Reviewed, UniProtKB/Swiss-Prot P17814 (4CL1_ORYSJ)

Names and origin

Protein names

Recommended name:
    4-coumarate--CoA ligase 1
      Short name=4CL 1
    EC=6.2.1.12
Alternative name(s):
    4-coumaroyl-CoA synthase 1

Gene names

Name:	4CL1
Synonyms:	4CL
Ordered Locus Names:	Os08g0245200, LOC_Os08g14760
ORF Names:	OJ1033_B09.16

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	564 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + 4-coumarate + CoA = AMP + diphosphate + 4-coumaroyl-CoA.
Pathway	Phytoalexin biosynthesis; 3,4',5-trihydroxystilbene biosynthesis; 3,4',5-trihydroxystilbene from 4-coumaric acid: step 1/2.
Induction	By fungal elicitor and UV irradiation.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.
Sequence caution	The sequence CAA36850.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAA36850.1 differs from that shown. Reason: Frameshift at position 496.

Ontologies

Keywords
Biological process	Phenylpropanoid metabolism
Molecular function	Ligase
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 564	564	4-coumarate--CoA ligase 1
Experimental info
	Sequence conflict	405	1	P → R in CAA36850. Ref.1
	Sequence conflict	477	1	I → N in CAA36850. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P17814-1 [UniParc]. Last modified September 27, 2005. Version 2. Checksum: 9725A4B4A72F8C6E	FASTA	564	60,902
	10 20 30 40 50 60 MGSMEQQQPE SAAPATEASP EIIFRSKLQD IAITNTLPLH RYCFERLPEV AARPCLIDGA 70 80 90 100 110 120 TGGVLTYADV DRLSRRLAAA LRRAPLGLRR GGVVMSLLRN SPEFVLSFFA ASRVGAAVTT 130 140 150 160 170 180 ANPMSTPHEI ESQLAAAGAT VVITESMAAD KLPSHSHGAL TVVLIDERRD GCLHFWDDLM 190 200 210 220 230 240 SEDEASPLAG DEDDEKVFDP DDVVALPYSS GTTGLPKGVM LTHRSLSTSV AQQVDGENPN 250 260 270 280 290 300 IGLHAGDVIL CALPMFHIYS LNTIMMCGLR VGAAIVVMRR FDLAAMMDLV ERHRVTIAPL 310 320 330 340 350 360 VPPIVVAVAK SEAAAARDLS SVRMVLSGAA PMGKDIEDAF MAKLPGAVLG QGYGMTEAGP 370 380 390 400 410 420 VLSMCLAFAK EPFKVKSGAC GTVVRNAELK IIDPDTGKSL GRNLPGEICI RGQQIMKGYL 430 440 450 460 470 480 NNPEATKNTI DAEGWLHTGD IGYVDDDDEI FIVDRLKEII KYRGFQVAPA ELEALLITHP 490 500 510 520 530 540 SIADAAVVGK QIEPEIGEIP VAFVAKTEGS ELSEDDVKQF VAKEVIYYKK IREVFFVDKI 550 560 PKAPSGKILR KELRKQLQHL QQEA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of rice 4-coumarate:CoA ligase gene, 4-CL.1." Zhao Y., Kung S.D., Dube S.K. Nucleic Acids Res. 18:6144-6144(1990) [PubMed: 2235510] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Oryza sativa nipponbare chromosome 8 genomic DNA sequence." The international rice genome sequencing project (IRGSP) consortium Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X52623 Genomic DNA. Translation: CAA36850.1. Sequence problems. AP003859 Genomic DNA. Translation: BAD05189.1. Different initiation. AK069932 mRNA. No translation available.
PIR	JU0311.
RefSeq	NP_001061353.1.
UniGene	Os.52921
3D structure databases
HSSP	HSSP built from PDB template 1LCI based on UniProtKB P08659.
ModBase	Search...
Genome annotation databases
GeneID	4345054.
KEGG	osa:4345054.
Organism-specific databases
Gramene	P17814.
Family and domain databases
InterPro	IPR000873. AMP-dep_Synth/Lig. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
PRINTS	PR00154. AMPBINDING.
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProDom	P17814. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

4CL1_ORYSJ

Accession

Primary (citable) accession number: P17814
Secondary accession number(s): Q6ZKV9

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	September 27, 2005
Last modified:	September 2, 2008
This is version 58 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)