Reviewed, UniProtKB/Swiss-Prot P20334 (TNR9_MOUSE)

Names and origin

Protein names

Recommended name:
    Tumor necrosis factor receptor superfamily member 9
Alternative name(s):
    4-1BB ligand receptor
    T-cell antigen 4-1BB
    CD_antigen=CD137

Gene names

Name:	Tnfrsf9
Synonyms:	Cd137, Ila, Ly63

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	256 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Receptor for TNFSF14/4-1BBL. Possibly active during T cell activation.
Subunit structure	Principally an homodimer, but also found as a monomer. Associates with p56-LCK. Interacts with TRAF1, TRAF2 and TRAF3 By similarity.
Subcellular location	Membrane; Single-pass type I membrane protein.
Tissue specificity	Expressed on the surface of activated T-cells.
Induction	Optimal by PMA and ionomycin.
Sequence similarities	Contains 4 TNFR-Cys repeats.

Ontologies

Keywords
Cellular component	Membrane
Domain	Repeat Signal Transmembrane
Molecular function	Receptor
PTM	Glycoprotein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
TRAF2	Q12933	1	EBI-520693,EBI-355744	From a different organism.
Traf2	P39429	2	EBI-520693,EBI-520016

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Molecule processing

Signal peptide

1 – 23

23

Chain

24 – 256

233

Tumor necrosis factor receptor superfamily member 9

Regions

Topological domain

24 – 187

164

Extracellular Potential

Transmembrane

188 – 208

21

Potential

Topological domain

209 – 256

48

Cytoplasmic Potential

Repeat

24 – 45

22

TNFR-Cys 1

Repeat

46 – 85

40

TNFR-Cys 2

Repeat

86 – 117

32

TNFR-Cys 3

Repeat

118 – 159

42

TNFR-Cys 4

Amino acid modifications

Glycosylation

128

1

N-linked (GlcNAc...) Potential

Glycosylation

138

1

N-linked (GlcNAc...) Potential

Disulfide bond

28 ↔ 37

By similarity

Disulfide bond

31 ↔ 44

By similarity

Disulfide bond

47 ↔ 61

By similarity

Disulfide bond

64 ↔ 77

By similarity

Disulfide bond

67 ↔ 85

By similarity

Disulfide bond

87 ↔ 93

By similarity

Disulfide bond

98 ↔ 105

By similarity

Disulfide bond

101 ↔ 116

By similarity

Disulfide bond

119 ↔ 133

By similarity

Disulfide bond

139 ↔ 158

By similarity

Secondary structure

1

.

.

.

256

Helix Strand Turn

Details...

Beta strand

233 – 234

2

Sequences

	Sequence		Length	Mass (Da)
	P20334-1 [UniParc]. Last modified February 1, 1991. Version 1. Checksum: 93A10D03C60813C4	FASTA	256	27,598
	10 20 30 40 50 60 MGNNCYNVVV IVLLLVGCEK VGAVQNSCDN CQPGTFCRKY NPVCKSCPPS TFSSIGGQPN 70 80 90 100 110 120 CNICRVCAGY FRFKKFCSST HNAECECIEG FHCLGPQCTR CEKDCRPGQE LTKQGCKTCS 130 140 150 160 170 180 LGTFNDQNGT GVCRPWTNCS LDGRSVLKTG TTEKDVVCGP PVVSFSPSTT ISVTPEGGPG 190 200 210 220 230 240 GHSLQVLTLF LALTSALLLA LIFITLLFSV LKWIRKKFPH IFKQPFKKTT GAAQEEDACS 250 CRCPQEEEGG GGGYEL

References

[1]	"cDNA sequences of two inducible T-cell genes." Kwon B.S., Weissman S.M. Proc. Natl. Acad. Sci. U.S.A. 86:1963-1967(1989) [PubMed: 2784565] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Genomic organization and chromosomal localization of the T-cell antigen 4-1BB." Kwon B.S., Kozak C.A., Kim K.K., Pickard R.T. J. Immunol. 152:2256-2262(1994) [PubMed: 8133039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: BALB/c.
[3]	"Inducible T cell antigen 4-1BB. Analysis of expression and function." Pollok K.E., Kim Y.-J., Zhou Z., Hurtado J., Kin K.K., Pickard R.T., Kwon B.S. J. Immunol. 150:771-781(1993) [PubMed: 7678621] [Abstract] Cited for: CHARACTERIZATION, PROTEIN SEQUENCE OF 24-29.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

J04492 mRNA. Translation: AAA40167.1.
U02567 Genomic DNA. Translation: AAA93113.1.

PIR

B32393.

RefSeq

NP_001070977.1.
NP_035742.1.

UniGene

Mm.244187

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1D0J	X-ray	2.50	G/H/I/J/K	231-236	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:1154N.

IntAct

P20334.

Genome annotation databases

Ensembl

ENSMUSG00000028965. Mus musculus. [Contig view]

GeneID

21942.

Organism-specific databases

MGI

MGI:1101059. Tnfrsf9.

Phylogenomic databases

HOGENOM

P20334.

HOVERGEN

P20334.

Gene expression databases

ArrayExpress

P20334.

GermOnline

ENSMUSG00000028965. Mus musculus.

Family and domain databases

InterPro

IPR001368. TNFR_c6.
[Graphical view]

Pfam

PF00020. TNFR_c6. 1 hit.
[Graphical view]

SMART

SM00208. TNFR. 2 hits.
[Graphical view]

PROSITE

PS00652. TNFR_NGFR_1. 1 hit.
PS50050. TNFR_NGFR_2. False negative.
[Graphical view]

ProDom

P20334.
[Graphical view] [Entries sharing at least one domain]

BLOCKS

Search...

Other Resources

SOURCE

Search...

ProtoNet

Search...

Entry information

Entry name

TNR9_MOUSE

Accession

Primary (citable) accession number: P20334

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	July 22, 2008
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)