Reviewed, UniProtKB/Swiss-Prot P13298 (PYRE_YEAST)

Names and origin

Protein names

Recommended name:
    Orotate phosphoribosyltransferase 1
      Short name=OPRTase 1
      Short name=OPRT 1
    EC=2.4.2.10

Gene names

Name:	URA5
Synonyms:	PYR5
Ordered Locus Names:	YML106W
ORF Names:	YM8339.13

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	226 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribosyl-1-pyrophosphate (PRPP) to orotate, leading to the formation of orotidine monophosphate (OMP) By similarity.
Catalytic activity	Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 5/6.
Subunit structure	Homodimer By similarity.
Miscellaneous	There are two genes coding for OPRT in yeast. Present with 39300 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Molecular function	Glycosyltransferase Transferase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from mutant phenotype. Source: SGD pyrimidine ribonucleoside biosynthetic process Inferred from direct assay. Source: SGD
Cellular component	cytoplasm Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD
Molecular function	orotate phosphoribosyltransferase activity Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 226	226	Orotate phosphoribosyltransferase 1
Regions
	Region	38 – 39	2	Orotate binding By similarity
	Region	76 – 77	2	PRPP binding By similarity
	Region	132 – 140	9	PRPP binding By similarity
Sites
	Binding site	30	1	PRPP By similarity
	Binding site	106	1	PRPP; shared with dimeric partner By similarity
	Binding site	107	1	PRPP By similarity
	Binding site	110	1	PRPP; shared with dimeric partner By similarity
	Binding site	112	1	PRPP; shared with dimeric partner By similarity
	Binding site	136	1	Orotate By similarity
	Binding site	164	1	Orotate By similarity
Experimental info
	Sequence conflict	150	1	N → S in CAA32901. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P13298-1 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: ECF3A01EB35BC03F	FASTA	226	24,664
	10 20 30 40 50 60 MPIMLEDYQK NFLELAIECQ ALRFGSFKLK SGRESPYFFN LGLFNTGKLL SNLATAYAIA 70 80 90 100 110 120 IIQSDLKFDV IFGPAYKGIP LAAIVCVKLA EIGGSKFQNI QYAFNRKEAK DHGEGGIIVG 130 140 150 160 170 180 SALENKRILI IDDVMTAGTA INEAFEIISN AKGQVVGSII ALDRQEVVST DDKEGLSATQ 190 200 210 220 TVSKKYGIPV LSIVSLIHII TYLEGRITAE EKSKIEQYLQ TYGASA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and expression of the URA5 gene of Saccharomyces cerevisiae." de Montigny J., Belarbi A., Hubert J.-C., Lacroute F. Mol. Gen. Genet. 215:455-462(1989) [PubMed: 2651891] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Stirling C.J. Submitted (FEB-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 13-226.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X14795 Genomic DNA. Translation: CAA32901.1.
Z49210 Genomic DNA. Translation: CAA89112.1.
AY693160 Genomic DNA. Translation: AAT93179.1.
X65783 Genomic DNA. Translation: CAA46665.1.

PIR

XJBY5. S53966.

RefSeq

NP_013601.1.

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2PRY	X-ray	2.35	A	1-226	[»]
2PRZ	X-ray	1.90	A/B/C/D	1-226	[»]
2PS1	X-ray	1.75	A/B	1-226	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:4859N.

IntAct

P13298.

Proteomic databases

PeptideAtlas

P13298.

Genome annotation databases

Ensembl

YML106W. Saccharomyces cerevisiae. [Contig view]

GeneID

854865.

GenomeReviews

Gene locus YML106W in contig Z71257_GR.

KEGG

sce:YML106W.

NMPDR

fig|4932.3.peg.4637.

Organism-specific databases

CYGD

YML106w.

SGD

S000004574. URA5.

Yeast-GFP

Search...

Phylogenomic databases

HOGENOM

P13298.

Gene expression databases

ArrayExpress

P13298.

GermOnline

YML106W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR004467. Or_phspho_trans.
IPR002375. Pr/py_Pribosyl_transf_CS.
IPR000836. PRibTrfase.
[Graphical view]

Pfam

PF00156. Pribosyltran. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00336. pyrE. 1 hit.

PROSITE

PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

ProDom

P13298.
[Graphical view] [Entries sharing at least one domain]

BLOCKS

Search...

Other Resources

LinkHub

P13298.

ProtoNet

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Entry information

Entry name

PYRE_YEAST

Accession

Primary (citable) accession number: P13298

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	February 1, 1996
Last modified:	July 22, 2008
This is version 81 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)