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P0AG86

- SECB_ECOLI

UniProt

P0AG86 - SECB_ECOLI

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Protein
Protein-export protein SecB
Gene
secB, b3609, JW3584
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

One of the proteins required for the normal export of some preproteins out of the cell cytoplasm. It is a molecular chaperone that binds to a subset of precursor proteins, maintaining them in a translocation-competent state. It also specifically binds to its receptor SecA. Its substrates include AmpC, DegP, FhuA, FkpA, GBP, LamB, MalE (MBP), OmpA, OmpF, OmpT, OmpX, OppA, PhoE, TolB, TolC, YbgF, YcgK, YgiW and YncE.UniRule annotation

GO - Molecular functioni

  1. protein bindingInferred from physical interactioni PubMed 15690043PubMed 21037004 Source: IntAct
  2. unfolded protein bindingInferred from direct assayi PubMed 6403503 Source: EcoCyc
Complete GO annotation...

GO - Biological processi

  1. protein foldingInferred from electronic annotationi Source: UniProtKB-HAMAP
  2. protein targetingInferred from mutant phenotypei PubMed 6403503 Source: EcoliWiki
  3. protein tetramerizationInferred from direct assayi PubMed 2649892 Source: EcoCyc
  4. protein transportInferred from mutant phenotypei PubMed 6403503 Source: EcoliWiki
  5. protein transport by the Sec complexInferred from mutant phenotypei PubMed 6403503 Source: EcoliWiki
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Protein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciEcoCyc:SECB.
ECOL316407:JW3584-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-export protein SecB
Gene namesi
Name:secB
Ordered Locus Names:b3609, JW3584
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10937. secB.

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytosolInferred from direct assayi PubMed 2649892 Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Growth continues, but export of its substrates is blocked. Expression of chaperones DnaK, GroEL/ES, ClpB, and HslU increases.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 155155Protein-export protein SecBUniRule annotation
PRO_0000055370Add
BLAST

Proteomic databases

PaxDbiP0AG86.
PRIDEiP0AG86.

PTM databases

PhosSiteiP0810436.

Expressioni

Gene expression databases

GenevestigatoriP0AG86.

Interactioni

Subunit structurei

Homotetramer, a dimer of dimers. One homotetramer interacts with 1 SecA dimer.

Binary interactionsi

WithEntry#Exp.IntActNotes
secAP104084EBI-555877,EBI-543213

Protein-protein interaction databases

DIPiDIP-47924N.
IntActiP0AG86. 14 interactions.
MINTiMINT-1248893.
STRINGi511145.b3609.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi10 – 2415
Helixi28 – 314
Beta strandi39 – 5214
Beta strandi55 – 6814
Beta strandi71 – 8818
Helixi91 – 999
Helixi101 – 12121
Helixi133 – 1419

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1QYNX-ray2.35A/B/C/D1-153[»]
ProteinModelPortaliP0AG86.
SMRiP0AG86. Positions 9-144.

Miscellaneous databases

EvolutionaryTraceiP0AG86.

Family & Domainsi

Sequence similaritiesi

Belongs to the SecB family.

Phylogenomic databases

eggNOGiCOG1952.
HOGENOMiHOG000218192.
KOiK03071.
OMAiGSFPQLN.
OrthoDBiEOG6DVJZZ.
PhylomeDBiP0AG86.

Family and domain databases

Gene3Di3.10.420.10. 1 hit.
HAMAPiMF_00821. SecB.
InterProiIPR003708. SecB.
[Graphical view]
PfamiPF02556. SecB. 1 hit.
[Graphical view]
PRINTSiPR01594. SECBCHAPRONE.
SUPFAMiSSF54611. SSF54611. 1 hit.
TIGRFAMsiTIGR00809. secB. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AG86-1 [UniParc]FASTA

« Hide

MSEQNNTEMT FQIQRIYTKD ISFEAPNAPH VFQKDWQPEV KLDLDTASSQ    50
LADDVYEVVL RVTVTASLGE ETAFLCEVQQ GGIFSIAGIE GTQMAHCLGA 100
YCPNILFPYA RECITSMVSR GTFPQLNLAP VNFDALFMNY LQQQAGEGTE 150
EHQDA 155
Length:155
Mass (Da):17,277
Last modified:December 20, 2005 - v1
Checksum:i00514C5F393A643D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M24489 Genomic DNA. Translation: AAA83907.1.
U00039 Genomic DNA. Translation: AAB18586.1.
U00096 Genomic DNA. Translation: AAC76633.1.
AP009048 Genomic DNA. Translation: BAE77683.1.
PIRiVXECS. JS0126.
RefSeqiNP_418066.1. NC_000913.3.
YP_491824.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC76633; AAC76633; b3609.
BAE77683; BAE77683; BAE77683.
GeneIDi12932409.
948123.
KEGGiecj:Y75_p3565.
eco:b3609.
PATRICi32122703. VBIEscCol129921_3728.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M24489 Genomic DNA. Translation: AAA83907.1 .
U00039 Genomic DNA. Translation: AAB18586.1 .
U00096 Genomic DNA. Translation: AAC76633.1 .
AP009048 Genomic DNA. Translation: BAE77683.1 .
PIRi VXECS. JS0126.
RefSeqi NP_418066.1. NC_000913.3.
YP_491824.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1QYN X-ray 2.35 A/B/C/D 1-153 [» ]
ProteinModelPortali P0AG86.
SMRi P0AG86. Positions 9-144.
ModBasei Search...

Protein-protein interaction databases

DIPi DIP-47924N.
IntActi P0AG86. 14 interactions.
MINTi MINT-1248893.
STRINGi 511145.b3609.

PTM databases

PhosSitei P0810436.

Proteomic databases

PaxDbi P0AG86.
PRIDEi P0AG86.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC76633 ; AAC76633 ; b3609 .
BAE77683 ; BAE77683 ; BAE77683 .
GeneIDi 12932409.
948123.
KEGGi ecj:Y75_p3565.
eco:b3609.
PATRICi 32122703. VBIEscCol129921_3728.

Organism-specific databases

EchoBASEi EB0930.
EcoGenei EG10937. secB.

Phylogenomic databases

eggNOGi COG1952.
HOGENOMi HOG000218192.
KOi K03071.
OMAi GSFPQLN.
OrthoDBi EOG6DVJZZ.
PhylomeDBi P0AG86.

Enzyme and pathway databases

BioCyci EcoCyc:SECB.
ECOL316407:JW3584-MONOMER.

Miscellaneous databases

EvolutionaryTracei P0AG86.
PROi P0AG86.

Gene expression databases

Genevestigatori P0AG86.

Family and domain databases

Gene3Di 3.10.420.10. 1 hit.
HAMAPi MF_00821. SecB.
InterProi IPR003708. SecB.
[Graphical view ]
Pfami PF02556. SecB. 1 hit.
[Graphical view ]
PRINTSi PR01594. SECBCHAPRONE.
SUPFAMi SSF54611. SSF54611. 1 hit.
TIGRFAMsi TIGR00809. secB. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publicationsDownload
  1. "Characterization of the Escherichia coli protein-export gene secB."
    Kumamoto C.A., Nault A.K.
    Gene 75:167-175(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes."
    Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.
    Nucleic Acids Res. 22:2576-2586(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "The complete genome sequence of Escherichia coli K-12."
    Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
    Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Escherichia coli proteome analysis using the gene-protein database."
    VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
    Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY 2D-GEL.
  6. "Crystal structure of SecB from Escherichia coli."
    Dekker C., de Kruijff B., Gros P.
    J. Struct. Biol. 144:313-319(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.35 ANGSTROMS).
  7. "Defining the role of the Escherichia coli chaperone SecB using comparative proteomics."
    Baars L., Ytterberg A.J., Drew D., Wagner S., Thilo C., van Wijk K.J., de Gier J.W.
    J. Biol. Chem. 281:10024-10034(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBSTRATES, DISRUPTION PHENOTYPE.
    Strain: K12 / MC4100.
  8. "Bacterial protein secretion through the translocase nanomachine."
    Papanikou E., Karamanou S., Economou A.
    Nat. Rev. Microbiol. 5:839-851(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW OF PROTEIN SECRETION.

Entry nameiSECB_ECOLI
AccessioniPrimary (citable) accession number: P0AG86
Secondary accession number(s): P15040, Q2M7S3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: June 11, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene

External Data

Dasty 3

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