Reviewed, UniProtKB/Swiss-Prot P16340 (PUR2_DROPS)

Names and origin

Protein names

Recommended name:
    Trifunctional purine biosynthetic protein adenosine-3
Including 3 domains:
  Recommended name:
      Phosphoribosylamine--glycine ligase
      EC=6.3.4.13
  Alternative name(s):
      Glycinamide ribonucleotide synthetase
        Short name(s)=GARS
      Phosphoribosylglycinamide synthetase
  Recommended name:
      Phosphoribosylformylglycinamidine cyclo-ligase
      EC=6.3.3.1
  Alternative name(s):
      Phosphoribosyl-aminoimidazole synthetase
      AIR synthase
        Short name(s)=AIRS
  Recommended name:
      Phosphoribosylglycinamide formyltransferase
      EC=2.1.2.2
  Alternative name(s):
      5'-phosphoribosylglycinamide transformylase
      GAR transformylase
        Short name(s)=GART

Gene names

Name:	ade3
Synonyms:	gart
ORF Names:	GA16345

Organism

Drosophila pseudoobscura (Fruit fly) [Complete proteome]

Taxonomic identifier

7237 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	1364 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole. 10-formyltetrahydrofolate + N(1)-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/5. Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. Purine metabolism; IMP biosynthesis via de novo pathway; N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route): step 1/1.
Sequence similarities	In the N-terminal section; belongs to the GARS family. In the central section; belongs to the AIR synthase family. In the C-terminal section; belongs to the GART family. Contains 1 ATP-grasp domain.
Sequence caution	EAL34461.1 sequence differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Purine biosynthesis
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
None. [Check GOA]

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Long (identifier: P16340-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Short (identifier: P16340-2)

The sequence of this isoform differs from the canonical sequence as follows:
     434-434: I → M
     435-1364: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 1364	1364	Trifunctional purine biosynthetic protein adenosine-3
Regions
	Domain	114 – 321	208	ATP-grasp
	Nucleotide binding	140 – 202	63	ATP By similarity
	Region	435 – 1154	720	AIRS
	Region	1155 – 1364	210	GART
	Region	1166 – 1168	3	5'-phosphoribosylglycinamide binding By similarity
	Region	1246 – 1249	4	10-formyltetrahydrofolate binding By similarity
	Region	1297 – 1301	5	10-formyltetrahydrofolate binding By similarity
	Region	1327 – 1330	4	5'-phosphoribosylglycinamide binding By similarity
Sites
	Active site	1265	1	Proton donor By similarity
	Metal binding	291	1	Manganese By similarity
	Metal binding	293	1	Manganese By similarity
	Binding site	1221	1	10-formyltetrahydrofolate By similarity
	Binding site	1263	1	10-formyltetrahydrofolate By similarity
	Site	1301	1	Raises pKa of active site His By similarity
Natural variations
	Alternative sequence	434	1	I → M in isoform Short.
	Alternative sequence	435 – 1364	930	Missing in isoform Short.
Experimental info
	Sequence conflict	327	1	L → M in AAX12966. Ref.3
	Sequence conflict	425	1	T → M in AAX12966. Ref.3
	Sequence conflict	805	1	T → S in CAA29611. Ref.1
	Sequence conflict	956	1	G → A in CAA29611. Ref.1
	Sequence conflict	1145	1	A → T in CAA29611. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform Long [UniParc]. Last modified June 27, 2006. Version 2. Checksum: 4BDF1C7BD435E642	FASTA	1,364	145,663
	10 20 30 40 50 60 MSHSVLVIGS GGREHAICWK LSQSTLVKQI YALPGSFGIQ QVEKCRNLDA KVLDPKDFEA 70 80 90 100 110 120 IAKWSKKNEI SLVVVGPEDP LALGLGDVLQ KEGIPCFGPG KQGAQIEADK KWAKDFMLRH 130 140 150 160 170 180 GIPTARYESF TDTNKAKAFI RSAPYQALVV KAAGLAAGKG VVVAANVDEA CQAVDEILGD 190 200 210 220 230 240 LKYGQAGATL VVEELLEGEE ISVLAFTDGK SVRAMLPAQD HKRLGNGDTG PNTGGMGAYC 250 260 270 280 290 300 PCPLISQPAL ELVQRAVLER AVQGLIKERI TYQGVLYAGL MLTRDGPRVL EFNCRFGDPE 310 320 330 340 350 360 TQVILPLLET DLFEVMQACC SGQLDRLPLQ WRSGVSAVGV VLASAGYPET STKGCLITGL 370 380 390 400 410 420 PDVNSPTQLI FHSGLSVNKQ KEALTNGGRV LIAIALDASL KEAAAKATKL AGTITFAGTG 430 440 450 460 470 480 AQYRTDIAQK AFKIAIATAP GLSYKDSGVD IDAGDALVQR IKPLSRGTQR PGVLGGLGGF 490 500 510 520 530 540 GGLFRLKDLS YKEPVIAEAT QGVGAKIQLA LQNELYENIG YDLFAMSAND LLELGAEPVA 550 560 570 580 590 600 FLDYIACGKL HVPLAAQLVK GMADGCRDAK CALVGGETAE MPSLYAPGQH DMAGYCVGIV 610 620 630 640 650 660 EQARVLPRFD LYEPEDLLVG LPSSGLHCAG FNEILTQLAA SKVNLKECSP VGGGKHGLSL 670 680 690 700 710 720 AQVLGTPTRL YVQQLLPHLQ AGNQIKAVAH VTHGLLHDVQ RLLPEGFEVT LDFGAVPVPD 730 740 750 760 770 780 VFGWLAGQLQ LSAQTLLERH NCGIGMVLVL PQSSLLWRTA LPGAKVLGVL NRQAKASGGA 790 800 810 820 830 840 PRVKVRNFVE QLQKLAAPFG GLGETQLPEE VKDVPSSGVK ATTREECFEN AVGRRLTRVP 850 860 870 880 890 900 NHYVDPILIL GTDGVGTKLK IAQQTHRNAS VGIDLVAMCV NDILCNGAEP FSFSSYYACG 910 920 930 940 950 960 KWQAALAAEV NAGVQEGASQ ANSSFVASHS AALPLLYEPQ VYDLAGFALG IAERSGILPR 970 980 990 1000 1010 1020 LDEIQPGDVL IGLPSSGVHS NGFSLVHAVL KRAGLGLNDR APFSEKTLGE ELLVPTKIYV 1030 1040 1050 1060 1070 1080 KALSALLSRP NHGIKALAHI TGGGLSENIP RVLRKELAVR LDANKYPLPP VFAWLAAAGN 1090 1100 1110 1120 1130 1140 ISSTELQRTY NCGLGLVLVV GATEVDGVLR ELRYPQRASV VGEVVARKDP KKPQVVVQNF 1150 1160 1170 1180 1190 1200 EASLARTQRM LSQPRKRVAV LISGKGSNLQ ALIDAIRDSA QGVYAEIVLV ISNKAGVLGL 1210 1220 1230 1240 1250 1260 ERAAKAGIPS MVISHKDFPS REVYDVELTR HLKTARVEFI CLAGFMRILS VPFVREWRGR 1270 1280 1290 1300 1310 1320 LINIHPSLLP KFPGLHVQKQ ALEAGETESG CTVHYVDEGV DTGAIIVQAA VPILPGDDEE 1330 1340 1350 1360 TLTQRIHYAE HWAFPRALAL LASGALRRVS EVKKEAPKDI KDSQ
	Isoform Short [UniParc]. Checksum: B0067502F4A69D52		434	46,252
	10 20 30 40 50 60 MSHSVLVIGS GGREHAICWK LSQSTLVKQI YALPGSFGIQ QVEKCRNLDA KVLDPKDFEA 70 80 90 100 110 120 IAKWSKKNEI SLVVVGPEDP LALGLGDVLQ KEGIPCFGPG KQGAQIEADK KWAKDFMLRH 130 140 150 160 170 180 GIPTARYESF TDTNKAKAFI RSAPYQALVV KAAGLAAGKG VVVAANVDEA CQAVDEILGD 190 200 210 220 230 240 LKYGQAGATL VVEELLEGEE ISVLAFTDGK SVRAMLPAQD HKRLGNGDTG PNTGGMGAYC 250 260 270 280 290 300 PCPLISQPAL ELVQRAVLER AVQGLIKERI TYQGVLYAGL MLTRDGPRVL EFNCRFGDPE 310 320 330 340 350 360 TQVILPLLET DLFEVMQACC SGQLDRLPLQ WRSGVSAVGV VLASAGYPET STKGCLITGL 370 380 390 400 410 420 PDVNSPTQLI FHSGLSVNKQ KEALTNGGRV LIAIALDASL KEAAAKATKL AGTITFAGTG 430 AQYRTDIAQK AFKM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Conserved arrangement of nested genes at the Drosophila Gart locus." Henikoff S., Eghtedarzadeh M.K. Genetics 117:711-725(1987) [PubMed: 3123310] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING. Strain: EST10.
[2]	"Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution." Richards S., Liu Y., Bettencourt B.R., Hradecky P., Letovsky S., Nielsen R., Thornton K., Hubisz M.J., Chen R., Meisel R.P., Couronne O., Hua S., Smith M.A., Zhang P., Liu J., Bussemaker H.J., van Batenburg M.F., Howells S.L. , Scherer S.E., Sodergren E., Matthews B.B., Crosby M.A., Schroeder A.J., Ortiz-Barrientos D., Rives C.M., Metzker M.L., Muzny D.M., Scott G., Steffen D., Wheeler D.A., Worley K.C., Havlak P., Durbin K.J., Egan A., Gill R., Hume J., Morgan M.B., Miner G., Hamilton C., Huang Y., Waldron L., Verduzco D., Clerc-Blankenburg K.P., Dubchak I., Noor M.A.F., Anderson W., White K.P., Clark A.G., Schaeffer S.W., Gelbart W.M., Weinstock G.M., Gibbs R.A. Genome Res. 15:1-18(2005) [PubMed: 15632085] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MV2-25.
[3]	"Patterns of selection on synonymous and nonsynonymous variants in Drosophila miranda." Bartolome C., Maside X., Yi S., Grant A.L., Charlesworth B. Genetics 169:1495-1507(2005) [PubMed: 15545653] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 107-565.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X06285 Genomic DNA. Translation: CAA29611.1. CH379058 Genomic DNA. Translation: EAL34461.1. Sequence problems. AY754390 Genomic DNA. Translation: AAX12966.1.
PIR	AJFFPP. S01204.
RefSeq	XP_001357392.1.
3D structure databases
HSSP	HSSP built from PDB template 1NJS based on UniProtKB P22102.
ModBase	Search...
Genome annotation databases
GeneID	4817813.
KEGG	dpo:Dpse_GA16345.
Organism-specific databases
FlyBase	FBgn0012717. Dpse\ade3.
Family and domain databases
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR002376. Formyl_transf_N. IPR000115. Gars. IPR001555. GART_AS. IPR004733. PurM_cligase. IPR004607. PurN_trans. [Graphical view]
Gene3D	G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.90.600.10. Gars. 1 hit.
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. PF00551. Formyl_trans_N. 1 hit. PF01071. GARS_A. 1 hit. PF02843. GARS_C. 1 hit. PF02844. GARS_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00877. purD. 1 hit. TIGR00878. purM. 2 hits. TIGR00639. PurN. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00184. GARS. 1 hit. PS00373. GART. 1 hit. [Graphical view]
ProDom	P16340. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

PUR2_DROPS

Accession

Primary (citable) accession number: P16340
Secondary accession number(s): Q29P31, Q56RW7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	June 27, 2006
Last modified:	July 22, 2008
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project