Reviewed, UniProtKB/Swiss-Prot P0C605 (KGP1A_MOUSE)

Names and origin

Protein names

Recommended name:
    cGMP-dependent protein kinase 1, alpha isozyme
      Short name(s)=CGK 1 alpha, cGKI-alpha
    EC=2.7.11.12

Gene names

Name:	Prkg1
Synonyms:	Prkgr1a

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	671 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation	Binding of cGMP results in enzyme activation By similarity.
Subunit structure	Antiparallel homodimer By similarity.
Post-translational modification	65 kDa monomer is produced by proteolytic cleavage By similarity.
Miscellaneous	Exhibit a substrate specificity similar but not identical to that of CAK By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cGMP subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 2 cyclic nucleotide-binding domains. Contains 1 protein kinase domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Domain	Coiled coil
Ligand	ATP-binding Nucleotide-binding cGMP cGMP-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Acetylation Phosphoprotein
Gene Ontology (GO)
None. [Check GOA]

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform CGK1-alpha (identifier: P0C605-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform CGK1-beta (identifier: Q9Z0Z0-1)

The sequence of this isoform can be found in the external entry Q9Z0Z0-1.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 671	670	cGMP-dependent protein kinase 1, alpha isozyme
Regions
	Domain	360 – 619	260	Protein kinase
	Domain	620 – 671	52	AGC-kinase C-terminal
	Nucleotide binding	103 – 218	116	cNMP 1
	Nucleotide binding	221 – 342	122	cNMP 2
	Nucleotide binding	366 – 374	9	ATP By similarity
	Region	2 – 102	101	Dimerization By similarity
	Coiled coil	6 – 48	43	Potential
Sites
	Active site	484	1	Proton acceptor By similarity
	Binding site	390	1	ATP By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylserine By similarity
	Modified residue	59	1	Phosphothreonine; by autocatalysis By similarity
	Disulfide bond	43		Interchain By similarity

Sequences

	Sequence		Length	Mass (Da)
	Isoform CGK1-alpha [UniParc]. Last modified January 15, 2008. Version 1. Checksum: 51878E2D27F26A22	FASTA	671	76,350
	10 20 30 40 50 60 MSELEEDFAK ILMLKEERIK ELEKRLSEKE EEIQELKRKL HKCQSVLPVP STHIGPRTTR 70 80 90 100 110 120 AQGISAEPQT YRSFHDLRQA FRKFTKSERS KDLIKEAILD NDFMKNLELS QIQEIVDCMY 130 140 150 160 170 180 PVEYGKDSCI IKEGDVGSLV YVMEDGKVEV TKEGVKLCTM GPGKVFGELA ILYNCTRTAT 190 200 210 220 230 240 VKTLVNVKLW AIDRQCFQTI MMRTGLIKHT EYMEFLKSVP TFQSLPDEIL SKLADVLEET 250 260 270 280 290 300 HYENGEYIIR QGARGDTFFI ISKGQVNVTR EDSPSEDPVF LRTLGKGDWF GEKALQGEDV 310 320 330 340 350 360 RTANVIAAEA VTCLVIDRDS FKHLIGGLDD VSNKAYEDAE AKAKYEAEAA FFANLKLSDF 370 380 390 400 410 420 NIIDTLGVGG FGRVELVQLK SEESKTFAMK ILKKRHIVDT RQQEHIRSEK QIMQGAHSDF 430 440 450 460 470 480 IVRLYRTFKD SKYLYMLMEA CLGGELWTIL RDRGSFEDST TRFYTACVVE AFAYLHSKGI 490 500 510 520 530 540 IYRDLKPENL ILDHRGYAKL VDFGFAKKIG FGKKTWTFCG TPEYVAPEII LNKGHDISAD 550 560 570 580 590 600 YWSLGILMYE LLTGSPPFSG PDPMKTYNII LRGIDMIEFP KKIAKNAANL IKKLCRDNPS 610 620 630 640 650 660 ERLGNLKNGV KDIQKHKWFE GFNWEGLRKG TLTPPIIPSV ASPTDTSNFD SFPEDSDEPP 670 PDDNSGWDID F
	Isoform CGK1-beta. See Q9Z0Z0.		–	–

References

[1]	The mouse genome sequencing consortium Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC102751 Genomic DNA. No translation available. AC103405 Genomic DNA. No translation available. AC108399 Genomic DNA. No translation available. AC116507 Genomic DNA. No translation available. AC119158 Genomic DNA. No translation available. AC122548 Genomic DNA. No translation available.
RefSeq	NP_001013855.1.
UniGene	Mm.381170 Mm.381172
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	19091.
KEGG	mmu:19091.
Gene expression databases
CleanEx	MM_PRKG1.
Family and domain databases
InterPro	IPR002373. cAMP/cGMP_kin. IPR016232. cGMP-dependent_protein_kinase. IPR002374. cGMP_kinase_core. IPR000595. cNMP_bd. IPR000961. Pkinase_C. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_bd_CS. IPR014710. RmlC-like_jellyroll. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Gene3D	G3DSA:2.60.120.10. RmlC-like_jellyroll. 2 hits.
Pfam	PF00027. cNMP_binding. 2 hits. PF00069. Pkinase. 1 hit. [Graphical view]
PIRSF	PIRSF000559. cGMP-dep_kinase. 1 hit.
PRINTS	PR00103. CAMPKINASE. PR00104. CGMPKINASE.
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00100. cNMP. 2 hits. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS00888. CNMP_BINDING_1. 2 hits. PS00889. CNMP_BINDING_2. 2 hits. PS50042. CNMP_BINDING_3. 2 hits. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

KGP1A_MOUSE

Accession

Primary (citable) accession number: P0C605

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 15, 2008
Last modified:	July 22, 2008
This is version 8 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)