Reviewed, UniProtKB/Swiss-Prot P18051 (MTB2_BACAM)

Names and origin

Protein names

Recommended name:
    Modification methylase BamHII
      Short name=M.BamHII
    EC=2.1.1.113
Alternative name(s):
    N(4)-cytosine-specific methyltransferase BamHII

Gene names

Name:

bamHIIM

Organism

Bacillus amyloliquefaciens

Taxonomic identifier

1390 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	265 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence GGATCC, causes specific methylation on C-5 on both strands, and protects the DNA from cleavage by the BamHI endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA cytosine = S-adenosyl-L-homocysteine + DNA N(4)-methylcytosine.
Miscellaneous	This methylase is encoded on the proviral phage H2.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 265	265	Modification methylase BamHII

Sequences

	Sequence		Length	Mass (Da)
	P18051-1 [UniParc]. Last modified November 1, 1990. Version 1. Checksum: 895A51217A814820	FASTA	265	30,981
	10 20 30 40 50 60 MKENIGDCTI DLTVTSPPYD DLRNYNGYSF NFEETAQELY RVTKEGGVVV WVVGDKTHKG 70 80 90 100 110 120 SETGSSFRQA LYFKELGFNL HDTMIYEKDS ISFPDKNRYY QIFEYMFIFS KGKPKTINLL 130 140 150 160 170 180 ADRKNKWYNG KKHIKGHYRK MDGEKVRHHK QNLLKEFGVR FNIWRIPNGH QKSTLDKIAF 190 200 210 220 230 240 QHPAIFPEKL AEDHILSWSN EGDIVFDPFM GSGTTAKMAA LNNRKYIGTE ISKEYCDIAN 250 260 ERLKNYIILH KRMEGKGYRL PQVHS

References

[1]	"The complete sequence of the Bacillus amyloliquefaciens proviral H2, BamHI methylase gene." Connaughton J.F., Kaloss W.D., Vanek P.G., Nardone G.A., Chirikjian J.G. Nucleic Acids Res. 18:4002-4002(1990) [PubMed: 2374727] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: H.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X53032 Genomic DNA. Translation: CAA37205.1.
PIR	S10316.
3D structure databases
HSSP	HSSP built from PDB template 1G60 based on UniProtKB P23192.
ModBase	Search...
Protein family/group databases
REBASE	186. M.BamHII.
Family and domain databases
InterPro	IPR002295. D21N6_MeTrfase. IPR001091. MeTrfase_CN4. IPR002941. N6/N4_Mtase. [Graphical view]
Pfam	PF01555. N6_N4_Mtase. 1 hit. [Graphical view]
PRINTS	PR00506. D21N6MTFRASE. PR00508. S21N4MTFRASE.
PROSITE	PS00093. N4_MTASE. 1 hit. [Graphical view]
ProDom	P18051. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

MTB2_BACAM

Accession

Primary (citable) accession number: P18051

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	November 1, 1990
Last modified:	September 2, 2008
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)