Reviewed, UniProtKB/Swiss-Prot P13640 (MT1G_HUMAN)

Names and origin

Protein names

Recommended name:
    Metallothionein-1G
      Short name(s)=MT-1G
Alternative name(s):
    Metallothionein-IG
      Short name(s)=MT-IG
    Metallothionein-1K
      Short name(s)=MT-1K

Gene names

Name:	MT1G
Synonyms:	MT1K, MT1M

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	62 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.
Subunit structure	Monomer.
Domain	Class I metallothioneins contain 2 metal-binding domains: four divalent ions are chelated within cluster A of the alpha domain and are coordinated via cysteinyl thiolate bridges to 11 cysteine ligands. Cluster B, the corresponding region within the beta domain, can ligate three divalent ions to 9 cysteines.
Sequence similarities	Belongs to the metallothionein superfamily. Type 1 family.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	Cadmium Copper Metal-binding Metal-thiolate cluster Zinc
PTM	Acetylation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SPINK7	P58062	3	EBI-1182473,EBI-1182445

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P13640-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P13640-2)

The sequence of this isoform differs from the canonical sequence as follows:
     10-10: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 62	62	Metallothionein-1G
Regions
	Region	1 – 29	29	Beta
	Region	31 – 62	32	Alpha
Sites
	Metal binding	5	1	Cluster B
	Metal binding	7	1	Cluster B
	Metal binding	14	1	Cluster B
	Metal binding	16	1	Cluster B
	Metal binding	20	1	Cluster B
	Metal binding	22	1	Cluster B
	Metal binding	25	1	Cluster B
	Metal binding	27	1	Cluster B
	Metal binding	30	1	Cluster B
	Metal binding	34	1	Cluster A
	Metal binding	35	1	Cluster A
	Metal binding	37	1	Cluster A
	Metal binding	38	1	Cluster A
	Metal binding	42	1	Cluster A
	Metal binding	45	1	Cluster A
	Metal binding	49	1	Cluster A
	Metal binding	51	1	Cluster A
	Metal binding	58	1	Cluster A
	Metal binding	60	1	Cluster A
	Metal binding	61	1	Cluster A
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine
Natural variations
	Alternative sequence	10	1	Missing in isoform 2.

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 7, 2006. Version 2. Checksum: A1B390F5899DD040	FASTA	62	6,141
	10 20 30 40 50 60 MDPNCSCAAA GVSCTCASSC KCKECKCTSC KKSCCSCCPV GCAKCAQGCI CKGASEKCSC CA
	Isoform 2 [UniParc]. Checksum: 7B0A61FF89CA94CC		61	6,070
	10 20 30 40 50 60 MDPNCSCAAG VSCTCASSCK CKECKCTSCK KSCCSCCPVG CAKCAQGCIC KGASEKCSCC A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and expression of the human metallothionein-IG gene. Differential promoter activity of two linked metallothionein-I genes in response to heavy metals." Foster R., Jahroudi N., Varshney U., Gedamu L. J. Biol. Chem. 263:11528-11535(1988) [PubMed: 3403543] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Structure and expression of the human metallothionein genes." Gedamu L., Varshney U., Jahroudi N., Foster R., Shworak N.W. Experientia Suppl. 52:361-372(1987) [PubMed: 2444457] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	Hunziker P.E. Submitted (NOV-1993) to UniProtKB Cited for: PROTEIN SEQUENCE (ISOFORM 1). Tissue: Liver.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Liver.
[5]	"Induction by zinc of specific metallothionein isoforms in human monocytes." Pauwels M., van Weyenbergh J., Soumillion A., Proost P., Ley M. Eur. J. Biochem. 220:105-110(1994) [PubMed: 8119276] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-31 (ISOFORM 2).

Cross-references

Sequence databases
EMBLGenBankDDBJ	J03910 Genomic DNA. Translation: AAA59873.1. BC020757 mRNA. Translation: AAH20757.1. S68954 mRNA. Translation: AAB30083.1.
PIR	SMHU1G. A29236.
UniGene	Hs.433391
3D structure databases
HSSP	HSSP built from PDB template 1MHU based on UniProtKB P02795.
ModBase	Search...
Protein-protein interaction databases
IntAct	P13640.
Genome annotation databases
Ensembl	ENSG00000125144. Homo sapiens. [Contig view]
Organism-specific databases
H-InvDB	HIX0021664.
HGNC	HGNC:7399. MT1G.
MIM	156353. gene.
PharmGKB	PA31204.
GenAtlas	Search...
GeneCards	Search...
GeneLynx	Search...
Phylogenomic databases
HOGENOM	P13640.
HOVERGEN	P13640.
Gene expression databases
ArrayExpress	P13640.
CleanEx	HS_MT1G. HS_MT1M.
GermOnline	ENSG00000125144. Homo sapiens.
Family and domain databases
InterPro	IPR003019. Metallothionein_sfam_euk. IPR000006. Metallothionein_vert. [Graphical view]
Gene3D	G3DSA:4.10.10.10. Metallothionein_vert. 1 hit.
PANTHER	PTHR23299. Metallothionein_vert. 1 hit.
Pfam	PF00131. Metallothio. 1 hit. [Graphical view]
PRINTS	PR00860. MTVERTEBRATE.
PROSITE	PS00203. METALLOTHIONEIN_VRT. 1 hit. [Graphical view]
ProDom	P13640. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
SOURCE	Search...
ProtoNet	Search...

Entry information

Entry name

MT1G_HUMAN

Accession

Primary (citable) accession number: P13640
Secondary accession number(s): P80296

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	February 7, 2006
Last modified:	July 22, 2008
This is version 74 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)