Reviewed, UniProtKB/Swiss-Prot P13022 (CAPZA_DICDI)

Names and origin

Protein names

Recommended name:
    F-actin-capping protein subunit alpha
Alternative name(s):
    Aginactin subunit alpha
    CAP34

Gene names

Name:	acpB
Synonyms:	abpD
ORF Names:	DDB_0191243

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	281 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments.
Subunit structure	Heterodimer of an alpha and a beta subunit.
Sequence similarities	Belongs to the F-actin-capping protein alpha subunit family.

Ontologies

Keywords
Ligand	Actin-binding
Molecular function	Actin capping
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 281	281	F-actin-capping protein subunit alpha

Sequences

	Sequence		Length	Mass (Da)
	P13022-1 [UniParc]. Last modified January 1, 1990. Version 1. Checksum: 488C23AF378FC2F7	FASTA	281	31,163
	10 20 30 40 50 60 MASNQELVQI ATNFLLNAPP CEFMEVVSDV RALLPSESLL NASAGSTFRE YNTSQMVSVQ 70 80 90 100 110 120 TSKGSALITK EGEISNNEYL DPKNKQVITY DHIKQEVTGE RSASGEIEQD IEQYRAAFDE 130 140 150 160 170 180 EATKYCNEYY PNGVSAVYGT KVSEGIKITV CISTCIYKPN AFYSGRWRSV WTCTFKPGSG 190 200 210 220 230 240 NVTSNGKVQV NVHYFEDGNV QLNTVTQKQT TSPSADAQST AVNAFKAIGK AELNLHTALD 250 260 270 280 NNYSTMGDTT FKALRRALPI NRTKINWQKV KNFKIANELN K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Ca2+-independent F-actin capping proteins. Cap 32/34, a capping protein from Dictyostelium discoideum, does not share sequence homologies with known actin-binding proteins." Hartmann H., Noegel A.A., Eckerskorn C., Rapp S., Schleicher M. J. Biol. Chem. 264:12639-12647(1989) [PubMed: 2663863] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
[2]	"Heterodimeric capping proteins constitute a highly conserved group of actin-binding proteins." Hartmann H., Schleicher M., Noegel A.A. Dev. Genet. 11:369-376(1990) [PubMed: 1710551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[4]	"A major agonist-regulated capping activity in Dictyostelium is due to the capping protein, cap32/34." Eddy R.J., Han J., Sauterer R.A., Condeelis J.S. Biochim. Biophys. Acta 1314:247-259(1996) [PubMed: 8982279] [Abstract] Cited for: FUNCTION.
[5]	"Identification and isolation of Dictyostelium microtubule-associated protein interactors by tandem affinity purification." Koch K.V., Reinders Y., Ho T.-H., Sickmann A., Graef R. Eur. J. Cell Biol. 85:1079-1090(2006) [PubMed: 16782229] [Abstract] Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M25132 Genomic DNA. Translation: AAA33176.1. AAFI02000008 Genomic DNA. Translation: EAL71204.1.
PIR	B61042.
RefSeq	XP_645243.1.
3D structure databases
HSSP	HSSP built from PDB template 1IZN based on UniProtKB P13127.
ModBase	Search...
Genome annotation databases
GeneID	3396276.
KEGG	ddi:DDB_0191243.
Organism-specific databases
dictyBase	DDB0191243. acpB.
Family and domain databases
InterPro	IPR002189. F-actin_cap_A. [Graphical view]
PANTHER	PTHR10653. F-actin_cap_A. 1 hit.
Pfam	PF01267. F-actin_cap_A. 1 hit. [Graphical view]
PRINTS	PR00191. FACTINCAPA.
ProDom	PD006960. F-actin_cap_A. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00748. F_ACTIN_CAPPING_A_1. 1 hit. PS00749. F_ACTIN_CAPPING_A_2. 1 hit. [Graphical view]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

CAPZA_DICDI

Accession

Primary (citable) accession number: P13022
Secondary accession number(s): Q559U1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	July 22, 2008
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)