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P10728

- SP2AB_BACSU

UniProt

P10728 - SP2AB_BACSU

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Protein
Anti-sigma F factor
Gene
spoIIAB, BSU23460
Organism
Bacillus subtilis (strain 168)
Status
Reviewed - - Experimental evidence at protein leveli

Functioni

Binds to sigma F and blocks its ability to form an RNA polymerase holoenzyme (E-sigma F). Phosphorylates SpoIIAA on a serine residue. This phosphorylation may enable SpoIIAA to act as an anti-anti-sigma factor that counteracts SpoIIAB and thus releases sigma F from inhibition.2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.UniRule annotation

GO - Molecular functioni

  1. ATP bindingInferred from electronic annotationi Source: UniProtKB-KW
  2. protein serine/threonine kinase activityInferred from electronic annotationi Source: UniProtKB-KW
  3. sigma factor antagonist activityInferred from electronic annotationi Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. negative regulation of transcription, DNA-templatedInferred from electronic annotationi Source: InterPro
  2. sporulation resulting in formation of a cellular sporeInferred from electronic annotationi Source: UniProtKB-KW
  1. protein serine/threonine kinase activityInferred from electronic annotationi Source: UniProtKB-KW
  2. sigma factor antagonist activityInferred from electronic annotationi Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Sporulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU23460-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Anti-sigma F factor (EC:2.7.11.1)
Alternative name(s):
Stage II sporulation protein AB
Gene namesi
Name:spoIIAB
Ordered Locus Names:BSU23460
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570: Chromosome

Organism-specific databases

GenoListiBSU23460. [Micado]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier
Chaini1 – 146146Anti-sigma F factorUniRule annotation
PRO_0000203561

Proteomic databases

PaxDbiP10728.

Expressioni

Developmental stagei

Interaction with SpoIIAB inhibits sigma F activity throughout the cell before the formation of the asymmetric septum; after septation the interaction is confined to the mother cell, and sigma F activity is released in the prespore.UniRule annotation

Interactioni

Protein-protein interaction databases

STRINGi224308.BSU23460.

Structurei

3D structure databases

ProteinModelPortaliP10728.
SMRiP10728. Positions 2-137.

Family & Domainsi

Sequence similaritiesi

Belongs to the anti-sigma-factor family.

Phylogenomic databases

eggNOGiCOG2172.
HOGENOMiHOG000269931.
KOiK06379.
OMAiDSSPEMG.
OrthoDBiEOG6S7XW5.
PhylomeDBiP10728.

Family and domain databases

Gene3Di3.30.565.10. 1 hit.
HAMAPiMF_00637. Anti_sigma_F.
InterProiIPR010194. Anti-sigma_F.
IPR003594. HATPase_ATP-bd.
[Graphical view]
PfamiPF02518. HATPase_c. 1 hit.
[Graphical view]
SMARTiSM00387. HATPase_c. 1 hit.
[Graphical view]
SUPFAMiSSF55874. SSF55874. 1 hit.
TIGRFAMsiTIGR01925. spIIAB. 1 hit.

Sequencei

Sequence statusi: Complete.

P10728-1 [UniParc]FASTA

« Hide

MKNEMHLEFS ALSQNESFAR VTVASFIAQL DPTMDELTEI KTVVSEAVTN    50
AIIHGYEENC EGKVYISVTL EDHVVYMTIR DEGLGITDLE EARQPLFTTK 100
PELERSGMGF TIMENFMDDV SIDSSPEMGT TIRLTKHLSK SKALCN 146
Length:146
Mass (Da):16,356
Last modified:February 1, 1991 - v1
Checksum:i141A28D41EDEC997
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M17643 Genomic DNA. Translation: AAA22790.1.
D84432 Genomic DNA. Translation: BAA12654.1.
AL009126 Genomic DNA. Translation: CAB14278.1.
PIRiB55646.
RefSeqiNP_390227.1. NC_000964.3.

Genome annotation databases

EnsemblBacteriaiCAB14278; CAB14278; BSU23460.
GeneIDi938930.
KEGGibsu:BSU23460.
PATRICi18976511. VBIBacSub10457_2446.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M17643 Genomic DNA. Translation: AAA22790.1 .
D84432 Genomic DNA. Translation: BAA12654.1 .
AL009126 Genomic DNA. Translation: CAB14278.1 .
PIRi B55646.
RefSeqi NP_390227.1. NC_000964.3.

3D structure databases

ProteinModelPortali P10728.
SMRi P10728. Positions 2-137.
ModBasei Search...

Protein-protein interaction databases

STRINGi 224308.BSU23460.

Proteomic databases

PaxDbi P10728.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB14278 ; CAB14278 ; BSU23460 .
GeneIDi 938930.
KEGGi bsu:BSU23460.
PATRICi 18976511. VBIBacSub10457_2446.

Organism-specific databases

GenoListi BSU23460. [Micado ]

Phylogenomic databases

eggNOGi COG2172.
HOGENOMi HOG000269931.
KOi K06379.
OMAi DSSPEMG.
OrthoDBi EOG6S7XW5.
PhylomeDBi P10728.

Enzyme and pathway databases

BioCyci BSUB:BSU23460-MONOMER.

Family and domain databases

Gene3Di 3.30.565.10. 1 hit.
HAMAPi MF_00637. Anti_sigma_F.
InterProi IPR010194. Anti-sigma_F.
IPR003594. HATPase_ATP-bd.
[Graphical view ]
Pfami PF02518. HATPase_c. 1 hit.
[Graphical view ]
SMARTi SM00387. HATPase_c. 1 hit.
[Graphical view ]
SUPFAMi SSF55874. SSF55874. 1 hit.
TIGRFAMsi TIGR01925. spIIAB. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publicationsDownload
  1. "Nucleotide sequence of sporulation locus spoIIA in Bacillus subtilis."
    Fort P., Piggot P.J.
    J. Gen. Microbiol. 130:2147-2153(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the Bacillus subtilis genome containing the skin element and many sporulation genes."
    Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M., Kobayashi Y.
    Microbiology 142:3103-3111(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168 / JH642.
  3. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  4. "SpoIIAB is an anti-sigma factor that binds to and inhibits transcription by regulatory protein sigma F from Bacillus subtilis."
    Duncan L., Losick R.
    Proc. Natl. Acad. Sci. U.S.A. 90:2325-2329(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Sigma F, the first compartment-specific transcription factor of B. subtilis, is regulated by an anti-sigma factor that is also a protein kinase."
    Min K.-T., Hilditch C.M., Diederich B., Errington J., Yudkin M.D.
    Cell 74:735-742(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PROTEIN SEQUENCE OF 1-14.
    Strain: CU267.

Entry nameiSP2AB_BACSU
AccessioniPrimary (citable) accession number: P10728
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: July 9, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

External Data

Dasty 3

Similar proteinsi