Reviewed, UniProtKB/Swiss-Prot P18276 (CHYM_SHEEP)

Names and origin

Protein names

Recommended name:
    Chymosin
    EC=3.4.23.4
Alternative name(s):
    Preprorennin

Gene names

Name:

CYM

Organism

Ovis aries (Sheep)

Taxonomic identifier

9940 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Caprinae › Ovis

Protein attributes

Sequence length	381 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Chymosin is synthesized in the mucosa of the stomach. The enzyme hydrolyzes casein to paracasein.
Catalytic activity	Broad specificity similar to that of pepsin A. Clots milk by cleavage of a single 105-Ser-Phe-|-Met-Ala-108 bond in kappa-chain of casein.
Subunit structure	Monomer.
Sequence similarities	Belongs to the peptidase A1 family.

Ontologies

Keywords
Biological process	Digestion
Domain	Signal
Molecular function	Aspartyl protease Hydrolase Protease
PTM	Zymogen
Gene Ontology (GO)
Molecular function	chymosin activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Signal peptide	1 – 16	16
	Propeptide	17 – 58	42	Activation peptide
	Chain	59 – 381	323	Chymosin
Sites
	Active site	92	1	By similarity
	Active site	274	1	By similarity
Amino acid modifications
	Disulfide bond	105 ↔ 110		By similarity
	Disulfide bond	265 ↔ 269		By similarity
	Disulfide bond	308 ↔ 341		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P18276-1 [UniParc]. Last modified November 1, 1990. Version 1. Checksum: D9903528FA071C47	FASTA	381	42,075
	10 20 30 40 50 60 MRCLVVLLAV FALSQGAEIT RIPLYKGKPL RKALKERGLL EDFLQKQQYG VSSEYSGFGE 70 80 90 100 110 120 VASVPLTNYL DSQYFGKIYL GTPPQEFTVL FDTGSSDFWV PSIYCKSNAC KNHQRFDPRK 130 140 150 160 170 180 SSTFQNLGKP LSIRYGTGSM QGILGYDTVT VSNIVDIQQT VGLSTQEPGD VFTYAEFDGI 190 200 210 220 230 240 LGMAYPSLAS EYSVPVFDNM MDRRLVAQDL FSVYMDRSGQ GSMLTLGAID PSYYTGSLHW 250 260 270 280 290 300 VPVTLQKYWQ FTVDSVTISG AVVACEGGCQ AILDTGTSKL VGPSSDILNI QQAIGATQNQ 310 320 330 340 350 360 YGEFDIDCDS LSSMPTVVFE INGKMYPLTP YAYTSQEEGF CTSGFQGENH SHQWILGDVF 370 380 IREYYSVFDR ANNLVGLAKA I

References

[1]	"Complete primary structure of lamb preprochymosin deduced from cDNA." Pungecar J., Strukelj B., Gubensek F., Turk V., Kregar I. Nucleic Acids Res. 18:4602-4602(1990) [PubMed: 2117748] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X53037 mRNA. Translation: CAA37209.1.
PIR	CMSHB. S10996.
RefSeq	NP_001009804.1.
UniGene	Oar.447
3D structure databases
HSSP	HSSP built from PDB template 1CMS based on UniProtKB P00794.
SMR	P18276. Positions 59-381.
ModBase	Search...
Protein family/group databases
MEROPS	A01.006.
Genome annotation databases
GeneID	443399.
Phylogenomic databases
HOVERGEN	P18276.
Family and domain databases
InterPro	IPR001969. Pept_Asp_AS. IPR009007. Pept_Aspartc_cat. IPR001461. Peptidase_A1. IPR012848. Propep_A1. [Graphical view]
Gene3D	G3DSA:2.40.70.10. Pept_Aspartc_cat. 1 hit.
PANTHER	PTHR13683. Peptidase_A1. 1 hit.
Pfam	PF07966. A1_Propeptide. 1 hit. PF00026. Asp. 1 hit. [Graphical view]
PRINTS	PR00792. PEPSIN.
PROSITE	PS00141. ASP_PROTEASE. 2 hits. [Graphical view]
ProDom	P18276. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

CHYM_SHEEP

Accession

Primary (citable) accession number: P18276

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	November 1, 1990
Last modified:	July 22, 2008
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)