Reviewed, UniProtKB/Swiss-Prot P10980 (ACM2_RAT)

Names and origin

Protein names

Recommended name:
    Muscarinic acetylcholine receptor M2

Gene names

Name:	Chrm2
Synonyms:	Chrm-2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	466 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition.
Subcellular location	Cell membrane; Multi-pass membrane protein. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell junction Cell membrane Membrane Postsynaptic cell membrane Synapse
Domain	Transmembrane
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein Phosphoprotein
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 466	466	Muscarinic acetylcholine receptor M2
Regions
	Topological domain	1 – 22	22	Extracellular Potential
	Transmembrane	23 – 45	23	1 Potential
	Topological domain	46 – 59	14	Cytoplasmic Potential
	Transmembrane	60 – 80	21	2 Potential
	Topological domain	81 – 97	17	Extracellular Potential
	Transmembrane	98 – 119	22	3 Potential
	Topological domain	120 – 139	20	Cytoplasmic Potential
	Transmembrane	140 – 162	23	4 Potential
	Topological domain	163 – 184	22	Extracellular Potential
	Transmembrane	185 – 207	23	5 Potential
	Topological domain	208 – 388	181	Cytoplasmic Potential
	Transmembrane	389 – 409	21	6 Potential
	Topological domain	410 – 423	14	Extracellular Potential
	Transmembrane	424 – 443	20	7 Potential
	Topological domain	444 – 466	23	Cytoplasmic Potential
Amino acid modifications
	Modified residue	232	1	Phosphoserine By similarity
	Modified residue	234	1	Phosphoserine By similarity
	Modified residue	446	1	Phosphothreonine Potential
	Modified residue	450	1	Phosphothreonine Potential
	Modified residue	465	1	Phosphothreonine Potential
	Glycosylation	2	1	N-linked (GlcNAc...) Potential
	Glycosylation	3	1	N-linked (GlcNAc...) Potential
	Glycosylation	6	1	N-linked (GlcNAc...) Potential
	Disulfide bond	96 ↔ 176		By similarity
Experimental info
	Sequence conflict	51	1	N → S in AAA40926. Ref.1
	Sequence conflict	273	1	N → T in AAA40926. Ref.1
	Sequence conflict	289 – 290	2	VS → SA in AAA40926. Ref.1
	Sequence conflict	313	1	G → D in AAA40926. Ref.1
	Sequence conflict	337	1	C → Y in AAA40926. Ref.1
	Sequence conflict	353	1	N → S in AAA40926. Ref.1
	Sequence conflict	360	1	I → V in AAA40926. Ref.1
	Sequence conflict	369	1	T → P in AAA40926. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P10980-1 [UniParc]. Last modified December 1, 2000. Version 2. Checksum: 70ECCD86366A676B	FASTA	466	51,539
	10 20 30 40 50 60 MNNSTNSSNN GLAITSPYKT FEVVFIVLVA GSLSLVTIIG NILVMVSIKV NRHLQTVNNY 70 80 90 100 110 120 FLFSLACADL IIGVFSMNLY TLYTVIGYWP LGPVVCDLWL ALDYVVSNAS VMNLLIISFD 130 140 150 160 170 180 RYFCVTKPLT YPVKRTTKMA GMMIAAAWVL SFILWAPAIL FWQFIVGVRT VEDGECYIQF 190 200 210 220 230 240 FSNAAVTFGT AIAAFYLPVI IMTVLYWHIS RASKSRIKKE KKEPVANQDP VSPSLVQGRI 250 260 270 280 290 300 VKPNNNNMPG GDGGLEHNKI QNGKAPRDGV TENCVQGEEK ESSNDSTSVS AVASNMRDDE 310 320 330 340 350 360 ITQDENTVST SLGHSRDDNS KQTCIKIVTK AQKGDVCTPT STTVELVGSS GQNGDEKQNI 370 380 390 400 410 420 VARKIVKMTK QPAKKKPPPS REKKVTRTIL AILLAFIITW APYNVMVLIN TFCAPCIPNT 430 440 450 460 VWTIGYWLCY INSTINPACY ALCNATFKKT FKHLLMCHYK NIGATR

References

[1]

"Primary structure of rat cardiac beta-adrenergic and muscarinic cholinergic receptors obtained by automated DNA sequence analysis: further evidence for a multigene family." Gocayne J.D., Robinson D.A., Fitzgerald M.G., Chung F.-Z., Kerlavage A.R., Lentes K.-U., Lai J., Wang C.-D., Fraser C.M., Venter J.C. Proc. Natl. Acad. Sci. U.S.A. 84:8296-8300(1987) [PubMed: 2825184] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

[2]

"Molecular cloning of m3 muscarinic acetylcholine receptor in rat iris." Furuta M., Ohya S., Imaizumi Y., Watanabe M. J. Smooth Muscle Res. 34:111-122(1998) [PubMed: 9972520] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Iris.

Cross-references

Sequence databases
EMBLGenBankDDBJ	J03025 mRNA. Translation: AAA40926.1. AB017655 mRNA. Translation: BAA36838.1.
PIR	JH0197. S10856.
RefSeq	NP_112278.1.
UniGene	Rn.10752 Rn.200974
3D structure databases
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
Genome annotation databases
Ensembl	ENSRNOG00000011928. Rattus norvegicus. [Contig view]
GeneID	81645.
KEGG	rno:81645.
Organism-specific databases
RGD	620023. Chrm2.
Phylogenomic databases
HOVERGEN	P10980.
Gene expression databases
ArrayExpress	P10980.
GermOnline	ENSRNOG00000011928. Rattus norvegicus.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_supfam. IPR001065. Musac_M2_rcpt. IPR000995. Musac_rcpt. [Graphical view]
PANTHER	PTHR19266:SF32. Musac_M2_rcpt. 1 hit.
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR00243. MUSCARINICR. PR00539. MUSCRINICM2R.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProDom	P10980. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

ACM2_RAT

Accession

Primary (citable) accession number: P10980
Secondary accession number(s): Q9Z2Z1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	December 1, 2000
Last modified:	July 22, 2008
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)