Reviewed, UniProtKB/Swiss-Prot P13833 (MLR_DICDI)

Names and origin

Protein names

Recommended name:
    Myosin regulatory light chain
Alternative name(s):
    RMLC

Gene names

Name:	mlcR
Synonyms:	mlcA
ORF Names:	DDB_0185146

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	161 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Subunit structure	Myosin is a hexamer of 2 heavy chains and 4 light chains (two regulatory light chains and two essential light chains).
Miscellaneous	The sequences of all three RMLC calcium-binding domains have diverged significantly from that of calmodulin. Therefore, it seems unlikely that these light chains bind calcium. It is potential that phosphorylation has taken over the role of calcium-binding in the regulation of Dictyostelium myosin. In D.discoideum, cAMP stimulation induces RMLC phosphorylation; this chain is phosphorylated on a serine residue.
Sequence similarities	Contains 3 EF-hand domains.
Sequence caution	The sequence AAS38723.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Domain	Repeat
Molecular function	Motor protein Myosin
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 161	161	Myosin regulatory light chain
Regions
	Domain	20 – 55	36	EF-hand 1
	Domain	56 – 91	36	EF-hand 2
	Domain	93 – 128	36	EF-hand 3
Amino acid modifications
	Modified residue	13	1	Phosphoserine Potential
	Modified residue	14	1	Phosphoserine Potential

Sequences

	Sequence		Length	Mass (Da)
	P13833-1 [UniParc]. Last modified January 1, 1990. Version 1. Checksum: 354B704B20F4E2C3	FASTA	161	18,323
	10 20 30 40 50 60 MASTKRRLNR EESSVVLGEE QVAELKEAFE LFDKDRTGFI KKDALKTTCK QFGVFVMEDQ 70 80 90 100 110 120 LDAMFAEADT TKSGAIGFPE FMSMMSRRMK QTSNEQILMN AFKTFDPEGN GYILTKDLSK 130 140 150 160 ALTTLGDKLT EAELQELLSI SENEQKQVKY DLFVNTLFSK K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Dictyostelium discoideum myosin: isolation and characterization of cDNAs encoding the regulatory light chain." Tafuri S.R., Rushforth A.M., Kuczmarski E.R., Chisholm R.L. Mol. Cell. Biol. 9:3073-3080(1989) [PubMed: 2550795] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed: 12097910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M25251 mRNA. Translation: AAA33226.1. AC116979 Genomic DNA. Translation: AAS38723.1. Sequence problems. AAFI02000014 Genomic DNA. Translation: EAL69338.1.
PIR	A30938. A32496.
RefSeq	XP_643313.1.
3D structure databases
HSSP	HSSP built from PDB template 1IWQ based on UniProtKB P02593.
ModBase	Search...
Genome annotation databases
GeneID	3394413.
KEGG	ddi:DDB_0185146.
Organism-specific databases
dictyBase	DDB0185146. mlcR.
Family and domain databases
InterPro	IPR011992. EF-Hand_type. IPR002048. EF_hand_Ca_bd. [Graphical view]
Gene3D	G3DSA:1.10.238.10. EF-Hand_type. 1 hit.
Pfam	PF00036. efhand. 3 hits. [Graphical view]
ProDom	PD000012. EF-hand. 2 hits. [Graphical view] [Entries sharing at least one domain]
SMART	SM00054. EFh. 3 hits. [Graphical view]
PROSITE	PS50222. EF_HAND_2. 3 hits. [Graphical view]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

MLR_DICDI

Accession

Primary (citable) accession number: P13833
Secondary accession number(s): Q552B5, Q75JL0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	July 22, 2008
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)