Reviewed, UniProtKB/Swiss-Prot P17676 (CEBPB_HUMAN)

Names and origin

Protein names

Recommended name:
    CCAAT/enhancer-binding protein beta
      Short name=C/EBP beta
Alternative name(s):
    Nuclear factor NF-IL6
    Transcription factor 5

Gene names

Name:	CEBPB
Synonyms:	TCF5
ORF Names:	PP9092

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Important transcriptional activator in the regulation of genes involved in immune and inflammatory responses. Specifically binds to an IL-1 response element in the IL-6 gene. NF-IL6 also binds to regulatory regions of several acute-phase and cytokines genes. It probably plays a role in the regulation of acute-phase reaction, inflammation and hemopoiesis. The consensus recognition site is 5'-T[TG]NNGNAA[TG]-3'.
Subunit structure	Binds DNA as a dimer and can form stable heterodimers with C/EBP alpha, delta and gamma. Interacts with TRIM28 and PTGES2 By similarity.
Subcellular location	Nucleus.
Tissue specificity	Expressed at low levels in the lung, kidney and spleen.
Post-translational modification	Sumoylated by polymeric chains of SUMO2 or SUMO3.
Sequence similarities	Belongs to the bZIP family. C/EBP subfamily. Contains 1 bZIP domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Ligand	DNA-binding
Molecular function	Activator
PTM	Ubl conjugation
Technical term	3D-structure
Gene Ontology (GO)
Biological process	acute-phase response Ref.1 Traceable author statement. Source: ProtInc immune response Ref.1 Traceable author statement. Source: ProtInc transcription from RNA polymerase II promoter Traceable author statement. Source: ProtInc
Cellular component	nucleus Traceable author statement. Source: ProtInc
Molecular function	protein binding Inferred from physical interaction. Source: IntAct transcription factor activity Non-traceable author statement. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SMAD4	Q13485	1	EBI-969696,EBI-347263
TP53	P04637	3	EBI-969696,EBI-366083

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Molecule processing

Chain

1 – 345

345

CCAAT/enhancer-binding protein beta

Regions

Domain

306 – 334

29

Leucine-zipper

DNA binding

277 – 293

17

Basic motif

Region

1 – 24

24

Required for Lys-174 sumoylation

Compositional bias

162 – 170

9

Poly-Pro

Amino acid modifications

Cross-link

174

Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)

Natural variations

Natural variant

195

1

G → S: dbSNP rs4253440.

Experimental info

Sequence conflict

241

1

A → P Ref.6

Sequence conflict

253

1

A → G in CAA36794. Ref.1

Secondary structure

1

.

.

.

.

345

Helix Strand Turn

Details...

Helix

272 – 328

57

Turn

329 – 331

3

Sequences

	Sequence		Length	Mass (Da)
	P17676-1 [UniParc]. Last modified August 22, 2003. Version 2. Checksum: 9B725BD6CCAA771D	FASTA	345	36,106
	10 20 30 40 50 60 MQRLVAWDPA CLPLPPPPPA FKSMEVANFY YEADCLAAAY GGKAAPAAPP AARPGPRPPA 70 80 90 100 110 120 GELGSIGDHE RAIDFSPYLE PLGAPQAPAP ATATDTFEAA PPAPAPAPAS SGQHHDFLSD 130 140 150 160 170 180 LFSDDYGGKN CKKPAEYGYV SLGRLGAAKG ALHPGCFAPL HPPPPPPPPP AELKAEPGFE 190 200 210 220 230 240 PADCKRKEEA GAPGGGAGMA AGFPYALRAY LGYQAVPSGS SGSLSTSSSS SPPGTPSPAD 250 260 270 280 290 300 AKAPPTACYA GAAPAPSQVK SKAKKTVDKH SDEYKIRRER NNIAVRKSRD KAKMRNLETQ 310 320 330 340 HKVLELTAEN ERLQKKVEQL SRELSTLRNL FKQLPEPLLA SSGHC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A nuclear factor for IL-6 expression (NF-IL6) is a member of a C/EBP family." Akira S., Isshiki H., Sugita T., Tanabe O., Kinoshita S., Nishio Y., Nakajima T., Hirano T., Kishimoto T. EMBO J. 9:1897-1906(1990) [PubMed: 2112087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Placenta.
[2]	"Large-scale cDNA transfection screening for genes related to cancer development and progression." Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H., Qiu X., Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y., Shu H., Chen X. , Xu H., Guo M., Pan Z., Chen Y., Ge C., Yang S., Gu J. Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004) [PubMed: 15498874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	SeattleSNPs program for genomic applications Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT SER-195.
[4]	"The DNA sequence and comparative analysis of human chromosome 20." Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J. Nature 414:865-871(2001) [PubMed: 11780052] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Muscle and Skin.
[6]	"Identification of a transcriptional regulatory factor for human aromatase cytochrome P450 gene expression as nuclear factor interleukin-6 (NF-IL6), a member of the CCAAT/enhancer-binding protein family." Toda K., Akira S., Kishimoto T., Sasaki H., Hashimoto K., Yamamoto Y., Sagara Y., Shizuta Y. Eur. J. Biochem. 231:292-299(1995) [PubMed: 7635140] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 241-323.
[7]	"Modification of CCAAT/enhancer-binding protein-beta by the small ubiquitin-like modifier (SUMO) family members, SUMO-2 and SUMO-3." Eaton E.M., Sealy L. J. Biol. Chem. 278:33416-33421(2003) [PubMed: 12810706] [Abstract] Cited for: SUMOYLATION AT LYS-174.
[8]	"Structural analyses of DNA recognition by the AML1/Runx-1 Runt domain and its allosteric control by CBFbeta." Tahirov T.H., Inoue-Bungo T., Morii H., Fujikawa A., Sasaki M., Kimura K., Shiina M., Sato K., Kumasaka T., Yamamoto M., Ishii S., Ogata K. Cell 104:755-767(2001) [PubMed: 11257229] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.65 ANGSTROMS) OF 259-336 IN COMPLEX WITH RUNX1; CBFB AND DNA.
[9]	"Mechanism of c-Myb-C/EBP beta cooperation from separated sites on a promoter." Tahirov T.H., Sato K., Ichikawa-Iwata E., Sasaki M., Inoue-Bungo T., Shiina M., Kimura K., Takata S., Fujikawa A., Morii H., Kumasaka T., Yamamoto M., Ishii S., Ogata K. Cell 108:57-70(2002) [PubMed: 11792321] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 259-345 IN COMPLEX WITH MOUSE MYB.
+	Additional computationally mapped references.

Web resources

SeattleSNPs

Cross-references

Sequence databases

EMBLGenBankDDBJ

X52560 Genomic DNA. Translation: CAA36794.1.
AF289608 mRNA. Translation: AAL55792.1.
AY193834 Genomic DNA. Translation: AAN86350.1.
AL161937 Genomic DNA. Translation: CAC14276.1.
BC007538 mRNA. Translation: AAH07538.1.
BC021931 mRNA. Translation: AAH21931.1.

PIR

S12788.
S66246.

RefSeq

NP_005185.2.

UniGene

Hs.517106
Hs.688479

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1GTW	X-ray	1.85	A/B	259-336	[»]
1GU4	X-ray	1.80	A/B	259-336	[»]
1GU5	X-ray	2.10	A/B	259-336	[»]
1H88	X-ray	2.80	A/B	259-336	[»]
1H89	X-ray	2.45	A/B	273-336	[»]
1H8A	X-ray	2.23	A/B	259-336	[»]
1HJB	X-ray	3.00	A/B/D/E	259-345	[»]
1IO4	X-ray	3.00	A/B	259-336	[»]
2E42	X-ray	1.80	A/B	259-336	[»]
2E43	X-ray	2.10	A/B	259-336	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P17676.

PTM databases

PhosphoSite

P17676.

Proteomic databases

PeptideAtlas

P17676.

Genome annotation databases

Ensembl

ENSG00000172216. Homo sapiens. [Contig view]

GeneID

1051.

KEGG

hsa:1051.

Organism-specific databases

H-InvDB

HIX0015909.

HGNC

HGNC:1834. CEBPB.

HPA

CAB004213.

MIM

189965. gene.

PharmGKB

PA26377.

GenAtlas

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GeneCards

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Phylogenomic databases

HOGENOM

P17676.

HOVERGEN

P17676.

Gene expression databases

ArrayExpress

P17676.

CleanEx

HS_CEBPB.

GermOnline

ENSG00000172216. Homo sapiens.

Family and domain databases

InterPro

IPR011700. bZIP_2.
IPR016468. CCAAT/enhancer-binding.
IPR004827. TF_bZIP.
[Graphical view]

Pfam

PF07716. bZIP_2. 1 hit.
[Graphical view]

PIRSF

PIRSF005879. CCAAT/enhancer-binding. 1 hit.

SMART

SM00338. BRLZ. 1 hit.
[Graphical view]

PROSITE

PS50217. BZIP. 1 hit.
PS00036. BZIP_BASIC. False negative.
[Graphical view]

ProDom

P17676.
[Graphical view] [Entries sharing at least one domain]

BLOCKS

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Other Resources

LinkHub

P17676.

SOURCE

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ProtoNet

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Entry information

Entry name

CEBPB_HUMAN

Accession

Primary (citable) accession number: P17676
Secondary accession number(s): Q96IH2, Q9H4Z5

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 22, 2003
Last modified:	July 22, 2008
This is version 100 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)