Reviewed, UniProtKB/Swiss-Prot P17044 (MTBF_BACSU)

Names and origin

Protein names

Recommended name:
    Modification methylase BsuFI
      Short name(s)=M.BsuFI
    EC=2.1.1.37
Alternative name(s):
    Cytosine-specific methyltransferase BsuFI

Gene names

Name:	hsdFM
Synonyms:	hsmF

Organism

Bacillus subtilis

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	409 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence CCGG, causes specific methylation on C-1 on both strands, and protects the DNA from cleavage by the BsuFI endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
Sequence similarities	Belongs to the C5-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Molecular function	DNA (cytosine-5-)-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 409	409	Modification methylase BsuFI
Sites
	Active site	170	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P17044-1 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: 9CBE3732596A3C20	FASTA	409	46,911
	10 20 30 40 50 60 MRGGNRLGAG RKVIPESEKK KRKSVYITDK LYTRIMDTDI ENCNNFSQKC MALIELAMEN 70 80 90 100 110 120 LNKNNQEHSV KRNNILMVRD TKSTYNKTNN NFEKQNRGIK LTFIDLFAGI GGIRLGFEDK 130 140 150 160 170 180 YTKCVFSSEW DKYAAQTYEA NYGEKPHGDI TKINENDIPD QDVLLAGFPC QPFSNIGKRE 190 200 210 220 230 240 GFAHERRNII FDVLRILKKK QPKMFLLENV KGLLTNDNGN TFRVILDNLK SLGYSVFYEV 250 260 270 280 290 300 MDAQNFGLPQ RRERIVIVGF HPDLGINDFS FPKGNPDNKV PINAILEHNP TGYSISKRLQ 310 320 330 340 350 360 ESYLFKKDDG KPQIVDFRCT YQVNTLVASY HKIQRLTGTF VKDGETGLRL FSELELKRLM 370 380 390 400 GFPVDFKVPV SRTQMYRQFG NSVAVPMIKA VAGAMKERLL LAEMQVLKK

References

[1]

"The amino acid sequence of the CCGG recognizing DNA methyltransferase M.BsuFI: implications for the analysis of sequence recognition by cytosine DNA methyltransferases." Walter J., Noyer-Weidner M., Trautner T.A. EMBO J. 9:1007-1013(1990) [PubMed: 2108858] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ISF18.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X51515 Genomic DNA. Translation: CAA35888.1.
PIR	CTBSFI. S09247.
3D structure databases
HSSP	HSSP built from PDB template 6MHT based on UniProtKB P05102.
ModBase	Search...
Protein family/group databases
REBASE	3338. M.BsuFI.
Family and domain databases
InterPro	IPR001525. C5_DNA_meth. [Graphical view]
PANTHER	PTHR10629. C5_DNA_meth. 1 hit.
Pfam	PF00145. DNA_methylase. 1 hit. [Graphical view]
PRINTS	PR00105. C5METTRFRASE.
TIGRFAMs	TIGR00675. dcm. 1 hit.
PROSITE	PS00094. C5_MTASE_1. 1 hit. PS00095. C5_MTASE_2. 1 hit. [Graphical view]
ProDom	P17044. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

MTBF_BACSU

Accession

Primary (citable) accession number: P17044

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	July 22, 2008
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)