Reviewed, UniProtKB/Swiss-Prot P15391 (CD19_HUMAN)

Names and origin

Protein names

Recommended name:
    B-lymphocyte antigen CD19
Alternative name(s):
    Differentiation antigen CD19
    B-lymphocyte surface antigen B4
    Leu-12
    CD_antigen=CD19

Gene names

Name:

CD19

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	556 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
Subunit structure	Forms a complex with CD21, CD81 and CD225 in the membrane of mature B cells. Interacts with VAV. Interacts with GRB2 and SOS when phosphorylated on Tyr-348 and/or Tyr-378. Interacts with PLCG2 when phosphorylated on Tyr-409.
Subcellular location	Membrane; Single-pass type I membrane protein.
Post-translational modification	Phosphorylated on serine and threonine upon DNA damage, probably by ATM or ATR. Phosphorylated on tyrosine following B-cell activation.
Involvement in disease	Defects in CD19 are a cause of hypogammaglobulinemia [MIM:107265].
Sequence similarities	Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Sequence caution	The sequence AAA35533.1 differs from that shown. Reason: Frameshift at position 396.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Polymorphism
Domain	Immunoglobulin domain Repeat Signal Transmembrane
PTM	Glycoprotein Phosphoprotein
Gene Ontology (GO)
Biological process	cell surface receptor linked signal transduction Traceable author statement. Source: ProtInc cellular defense response Traceable author statement. Source: ProtInc
Cellular component	integral to plasma membrane Ref.2 Traceable author statement. Source: ProtInc
Molecular function	protein binding Inferred from physical interaction. Source: IntAct receptor signaling protein activity Traceable author statement. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PIK3R3	Q92569	1	EBI-79902,EBI-79893

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 556	537	B-lymphocyte antigen CD19
Regions
	Topological domain	20 – 291	272	Extracellular Potential
	Transmembrane	292 – 313	22	Potential
	Topological domain	314 – 556	243	Cytoplasmic Potential
	Domain	20 – 113	94	Ig-like C2-type 1
	Domain	176 – 277	102	Ig-like C2-type 2
Amino acid modifications
	Modified residue	227	1	Phosphoserine By similarity
	Modified residue	348	1	Phosphotyrosine
	Modified residue	378	1	Phosphotyrosine
	Modified residue	409	1	Phosphotyrosine
	Modified residue	439	1	Phosphotyrosine
	Modified residue	497	1	Phosphoserine
	Glycosylation	86	1	N-linked (GlcNAc...) Potential
	Glycosylation	125	1	N-linked (GlcNAc...) Potential
	Glycosylation	138	1	N-linked (GlcNAc...) Potential
	Glycosylation	181	1	N-linked (GlcNAc...) Potential
	Glycosylation	265	1	N-linked (GlcNAc...) Potential
	Disulfide bond	38 ↔ 97		Potential
	Disulfide bond	200 ↔ 261		Potential
Natural variations
	Natural variant	174	1	L → V: dbSNP rs2904880.
	Natural variant	514	1	R → H: dbSNP rs34763945.
Experimental info
	Sequence conflict	29	1	E → EG in AAB60697. Ref.4
	Sequence conflict	80	1	I → S Ref.2 Ref.3 Ref.4 Ref.5
	Sequence conflict	186	1	Q → QAFLVLSLPVP Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P15391-1 [UniParc]. Last modified November 13, 2007. Version 6. Checksum: A0E08DD628B69E51	FASTA	556	61,128
	10 20 30 40 50 60 MPPPRLLFFL LFLTPMEVRP EEPLVVKVEE GDNAVLQCLK GTSDGPTQQL TWSRESPLKP 70 80 90 100 110 120 FLKLSLGLPG LGIHMRPLAI WLFIFNVSQQ MGGFYLCQPG PPSEKAWQPG WTVNVEGSGE 130 140 150 160 170 180 LFRWNVSDLG GLGCGLKNRS SEGPSSPSGK LMSPKLYVWA KDRPEIWEGE PPCLPPRDSL 190 200 210 220 230 240 NQSLSQDLTM APGSTLWLSC GVPPDSVSRG PLSWTHVHPK GPKSLLSLEL KDDRPARDMW 250 260 270 280 290 300 VMETGLLLPR ATAQDAGKYY CHRGNLTMSF HLEITARPVL WHWLLRTGGW KVSAVTLAYL 310 320 330 340 350 360 IFCLCSLVGI LHLQRALVLR RKRKRMTDPT RRFFKVTPPP GSGPQNQYGN VLSLPTPTSG 370 380 390 400 410 420 LGRAQRWAAG LGGTAPSYGN PSSDVQADGA LGSRSPPGVG PEEEEGEGYE EPDSEEDSEF 430 440 450 460 470 480 YENDSNLGQD QLSQDGSGYE NPEDEPLGPE DEDSFSNAES YENEDEELTQ PVARTMDFLS 490 500 510 520 530 540 PHGSAWDPSR EATSLGSQSY EDMRGILYAA PQLRSIRGQP GPNHEEDADS YENMDNPDGP 550 DPAWGGGGRM GTWSTR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"CD19, the earliest differentiation antigen of the B cell lineage, bears three extracellular immunoglobulin-like domains and an Epstein-Barr virus-related cytoplasmic tail." Stamenkovic I., Seed B. J. Exp. Med. 168:1205-1210(1988) [PubMed: 2459292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS VAL-174 AND HIS-514.
[2]	"Isolation of cDNAs encoding the CD19 antigen of human and mouse B lymphocytes. A new member of the immunoglobulin superfamily." Tedder T.F., Isaacs C.M. J. Immunol. 143:712-717(1989) [PubMed: 2472450] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS VAL-174 AND HIS-514. Tissue: Tonsil.
[3]	"The promoter of the CD19 gene is a target for the B-cell-specific transcription factor BSAP." Kozmik Z., Wang S., Doerfler P., Adams B., Busslinger M. Mol. Cell. Biol. 12:2662-2672(1992) [PubMed: 1375324] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT VAL-174.
[4]	"Structure of the genes encoding the CD19 antigen of human and mouse B lymphocytes." Zhou L.J., Ord D.C., Omori S.A., Tedder T.F. Immunogenetics 35:102-111(1992) [PubMed: 1370948] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT VAL-174. Tissue: Blood.
[5]	"Polymorphisms of human CD19 gene: possible association with susceptibility to systemic lupus erythematosus in Japanese." Kuroki K., Tsuchiya N., Tsao B.P., Grossman J.M., Fukazawa T., Hagiwara K., Kano H., Takazoe M., Iwata T., Hashimoto H., Tokunaga K. Genes Immun. 3:S21-S30(2002) [PubMed: 12215898] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT VAL-174.
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: B-cell.
[7]	"Tyrosine phosphorylation of CD19 in pre-B and mature B cells." Chalupny N.J., Kanner S.B., Schieven G.L., Wee S., Gilliland L.K., Aruffo A., Ledbetter J.A. EMBO J. 12:2691-2696(1993) [PubMed: 7687539] [Abstract] Cited for: PHOSPHORYLATION.
[8]	"Systematic analysis of the role of CD19 cytoplasmic tyrosines in enhancement of activation in Daudi human B cells: clustering of phospholipase C and Vav and of Grb2 and Sos with different CD19 tyrosines." Brooks S.R., Li X., Volanakis E.J., Carter R.H. J. Immunol. 164:3123-3131(2000) [PubMed: 10706702] [Abstract] Cited for: INTERACTION WITH GRB2; SOS; VAV AND PLCG2, PHOSPHORYLATION AT TYR-348; TYR-378; TYR-409 AND TYR-439.
[9]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-497, MASS SPECTROMETRY.
[10]	"An antibody-deficiency syndrome due to mutations in the CD19 gene." van Zelm M.C., Reisli I., van der Burg M., Castano D., van Noesel C.J.M., van Tol M.J.D., Woellner C., Grimbacher B., Patino P.J., van Dongen J.J.M., Franco J.L. N. Engl. J. Med. 354:1901-1912(2006) [PubMed: 16672701] [Abstract] Cited for: INVOLVEMENT IN HYPOGAMMAGLOBULINEMIA.
+	Additional computationally mapped references.

Web resources

CD19base

CD19 mutation db

GeneReviews

Cross-references

Sequence databases
EMBLGenBankDDBJ	M21097 mRNA. Translation: AAA35533.1. Frameshift. M28170 mRNA. Translation: AAA68490.1. M84371 Genomic DNA. Translation: AAA69966.1. M62550 , M62544, M62545, M62546, M62547, M62548, M62549 Genomic DNA. Translation: AAB60697.1. AB052799 Genomic DNA. Translation: BAB60954.1. BC006338 mRNA. Translation: AAH06338.1.
PIR	A44441.
RefSeq	NP_001761.3.
UniGene	Hs.652262
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P15391.
PTM databases
PhosphoSite	P15391.
Genome annotation databases
Ensembl	ENSG00000177455. Homo sapiens. [Contig view]
GeneID	930.
KEGG	hsa:930.
Organism-specific databases
HGNC	HGNC:1633. CD19.
HPA	CAB016110.
MIM	107265. gene+phenotype.
Orphanet	77303. Hypogammaglobulinemia due to CD19 deficiency.
PharmGKB	PA26192.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOGENOM	P15391.
HOVERGEN	P15391.
Enzyme and pathway databases
Reactome	REACT_6900. Signaling in Immune System.
Gene expression databases
ArrayExpress	P15391.
CleanEx	HS_CD19.
GermOnline	ENSG00000177455. Homo sapiens.
Family and domain databases
InterPro	IPR007110. Ig-like. IPR003599. Ig_sub. [Graphical view]
SMART	SM00409. IG. 2 hits. [Graphical view]
PROSITE	PS50835. IG_LIKE. 2 hits. [Graphical view]
ProDom	P15391. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
SOURCE	Search...
ProtoNet	Search...

Entry information

Entry name

CD19_HUMAN

Accession

Primary (citable) accession number: P15391
Secondary accession number(s): Q96S68, Q9BRD6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	November 13, 2007
Last modified:	July 22, 2008
This is version 94 of the entry and version 6 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)