45A/B1-127[»]
2Z33NMR-A126-229[»]
3T72X-ray4.331/4/5/8/9/A/B/E/F/I/J/M/N/R/S/V/W/Z/c/d/g/h/k/l128-229[»]
ProteinModelPortaliP0AFJ5.
SMRiP0AFJ5. Positions 2-123, 125-229.

Miscellaneous databases

EvolutionaryTraceiP0AFJ5.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier
Domaini1 – 120120Response regulatory

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0745.
HOGENOMiHOG000034819.
KOiK07657.
OMAiPWQVFTR.
OrthoDBiEOG6G4VQG.
ProtClustDBiPRK10161.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR001867. Sig_transdc_resp-reg_C.
IPR011879. Sig_transdc_resp-reg_PhoB.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00072. Response_reg. 1 hit.
PF00486. Trans_reg_C. 1 hit.
[Graphical view]
SMARTiSM00448. REC. 1 hit.
SM00862. Trans_reg_C. 1 hit.
[Graphical view]
SUPFAMiSSF52172. SSF52172. 1 hit.
TIGRFAMsiTIGR02154. PhoB. 1 hit.
PROSITEiPS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AFJ5-1 [UniParc]FASTA

« Hide

MARRILVVED EAPIREMVCF VLEQNGFQPV EAEDYDSAVN QLNEPWPDLI    50
LLDWMLPGGS GIQFIKHLKR ESMTRDIPVV MLTARGEEED RVRGLETGAD 100
DYITKPFSPK ELVARIKAVM RRISPMAVEE VIEMQGLSLD PTSHRVMAGE 150
EPLEMGPTEF KLLHFFMTHP ERVYSREQLL NHVWGTNVYV EDRTVDVHIR 200
RLRKALEPGG HDRMVQTVRG TGYRFSTRF 229
Length:229
Mass (Da):26,433
Last modified:August 1, 1988 - v1
Checksum:iEB46BA282F1F2C23
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X04026 Genomic DNA. Translation: CAA27659.1.
U73857 Genomic DNA. Translation: AAB18123.1.
U00096 Genomic DNA. Translation: AAC73502.1.
AP009048 Genomic DNA. Translation: BAE76179.1.
PIRiRGECFB. A24256.
RefSeqiNP_414933.1. NC_000913.3.
YP_488691.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC73502; AAC73502; b0399.
BAE76179; BAE76179; BAE76179.
GeneIDi12930822.
945046.
KEGGiecj:Y75_p0387.
eco:b0399.
PATRICi32115943. VBIEscCol129921_0413.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X04026 Genomic DNA. Translation: CAA27659.1 .
U73857 Genomic DNA. Translation: AAB18123.1 .
U00096 Genomic DNA. Translation: AAC73502.1 .
AP009048 Genomic DNA. Translation: BAE76179.1 .
PIRi RGECFB. A24256.
RefSeqi NP_414933.1. NC_000913.3.
YP_488691.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1B00 X-ray 1.88 A/B 1-127 [» ]
1GXP X-ray 2.50 A/B/E/F 124-229 [» ]
1GXQ X-ray 2.00 A 124-229 [» ]
1QQI NMR - A 126-229 [» ]
1ZES X-ray 1.90 A/B/C 1-124 [» ]
2IYN X-ray 2.08 A/B/C 1-127 [» ]
2JB9 X-ray 1.70 A/B 1-127 [» ]
2JBA X-ray 1.45 A/B 1-127 [» ]
2Z33 NMR - A 126-229 [» ]
3T72 X-ray 4.33 1/4/5/8/9/A/B/E/F/I/J/M/N/R/S/V/W/Z/c/d/g/h/k/l 128-229 [» ]
ProteinModelPortali P0AFJ5.
SMRi P0AFJ5. Positions 2-123, 125-229.
ModBasei Search...

Protein-protein interaction databases

DIPi DIP-35852N.
IntActi P0AFJ5. 20 interactions.
MINTi MINT-1313174.
STRINGi 511145.b0399.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC73502 ; AAC73502 ; b0399 .
BAE76179 ; BAE76179 ; BAE76179 .
GeneIDi 12930822.
945046.
KEGGi ecj:Y75_p0387.
eco:b0399.
PATRICi 32115943. VBIEscCol129921_0413.

Organism-specific databases

EchoBASEi EB0721.
EcoGenei EG10728. phoB.

Phylogenomic databases

eggNOGi COG0745.
HOGENOMi HOG000034819.
KOi K07657.
OMAi PWQVFTR.
OrthoDBi EOG6G4VQG.
ProtClustDBi PRK10161.

Enzyme and pathway databases

BioCyci EcoCyc:PHOB-MONOMER.
ECOL316407:JW0389-MONOMER.

Miscellaneous databases

EvolutionaryTracei P0AFJ5.
PROi P0AFJ5.

Gene expression databases

Genevestigatori P0AFJ5.

Family and domain databases

Gene3Di 1.10.10.10. 1 hit.
InterProi IPR011006. CheY-like_superfamily.
IPR001867. Sig_transdc_resp-reg_C.
IPR011879. Sig_transdc_resp-reg_PhoB.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view ]
Pfami PF00072. Response_reg. 1 hit.
PF00486. Trans_reg_C. 1 hit.
[Graphical view ]
SMARTi SM00448. REC. 1 hit.
SM00862. Trans_reg_C. 1 hit.
[Graphical view ]
SUPFAMi SSF52172. SSF52172. 1 hit.
TIGRFAMsi TIGR02154. PhoB. 1 hit.
PROSITEi PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publicationsDownload
  1. "Nucleotide sequence of the phoB gene, the positive regulatory gene for the phosphate regulon of Escherichia coli K-12."
    Makino K., Shinagawa H., Amemura M., Nakata A.
    J. Mol. Biol. 190:37-44(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Sequence of minutes 4-25 of Escherichia coli."
    Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "The complete genome sequence of Escherichia coli K-12."
    Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
    Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Three-dimensional crystal structure of the transcription factor PhoB receiver domain."
    Sola M., Gomis-Rueth F.-X., Serrano L., Gonzalez A., Coll M.
    J. Mol. Biol. 285:675-687(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 1-126.
  6. "Structural comparison of the PhoB and OmpR DNA-binding/transactivation domains and the arrangement of PhoB molecules on the phosphate box."
    Okamura H., Hanaoka S., Nagadoi A., Makino K., Nishimura Y.
    J. Mol. Biol. 295:1225-1236(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 126-229.

Entry nameiPHOB_ECOLI
AccessioniPrimary (citable) accession number: P0AFJ5
Secondary accession number(s): P08402, Q2MC27
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: March 19, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene

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