Reviewed, UniProtKB/Swiss-Prot P15625 (SYFA_YEAST)

Names and origin

Protein names

Recommended name:
    Phenylalanyl-tRNA synthetase alpha chain
    EC=6.1.1.20
Alternative name(s):
    Phenylalanine--tRNA ligase alpha chain
      Short name=PheRS

Gene names

Name:	FRS2
Ordered Locus Names:	YFL022C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	503 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe).
Subunit structure	Tetramer of two alpha and two beta chains.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 2 subfamily.
Caution	Was originally (Ref.1) erroneously assigned as a beta chain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	phenylalanyl-tRNA aminoacylation Ref.1 Traceable author statement. Source: SGD
Cellular component	phenylalanine-tRNA ligase complex Ref.1 Inferred from direct assay. Source: SGD
Molecular function	phenylalanine-tRNA ligase activity Ref.1 Inferred from mutant phenotype. Source: SGD protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
FRS1	P15624	1	EBI-18678,EBI-18684

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 503	502	Phenylalanyl-tRNA synthetase alpha chain
Regions
	Region	2 – 173	172	Contains the major tRNA-Phe binding sites
Amino acid modifications
	Modified residue	2	1	N-acetylserine
	Modified residue	39	1	Phosphoserine
	Modified residue	117	1	Phosphoserine
Experimental info
	Sequence conflict	177	1	N → S in AAA35152. Ref.1
	Sequence conflict	289	1	D → E in AAA35152. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P15625-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 50B7EAC675AB559B	FASTA	503	57,511
	10 20 30 40 50 60 MSDFQLEILK KLDELDEIKS TLATFPQHGS QDVLSALNSL KAHNKLEFSK VDTVTYDLTK 70 80 90 100 110 120 EGAQILNEGS YEIKLVKLIQ ELGQLQIKDV MSKLGPQVGK VGQARAFKNG WIAKNASNEL 130 140 150 160 170 180 ELSAKLQNTD LNELTDETQS ILAQIKNNSH LDSIDAKILN DLKKRKLIAQ GKITDFNVTK 190 200 210 220 230 240 GPEFSTDLTK LETDLTSDMV STNAYKDLKF KPYNFNSQGV QISSGALHPL NKVREEFRQI 250 260 270 280 290 300 FFSMGFTEMP SNQYVETGFW NFDALYVPQQ HPARDLQDTF YIKDPLTADL PDDKTYMDNI 310 320 330 340 350 360 KAVHEQGRFG SIGYRYNWKP EECQKLVLRT HSTAISARML HDLAKDPKPT RLFSIDRVFR 370 380 390 400 410 420 NEAVDATHLA EFHQVEGVLA DYNITLGDLI KFMEEFFERM GVTGLRFKPT YNPYTEPSME 430 440 450 460 470 480 IFSWHEGLQK WVEIGNSGMF RPEMLESMGL PKDLRVLGWG LSLERPTMIK YKVQNIRELL 490 500 GHKVSLDFIE TNPAARLDED LYE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and expression of the genes encoding the alpha and beta subunits of yeast phenylalanyl-tRNA synthetase." Sanni A., Mirande M., Ebel J.-P., Boulanger Y., Waller J.-P., Fasiolo F. J. Biol. Chem. 263:15407-15415(1988) [PubMed: 3049607] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
[2]	"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed: 7670463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Identification of the major tRNA(Phe) binding domain in the tetrameric structure of cytoplasmic phenylalanyl-tRNA synthetase from baker's yeast." Fasiolo F., Sanni A., Potier S., Ebel J.-P., Boulanger Y. FEBS Lett. 242:351-356(1989) [PubMed: 2644133] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE, DOMAIN TRNA-BINDING.
[4]	"Proteome studies of Saccharomyces cerevisiae: identification and characterization of abundant proteins." Garrels J.I., McLaughlin C.S., Warner J.R., Futcher B., Latter G.I., Kobayashi R., Schwender B., Volpe T., Anderson D.S., Mesquita-Fuentes R., Payne W.E. Electrophoresis 18:1347-1360(1997) [PubMed: 9298649] [Abstract] Cited for: ACETYLATION AT SER-2.
[5]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-39 AND SER-117, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	J03965 Genomic DNA. Translation: AAA35152.1. D50617 Genomic DNA. Translation: BAA09216.1.
PIR	YFBYAC. S56232.
RefSeq	NP_116631.1.
3D structure databases
HSSP	HSSP built from PDB template 1JJC based on UniProtKB P27001.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:5428N.
IntAct	P15625.
Proteomic databases
PeptideAtlas	P15625.
Genome annotation databases
Ensembl	YFL022C. Saccharomyces cerevisiae. [Contig view]
GeneID	850522.
GenomeReviews	Gene locus YFL022C in contig D50617_GR.
KEGG	sce:YFL022C.
NMPDR	fig|4932.3.peg.2258.
Organism-specific databases
CYGD	YFL022c.
SGD	S000001872. FRS2.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P15625.
Gene expression databases
ArrayExpress	P15625.
GermOnline	YFL022C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR006195. aa-tRNA-synth_II. IPR002319. Phe-tRNA-synth_IIc. IPR004529. Phe-tRNA-synth_IIc_asu. [Graphical view]
PANTHER	PTHR11538. tRNA-synt_2d. 1 hit.
Pfam	PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
TIGRFAMs	TIGR00468. pheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProDom	P15625. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
LinkHub	P15625.
ProtoNet	Search...

Entry information

Entry name

SYFA_YEAST

Accession

Primary (citable) accession number: P15625

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	January 23, 2007
Last modified:	September 2, 2008
This is version 90 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)