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Reviewed, UniProtKB/Swiss-Prot P14925 (AMD_RAT)

Last modified July 22, 2008. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Peptidyl-glycine alpha-amidating monooxygenase
      Short name=PAM
Including the following 2 domains:
    1- Recommended name:
            Peptidylglycine alpha-hydroxylating monooxygenase
                Short name=PHM
              EC=1.14.17.3
    2- Recommended name:
            Peptidyl-alpha-hydroxyglycine alpha-amidating lyase
              EC=4.3.2.5
        Alternative name(s):
            Peptidylamidoglycolate lyase
              Short name=PAL
Gene names
Name: Pam
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length976 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Bifunctional enzyme that catalyzes 2 sequencial steps in C-terminal alpha-amidation of peptides. The monooxygenase part produces an unstable peptidyl(2-hydroxyglycine) intermediate that is dismutated to glyoxylate and the corresponding desglycine peptide amide by the lyase part. C-terminal amidation of peptides such as neuropeptides is essential for full biological activity.

Catalytic activity

Peptidylglycine + ascorbate + O(2) = peptidyl(2-hydroxyglycine) + dehydroascorbate + H(2)O.

Peptidylamidoglycolate = peptidyl amide + glyoxylate.

Cofactor

Zinc; for the lyase reaction.

Binds 2 copper ions per subunit; For the monoxygenase reaction.

Subunit structure

Monomer. Interacts with RASSF9.

Subcellular location

Cytoplasmic vesicle › secretory vesicle membrane; Single-pass membrane protein. Note= Secretory granules.

Sequence similarities

In the C-terminal section; belongs to the peptidyl-alpha-hydroxyglycine alpha-amidating lyase family.

In the N-terminal section; belongs to the copper type II ascorbate-dependent monooxygenase family.

Contains 5 NHL repeats.

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Binary interactions

With

Entry

#Exp.

IntAct

Notes

PamciO888691EBI-1395008,EBI-1395057

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Alternative products

This entry describes 7 isoforms produced by alternative splicing. [Align] [Select]
Isoform PAM-1 (identifier: P14925-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Notes: Membrane-bound.
Isoform PAM-2 (identifier: P14925-2)

The sequence of this isoform differs from the canonical sequence as follows:
     393-497: Missing.
Notes: Membrane-bound.
Isoform PAM-3 (identifier: P14925-3)

The sequence of this isoform differs from the canonical sequence as follows:
     393-497: Missing.
     832-917: Missing.
Notes: Soluble.
Isoform PAM-3A (identifier: P14925-4)

The sequence of this isoform differs from the canonical sequence as follows:
     393-497: Missing.
     832-899: Missing.
Notes: Soluble.
Isoform PAM-3B (identifier: P14925-5)

The sequence of this isoform differs from the canonical sequence as follows:
     393-497: Missing.
     900-917: Missing.
Notes: Membrane-bound.
Isoform PAM-4 (identifier: P14925-6)

The sequence of this isoform differs from the canonical sequence as follows:
     498-517: DFHVEEELDWPGVYLLPGQV → GASRISFTQKKKCVKHCNPH
     518-917: Missing.
Notes: Soluble.
Isoform PAM-5 (identifier: P14925-7)

The sequence of this isoform differs from the canonical sequence as follows:
     308-312: GTSSD → FKDTF
     313-976: Missing.
Notes: Soluble.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical view

Molecule processing

Signal peptide1 – 2525
Propeptide26 – 3510
Chain36 – 976941Peptidyl-glycine alpha-amidating monooxygenase

Regions

Topological domain36 – 866831Intragranular Potential
Transmembrane867 – 89024 Potential
Topological domain891 – 97686Cytoplasmic Potential
Repeat501 – 54444NHL 1
Repeat570 – 61142NHL 2
Repeat620 – 66546NHL 3
Repeat673 – 71745NHL 4
Repeat769 – 81244NHL 5
Region1 – 497497Peptidylglycine alpha-hydroxylating monooxygenase
Region498 – 820323Peptidyl-alpha-hydroxyglycine alpha-amidating lyase
Region928 – 94518Interaction with RASSF9

Sites

Metal binding1071Copper A
Metal binding1081Copper A
Metal binding1721Copper A
Metal binding2421Copper B
Metal binding2441Copper B
Metal binding3141Copper B

Amino acid modifications

Modified residue9211Phosphoserine By similarity
Modified residue9611Phosphoserine By similarity
Modified residue9651Sulfotyrosine By similarity
Glycosylation7651N-linked (GlcNAc...)
Disulfide bond47 ↔ 186
Disulfide bond81 ↔ 126
Disulfide bond114 ↔ 131
Disulfide bond227 ↔ 334
Disulfide bond293 ↔ 315
Disulfide bond634 ↔ 655
Disulfide bond702 ↔ 713

Natural variations

Alternative sequence308 – 3125GTSSD → FKDTF in isoform PAM-5.
Alternative sequence313 – 976664Missing in isoform PAM-5.
Alternative sequence393 – 497105Missing in isoform PAM-2, isoform PAM-3, isoform PAM-3A and isoform PAM-3B.
Alternative sequence498 – 51720DFHVE…LPGQV → GASRISFTQKKKCVKHCNPH in isoform PAM-4.
Alternative sequence518 – 917400Missing in isoform PAM-4.
Alternative sequence832 – 91786Missing in isoform PAM-3.
Alternative sequence832 – 89968Missing in isoform PAM-3A.
Alternative sequence900 – 91718Missing in isoform PAM-3B.

Experimental info

Sequence conflict9591T → S Ref.3

Secondary structure

1 ......................................................... 976
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
Isoform PAM-1 [UniParc].

Last modified April 1, 1990. Version 1.
Checksum: 7233021BEBEFD9B9

FASTA976108,675
        10         20         30         40         50         60 
MAGRARSGLL LLLLGLLALQ SSCLAFRSPL SVFKRFKETT RSFSNECLGT IGPVTPLDAS 

        70         80         90        100        110        120 
DFALDIRMPG VTPKESDTYF CMSMRLPVDE EAFVIDFKPR ASMDTVHHML LFGCNMPSST 

       130        140        150        160        170        180 
GSYWFCDEGT CTDKANILYA WARNAPPTRL PKGVGFRVGG ETGSKYFVLQ VHYGDISAFR 

       190        200        210        220        230        240 
DNHKDCSGVS VHLTRVPQPL IAGMYLMMSV DTVIPPGEKV VNADISCQYK MYPMHVFAYR 

       250        260        270        280        290        300 
VHTHHLGKVV SGYRVRNGQW TLIGRQNPQL PQAFYPVEHP VDVTFGDILA ARCVFTGEGR 

       310        320        330        340        350        360 
TEATHIGGTS SDEMCNLYIM YYMEAKYALS FMTCTKNVAP DMFRTIPAEA NIPIPVKPDM 

       370        380        390        400        410        420 
VMMHGHHKEA ENKEKSALMQ QPKQGEEEVL EQGDFYSLLS KLLGEREDVH VHKYNPTEKT 

       430        440        450        460        470        480 
ESGSDLVAEI ANVVQKKDLG RSDAREGAEH EEWGNAILVR DRIHRFHQLE STLRPAESRA 

       490        500        510        520        530        540 
FSFQQPGEGP WEPEPSGDFH VEEELDWPGV YLLPGQVSGV ALDSKNNLVI FHRGDHVWDG 

       550        560        570        580        590        600 
NSFDSKFVYQ QRGLGPIEED TILVIDPNNA EILQSSGKNL FYLPHGLSID TDGNYWVTDV 

       610        620        630        640        650        660 
ALHQVFKLDP HSKEGPLLIL GRSMQPGSDQ NHFCQPTDVA VEPSTGAVFV SDGYCNSRIV 

       670        680        690        700        710        720 
QFSPSGKFVT QWGEESSGSS PRPGQFSVPH SLALVPHLDQ LCVADRENGR IQCFKTDTKE 

       730        740        750        760        770        780 
FVREIKHASF GRNVFAISYI PGFLFAVNGK PYFGDQEPVQ GFVMNFSSGE IIDVFKPVRK 

       790        800        810        820        830        840 
HFDMPHDIVA SEDGTVYIGD AHTNTVWKFT LTEKMEHRSV KKAGIEVQEI KEAEAVVEPK 

       850        860        870        880        890        900 
VENKPTSSEL QKMQEKQKLS TEPGSGVSVV LITTLLVIPV LVLLAIVMFI RWKKSRAFGD 

       910        920        930        940        950        960 
HDRKLESSSG RVLGRFRGKG SGGLNLGNFF ASRKGYSRKG FDRVSTEGSD QEKDEDDGTE 

       970 
SEEEYSAPLP KPAPSS

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Isoform PAM-2 [UniParc].

Checksum: BA1F40E92F7B8C02
Show »

87196,825
Isoform PAM-3 [UniParc].

Checksum: D49246033AEA388B
Show »

78587,253