Lymphocyte function-associated antigen 3 precursor

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Reviewed, UniProtKB/Swiss-Prot P19256 (LFA3_HUMAN)

Last modified July 22, 2008. Version 93. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Lymphocyte function-associated antigen 3
      Short name=Ag3
Alternative name(s):
    Surface glycoprotein LFA-3
    CD_antigen=CD58

Gene names

Name:	CD58
Synonyms:	LFA3

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	250 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function

Ligand of the T-lymphocyte CD2 glycoprotein. This interaction is important in mediating thymocyte interactions with thymic epithelial cells, antigen-independent and -dependent interactions of T-lymphocytes with target cells and antigen-presenting cells and the T-lymphocyte rosetting with erythrocytes. In addition, the LFA-3/CD2 interaction may prime response by both the CD2+ and LFA-3+ cells.

Subcellular location

Isoform 1: Cell membrane; Single-pass type I membrane protein.

Isoform 2: Cell membrane; Lipid-anchor › GPI-anchor.

Sequence similarities

Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

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Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Domain	Immunoglobulin domain Signal Transmembrane
PTM	GPI-anchor Glycoprotein Lipoprotein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	cell-cell adhesion Non-traceable author statement. Source: UniProtKB
Cellular component	integral to plasma membrane Non-traceable author statement. Source: UniProtKB
Molecular function	protein binding Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

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Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P19256-1) Also known as: Long; This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P19256-2) Also known as: Short; The sequence of this isoform differs from the canonical sequence as follows: 236-237: GI → VL 238-250: Missing.
Notes: GPI-anchored form. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Molecule processing

Signal peptide

1 – 28

Chain

29 – 235

207

Lymphocyte function-associated antigen 3

Propeptide

236 – 250

Removed in mature form Probable

Regions

Topological domain

29 – 215

187

Extracellular Potential

Transmembrane

216 – 238

Potential

Topological domain

239 – 250

Cytoplasmic Potential

Domain

135 – 194

Ig-like C2-type

Amino acid modifications

Lipidation

235

GPI-anchor amidated asparagine Probable

Glycosylation

N-linked (GlcNAc...)

Glycosylation

N-linked (GlcNAc...)

Glycosylation

109

N-linked (GlcNAc...) Potential

Glycosylation

135

N-linked (GlcNAc...) Potential

Glycosylation

169

N-linked (GlcNAc...) Potential

Glycosylation

195

N-linked (GlcNAc...) Potential

Disulfide bond

142 ↔ 187

By similarity

Natural variations

Alternative sequence

236 – 237

GI → VL in isoform 2.

Alternative sequence

238 – 250

Missing in isoform 2.

Secondary structure

250

Helix Strand Turn

Details...

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Sequences

Sequence		Length	Mass (Da)
Isoform 1 (Long) [UniParc]. Last modified November 1, 1990. Version 1. Checksum: 34D635DF1D14FE2E Show »	FASTA	250	28,147
10 20 30 40 50 60 MVAGSDAGRA LGVLSVVCLL HCFGFISCFS QQIYGVVYGN VTFHVPSNVP LKEVLWKKQK 70 80 90 100 110 120 DKVAELENSE FRAFSSFKNR VYLDTVSGSL TIYNLTSSDE DEYEMESPNI TDTMKFFLYV 130 140 150 160 170 180 LESLPSPTLT CALTNGSIEV QCMIPEHYNS HRGLIMYSWD CPMEQCKRNS TSIYFKMEND 190 200 210 220 230 240 LPQKIQCTLS NPLFNTTSSI ILTTCIPSSG HSRHRYALIP IPLAVITTCI VLYMNGILKC 250 DRKPDRTNSN « Hide
Isoform 2 (Short) [UniParc]. Checksum: 9FF77F81A224D8DC Show »		237	26,661
10 20 30 40 50 60 MVAGSDAGRA LGVLSVVCLL HCFGFISCFS QQIYGVVYGN VTFHVPSNVP LKEVLWKKQK 70 80 90 100 110 120 DKVAELENSE FRAFSSFKNR VYLDTVSGSL TIYNLTSSDE DEYEMESPNI TDTMKFFLYV 130 140 150 160 170 180 LESLPSPTLT CALTNGSIEV QCMIPEHYNS HRGLIMYSWD CPMEQCKRNS TSIYFKMEND 190 200 210 220 230 LPQKIQCTLS NPLFNTTSSI ILTTCIPSSG HSRHRYALIP IPLAVITTCI VLYMNVL « Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Primary structure of lymphocyte function-associated antigen 3 (LFA-3). The ligand of the T lymphocyte CD2 glycoprotein." Wallner B.P., Frey A.Z., Tizard R., Mattaliano R.J., Hession C., Sanders M.E., Dustin M.L., Springer T.A. J. Exp. Med. 166:923-932(1987) [PubMed: 3309127] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PARTIAL PROTEIN SEQUENCE. Tissue: Erythrocyte.
[2]	"An LFA-3 cDNA encodes a phospholipid-linked membrane protein homologous to its receptor CD2." Seed B. Nature 329:840-842(1987) [PubMed: 3313052] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[3]	"Gene structure, promoter characterization, and basis for alternative mRNA splicing of the human CD58 gene." Wallich R., Brenner C., Brand Y., Roux M., Reister M., Meuer S. J. Immunol. 160:2862-2871(1998) [PubMed: 9510189] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1 AND 2).
[4]	"An unappreciated role for RNA surveillance." Hillman R.T., Green R.E., Brenner S.E. Genome Biol. 5:RESEARCH008.1-RESEARCH008.16(2004) [PubMed: 14759258] [Abstract] Cited for: SPLICE ISOFORM(S) THAT ARE POTENTIAL NMD TARGET(S).
[5]	"Modification-specific proteomics of plasma membrane proteins: identification and characterization of glycosylphosphatidylinositol-anchored proteins released upon phospholipase D treatment." Elortza F., Mohammed S., Bunkenborg J., Foster L.J., Nuehse T.S., Brodbeck U., Peck S.C., Jensen O.N. J. Proteome Res. 5:935-943(2006) [PubMed: 16602701] [Abstract] Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[6]	"Crystal structure of the CD2-binding domain of CD58 (lymphocyte function-associated antigen 3) at 1.8-A resolution." Ikemizu S., Sparks L.M., van der Merwe P.A., Harlos K., Stuart D.I., Jones E.Y., Davis S.J. Proc. Natl. Acad. Sci. U.S.A. 96:4289-4294(1999) [PubMed: 10200255] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 29-122.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

Y00636 mRNA. Translation: CAA68668.1.
X06296 mRNA. Translation: CAA29622.1.
Y14780

Y14784 Genomic DNA. Translation: CAA75083.1.
Y14780

Y14785 Genomic DNA. Translation: CAA75084.1.

PIR

A28564.

RefSeq

NP_001770.1.

UniGene

Hs.34341

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1CCZ	X-ray	1.80	A	29-190	[»]
1CI5	NMR	-	A	30-123	[»]
1QA9	X-ray	3.20	B/D	30-123	[»]

ModBase

Search...

Genome annotation databases

Ensembl

ENSG00000116815. Homo sapiens. [Contig view]

GeneID

965.

KEGG

hsa:965.

Organism-specific databases

H-InvDB

HIX0000929.

HGNC

HGNC:1688. CD58.

MIM

153420. gene.

PharmGKB

PA26227.

GenAtlas

Search...

GeneCards

Search...

Phylogenomic databases

HOGENOM

P19256.

HOVERGEN

P19256.

Gene expression databases

ArrayExpress

P19256.

CleanEx

HS_CD58.

GermOnline

ENSG00000116815. Homo sapiens.

Family and domain databases

InterPro

IPR007110. Ig-like.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR015714. Lymphocyte_antigen_3.
[Graphical view]

Gene3D

G3DSA:2.60.40.10. Ig-like_fold. 1 hit.

PANTHER

PTHR21063. lfa_3. 1 hit.

SMART

SM00409. IG. 1 hit.
[Graphical view]

PROSITE

PS50835. IG_LIKE. False negative.
[Graphical view]

ProDom

P19256.
[Graphical view] [Entries sharing at least one domain]

BLOCKS

Search...

Other Resources

SOURCE

Search...

ProtoNet

Search...

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Entry information

Entry name

LFA3_HUMAN

Accession

Primary (citable) accession number: P19256
Secondary accession number(s): Q96KI9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	November 1, 1990
Last modified:	July 22, 2008
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

Human cell differentiation molecules

CD nomenclature of surface proteins of human leucocytes and list of entries

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

UniProtKB secondary accession numbers

Index of UniProtKB secondary accession numbers

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Keywords

Gene Ontology (GO)

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Natural variations

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents