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P16497

- KINA_BACSU

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Protein
Sporulation kinase A
Gene
kinA, gsiC, scoD, spoIIF, spoIIJ, BSU13990

Gene:

kinA, gsiC, scoD, spoIIF, spoIIJ, BSU13990
Organism
Bacillus subtilis (strain 168)
Status
Reviewed - - Experimental evidence at protein leveli

UniProt

P16497 - KINA_BACSU

Protein:

Sporulation kinase A
Protein Existence: Experimental evidence at protein level

Functioni

Phosphorylates the sporulation-regulatory proteins spo0A and spo0F. It also autophosphorylates in the presence of ATP.

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

GO - Molecular functioni

  1. ATP bindingInferred from electronic annotationi Source: UniProtKB-KW
  2. identical protein bindingInferred from physical interactioni PubMed 19040634PubMed 19561131 Source: IntAct
  3. phosphorelay sensor kinase activityInferred from electronic annotationi Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. ATP bindingInferred from electronic annotationi Source: UniProtKB-KW
  2. identical protein bindingInferred from physical interactioni PubMed 19040634PubMed 19561131 Source: IntAct
  3. phosphorelay sensor kinase activityInferred from electronic annotationi Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Sporulation, Two-component regulatory system

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU13990-MONOMER.
BRENDAi2.7.13.3. 700.

Names & Taxonomyi

Protein namesi
Recommended name:
Sporulation kinase A (EC:2.7.13.3)
Alternative name(s):
Stage II sporulation protein F
Stage II sporulation protein J
Gene namesi
Name:kinA
Synonyms:gsiC, scoD, spoIIF, spoIIJ
Ordered Locus Names:BSU13990
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570: Chromosome

Organism-specific databases

GenoListiBSU13990. [Micado]

Subcellular locationi

GO - Cellular componenti

  1. membraneInferred from electronic annotationi Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier
Chaini1 – 606606Sporulation kinase A
PRO_0000074776

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier
Modified residuei4051Phosphohistidine; by autocatalysis By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP16497.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
itself7EBI-6405707,EBI-6405707
sdaQ7WY626EBI-6405707,EBI-6405714
spo0FP066282EBI-6405707,EBI-6418009

Protein-protein interaction databases

DIPiDIP-58966N.
IntActiP16497. 2 interactions.
STRINGi224308.BSU13990.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier
Beta strandi17 – 215
Beta strandi25 – 306
Helixi34 – 385
Helixi42 – 454
Helixi50 – 534
Helixi56 – 583
Helixi59 – 679
Beta strandi75 – 806
Beta strandi86 – 9510
Beta strandi107 – 1137

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2VLGX-ray1.70A/B/C/D10-117[»]
ProteinModelPortaliP16497.
SMRiP16497. Positions 15-116, 374-606.

Miscellaneous databases

EvolutionaryTraceiP16497.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier
Domaini3 – 7371PAS 1
Domaini77 – 11640PAC 1
Domaini140 – 21475PAS 2
Domaini218 – 25538PAC 2
Domaini265 – 33571PAS 3
Domaini402 – 606205Histidine kinase

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0642.
HOGENOMiHOG000008904.
KOiK02491.
OMAiRIIYISA.
OrthoDBiEOG69GZGV.
ProtClustDBiCLSK887211.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR003661. EnvZ-like_dim/P.
IPR003594. HATPase_ATP-bd.
IPR001610. PAC.
IPR000014. PAS.
IPR013767. PAS_fold.
IPR004358. Sig_transdc_His_kin-like_C.
IPR005467. Sig_transdc_His_kinase_core.
IPR009082. Sig_transdc_His_kinase_dimeric.
[Graphical view]
PfamiPF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF00989. PAS. 2 hits.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00086. PAC. 2 hits.
SM00091. PAS. 3 hits.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF55785. SSF55785. 3 hits.
SSF55874. SSF55874. 1 hit.
TIGRFAMsiTIGR00229. sensory_box. 3 hits.
PROSITEiPS50109. HIS_KIN. 1 hit.
PS50112. PAS. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P16497-1 [UniParc]FASTA

« Hide

MEQDTQHVKP LQTKTDIHAV LASNGRIIYI SANSKLHLGY LQGEMIGSFL    50
KTFLHEEDQF LVESYFYNEH HLMPCTFRFI KKDHTIVWVE AAVEIVTTRA 100
ERTEREIILK MKVLEEETGH QSLNCEKHEI EPASPESTTY ITDDYERLVE 150
NLPSPLCISV KGKIVYVNSA MLSMLGAKSK DAIIGKSSYE FIEEEYHDIV 200
KNRIIRMQKG MEVGMIEQTW KRLDGTPVHL EVKASPTVYK NQQAELLLLI 250
DISSRKKFQT ILQKSRERYQ LLIQNSIDTI AVIHNGKWVF MNESGISLFE 300
AATYEDLIGK NIYDQLHPCD HEDVKERIQN IAEQKTESEI VKQSWFTFQN 350
RVIYTEMVCI PTTFFGEAAV QVILRDISER KQTEELMLKS EKLSIAGQLA 400
AGIAHEIRNP LTAIKGFLQL MKPTMEGNEH YFDIVFSELS RIELILSELL 450
MLAKPQQNAV KEYLNLKKLI GEVSALLETQ ANLNGIFIRT SYEKDSIYIN 500
GDQNQLKQVF INLIKNAVES MPDGGTVDII ITEDEHSVHV TVKDEGEGIP 550
EKVLNRIGEP FLTTKEKGTG LGLMVTFNII ENHQGVIHVD SHPEKGTAFK 600
ISFPKK 606
Length:606
Mass (Da):69,171
Last modified:July 15, 1998 - v2
Checksum:iA8BB97FE844B32EE
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier
Sequence conflicti2761S → F in AAA22800. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M29450 Genomic DNA. Translation: AAA22800.1.
M31067 Genomic DNA. Translation: AAA22664.1.
AJ222587 Genomic DNA. Translation: CAA10862.1.
AL009126 Genomic DNA. Translation: CAB13272.1.
PIRiA33496.
RefSeqiNP_389282.1. NC_000964.3.

Genome annotation databases

EnsemblBacteriaiCAB13272; CAB13272; BSU13990.
GeneIDi939230.
KEGGibsu:BSU13990.
PATRICi18974581. VBIBacSub10457_1482.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M29450 Genomic DNA. Translation: AAA22800.1 .
M31067 Genomic DNA. Translation: AAA22664.1 .
AJ222587 Genomic DNA. Translation: CAA10862.1 .
AL009126 Genomic DNA. Translation: CAB13272.1 .
PIRi A33496.
RefSeqi NP_389282.1. NC_000964.3.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2VLG X-ray 1.70 A/B/C/D 10-117 [» ]
ProteinModelPortali P16497.
SMRi P16497. Positions 15-116, 374-606.
ModBasei Search...

Protein-protein interaction databases

DIPi DIP-58966N.
IntActi P16497. 2 interactions.
STRINGi 224308.BSU13990.

Chemistry

BindingDBi P16497.
ChEMBLi CHEMBL2111331.

Proteomic databases

PaxDbi P16497.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB13272 ; CAB13272 ; BSU13990 .
GeneIDi 939230.
KEGGi bsu:BSU13990.
PATRICi 18974581. VBIBacSub10457_1482.

Organism-specific databases

GenoListi BSU13990. [Micado ]

Phylogenomic databases

eggNOGi COG0642.
HOGENOMi HOG000008904.
KOi K02491.
OMAi RIIYISA.
OrthoDBi EOG69GZGV.
ProtClustDBi CLSK887211.

Enzyme and pathway databases

BioCyci BSUB:BSU13990-MONOMER.
BRENDAi 2.7.13.3. 700.

Miscellaneous databases

EvolutionaryTracei P16497.

Family and domain databases

Gene3Di 1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProi IPR003661. EnvZ-like_dim/P.
IPR003594. HATPase_ATP-bd.
IPR001610. PAC.
IPR000014. PAS.
IPR013767. PAS_fold.
IPR004358. Sig_transdc_His_kin-like_C.
IPR005467. Sig_transdc_His_kinase_core.
IPR009082. Sig_transdc_His_kinase_dimeric.
[Graphical view ]
Pfami PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF00989. PAS. 2 hits.
[Graphical view ]
PRINTSi PR00344. BCTRLSENSOR.
SMARTi SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00086. PAC. 2 hits.
SM00091. PAS. 3 hits.
[Graphical view ]
SUPFAMi SSF47384. SSF47384. 1 hit.
SSF55785. SSF55785. 3 hits.
SSF55874. SSF55874. 1 hit.
TIGRFAMsi TIGR00229. sensory_box. 3 hits.
PROSITEi PS50109. HIS_KIN. 1 hit.
PS50112. PAS. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publicationsDownload
  1. "The spoIIJ gene, which regulates early developmental steps in Bacillus subtilis, belongs to a class of environmentally responsive genes."
    Antoniewski C., Savelli B., Stragier P.
    J. Bacteriol. 172:86-93(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Characterization of the gene for a protein kinase which phosphorylates the sporulation-regulatory proteins Spo0A and Spo0F of Bacillus subtilis."
    Perego M., Cole S.P., Burbulys D., Trach K., Hoch J.A.
    J. Bacteriol. 171:6187-6196(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  3. "Sequence of the Bacillus subtilis chromosome from ykuA to cse-15."
    Scanlan E., Devine K.M.
    Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  4. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.

Entry nameiKINA_BACSU
AccessioniPrimary (citable) accession number: P16497
Secondary accession number(s): P22863
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: July 15, 1998
Last modified: April 16, 2014
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

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