Reviewed, UniProtKB/Swiss-Prot P16395 (ACM1_DROME)

Names and origin

Protein names

Recommended name:
    Muscarinic acetylcholine receptor DM1

Gene names

Name:	mAcR-60C
Synonyms:	AcrC
ORF Names:	CG4356

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	805 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover. May have a role in the processing of olfactory and mechanosensory signals; regulation of neurosecretion.
Subcellular location	Cell membrane; Multi-pass membrane protein. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein.
Tissue specificity	Intense staining in the glomeruli of the antennal lobes, the region of the nervous system containing terminals of antennal olfactory sensory neurons and mechanosensory neurons. Also a discrete group of neurosecretory cells in the pars intercerebralis of the brain.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.
Sequence caution	AAA85449.1 sequence differs from that shown. Reason: Frameshift at position 57.

Ontologies

Keywords
Cellular component	Cell junction Cell membrane Membrane Postsynaptic cell membrane Synapse
Coding sequence diversity	Alternative splicing
Domain	Transmembrane
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	plasma membrane Non-traceable author statement. Source: FlyBase
Molecular function	muscarinic acetylcholine receptor activity Inferred from direct assay. Source: FlyBase
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform B (identifier: P16395-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform A (identifier: P16395-2)

The sequence of this isoform differs from the canonical sequence as follows:
     427-443: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 805	805	Muscarinic acetylcholine receptor DM1
Regions
	Topological domain	1 – 100	100	Extracellular Potential
	Transmembrane	101 – 121	21	1 Potential
	Topological domain	122 – 141	20	Cytoplasmic Potential
	Transmembrane	142 – 162	21	2 Potential
	Topological domain	163 – 177	15	Extracellular Potential
	Transmembrane	178 – 198	21	3 Potential
	Topological domain	199 – 220	22	Cytoplasmic Potential
	Transmembrane	221 – 241	21	4 Potential
	Topological domain	242 – 266	25	Extracellular Potential
	Transmembrane	267 – 287	21	5 Potential
	Topological domain	288 – 718	431	Cytoplasmic Potential
	Transmembrane	719 – 739	21	6 Potential
	Topological domain	740 – 752	13	Extracellular Potential
	Transmembrane	753 – 773	21	7 Potential
	Topological domain	774 – 805	32	Cytoplasmic Potential
	Compositional bias	24 – 69	46	Thr-rich
	Compositional bias	502 – 534	33	Asn-rich
Amino acid modifications
	Glycosylation	65	1	N-linked (GlcNAc...) Potential
	Glycosylation	84	1	N-linked (GlcNAc...) Potential
	Glycosylation	87	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	427 – 443	17	Missing in isoform A.
Experimental info
	Sequence conflict	23	1	K → R in AAA28676. Ref.1
	Sequence conflict	155	1	A → T in AAA85449. Ref.4
	Sequence conflict	199	1	S → N in AAA85449. Ref.4
	Sequence conflict	216	1	R → G in AAA85449. Ref.4
	Sequence conflict	227	1	Missing Ref.4
	Sequence conflict	331	1	G → P in AAA85449. Ref.4
	Sequence conflict	355	1	P → A Ref.1 Ref.4
	Sequence conflict	462	1	G → A in AAA28676. Ref.1
	Sequence conflict	532	1	A → T in AAA85449. Ref.4
	Sequence conflict	687 – 688	2	VG → C in AAA85449. Ref.4
	Sequence conflict	696 – 697	2	AR → P in AAA85449. Ref.4
	Sequence conflict	737 – 739	3	VLI → CLS in AAA85449. Ref.4
	Sequence conflict	771	1	C → S in AAA85449. Ref.4
	Sequence conflict	797 – 805	9	EGMVRGVYN → DFMYAASTIR in AAA85449. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform B [UniParc]. Last modified November 23, 2004. Version 2. Checksum: 97A9229CAA5BBED8	FASTA	805	86,623
	10 20 30 40 50 60 MEPVMSLALA AHGPPSILEP LFKTVTTSTT TTTTTTTSTT TTTASPAGYS PGYPGTTLLT 70 80 90 100 110 120 ALFENLTSTA ASGLYDPYSG MYGNQTNGTI GFETKGPRYS LASMVVMGFV AAILSTVTVA 130 140 150 160 170 180 GNVMVMISFK IDKQLQTISN YFLFSLAIAD FAIGAISMPL FAVTTILGYW PLGPIVCDTW 190 200 210 220 230 240 LALDYLASNA SVLNLLIISF DRYFSVTRPL TYRAKRTTNR AAVMIGAAWG ISLLLWPPWI 250 260 270 280 290 300 YSWPYIEGKR TVPKDECYIQ FIETNQYITF GTALAAFYFP VTIMCFLYWR IWRETKKRQK 310 320 330 340 350 360 DLPNLQAGKK DSSKRSNSSD ENTVVNHASG GLLAFAQVGG NDHDTWRRPR SESSPDAESV 370 380 390 400 410 420 YMTNMVIDSG YHGMHSRKSS IKSTNTIKKS YTCFGSIKEW CIAWWHSGRE DSDDFAYEQE 430 440 450 460 470 480 EPSDLGYATP VTIETPLQSS VSRCTSMNVM RDNYSMGGSV SGVRPPSILL SDVSPTPLPR 490 500 510 520 530 540 PPLASISQLQ EMSAVTASTT ANVNTSGNGN GAINNNNNAS HNGNGAVNGN GAGNGSGIGL 550 560 570 580 590 600 GTTGNATHRD SRTLPVINRI NSRSVSQDSV YTILIRLPSD GASSNAANGG GGGPGAGAAA 610 620 630 640 650 660 SASLSMQGDC APSIKMIHED GPTTTAAAAP LASAAATRRP LPSRDSEFSL PLGRRMSHAQ 670 680 690 700 710 720 HDARLLNAKV IPKQLGKAGG GAAGGGVGGA HALMNARNAA KKKKKSQEKR QESKAAKTLS 730 740 750 760 770 780 AILLSFIITW TPYNILVLIK PLTTCSDCIP TELWDFFYAL CYINSTINPM CYALCNATFR 790 800 RTYVRILTCK WHTRNREGMV RGVYN
	Isoform A [UniParc]. Checksum: AD8476D9F52AB522		788	84,792
	10 20 30 40 50 60 MEPVMSLALA AHGPPSILEP LFKTVTTSTT TTTTTTTSTT TTTASPAGYS PGYPGTTLLT 70 80 90 100 110 120 ALFENLTSTA ASGLYDPYSG MYGNQTNGTI GFETKGPRYS LASMVVMGFV AAILSTVTVA 130 140 150 160 170 180 GNVMVMISFK IDKQLQTISN YFLFSLAIAD FAIGAISMPL FAVTTILGYW PLGPIVCDTW 190 200 210 220 230 240 LALDYLASNA SVLNLLIISF DRYFSVTRPL TYRAKRTTNR AAVMIGAAWG ISLLLWPPWI 250 260 270 280 290 300 YSWPYIEGKR TVPKDECYIQ FIETNQYITF GTALAAFYFP VTIMCFLYWR IWRETKKRQK 310 320 330 340 350 360 DLPNLQAGKK DSSKRSNSSD ENTVVNHASG GLLAFAQVGG NDHDTWRRPR SESSPDAESV 370 380 390 400 410 420 YMTNMVIDSG YHGMHSRKSS IKSTNTIKKS YTCFGSIKEW CIAWWHSGRE DSDDFAYEQE 430 440 450 460 470 480 EPSDLGCTSM NVMRDNYSMG GSVSGVRPPS ILLSDVSPTP LPRPPLASIS QLQEMSAVTA 490 500 510 520 530 540 STTANVNTSG NGNGAINNNN NASHNGNGAV NGNGAGNGSG IGLGTTGNAT HRDSRTLPVI 550 560 570 580 590 600 NRINSRSVSQ DSVYTILIRL PSDGASSNAA NGGGGGPGAG AAASASLSMQ GDCAPSIKMI 610 620 630 640 650 660 HEDGPTTTAA AAPLASAAAT RRPLPSRDSE FSLPLGRRMS HAQHDARLLN AKVIPKQLGK 670 680 690 700 710 720 AGGGAAGGGV GGAHALMNAR NAAKKKKKSQ EKRQESKAAK TLSAILLSFI ITWTPYNILV 730 740 750 760 770 780 LIKPLTTCSD CIPTELWDFF YALCYINSTI NPMCYALCNA TFRRTYVRIL TCKWHTRNRE GMVRGVYN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, sequence analysis and chromosome localization of a Drosophila muscarinic acetylcholine receptor." Onai T., Fitzgerald M.G., Arakawa S., Gocayne J.D., Urquhart D.A., Hall L.M., Fraser C.M., McCombie W.R., Venter J.C. FEBS Lett. 255:219-225(1989) [PubMed: 2507354] [Abstract] Cited for: NUCLEOTIDE SEQUENCE (ISOFORM A).
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
[4]	"Characterization and functional expression in mammalian cells of genomic and cDNA clones encoding a Drosophila muscarinic acetylcholine receptor." Shapiro R.A., Wakimoto B.T., Subers E.M., Nathanson N.M. Proc. Natl. Acad. Sci. U.S.A. 86:9039-9043(1989) [PubMed: 2510174] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 45-805 (ISOFORM B), FUNCTION. Strain: Oregon-R.
[5]	"Localization in the nervous system of Drosophila melanogaster of a C-terminus anti-peptide antibody to a cloned Drosophila muscarinic acetylcholine receptor." Harrison J.B., Chen H.H., Blake A.D., Huskisson N.S., Barker P., Sattelle D.B. J. Neuroendocrinol. 7:347-352(1995) [PubMed: 7550280] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M23412 mRNA. Translation: AAA28676.1. AE013599 Genomic DNA. Translation: AAF47197.1. AE013599 Genomic DNA. Translation: AAM68310.1. M27495 mRNA. Translation: AAA85449.1. Frameshift.
PIR	S05661.
RefSeq	NP_523844.2. NP_726440.1.
UniGene	Dm.2775
3D structure databases
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
Genome annotation databases
Ensembl	CG4356. Drosophila melanogaster. [Contig view]
GeneID	37892.
KEGG	dme:Dmel_CG4356.
NMPDR	fig|7227.3.peg.7297.
Organism-specific databases
FlyBase	FBgn0000037. mAcR-60C.
Phylogenomic databases
HOGENOM	P16395.
Gene expression databases
ArrayExpress	P16395.
GermOnline	CG4356. Drosophila melanogaster.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_supfam. IPR000995. Musac_rcpt. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR00243. MUSCARINICR.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProDom	P16395. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

ACM1_DROME

Accession

Primary (citable) accession number: P16395
Secondary accession number(s): Q8MLP2, Q9W180

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	November 23, 2004
Last modified:	July 22, 2008
This is version 76 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project