Reviewed, UniProtKB/Swiss-Prot P20458 (IF1_BACSU)

Names and origin

Protein names

Recommended name:
    Translation initiation factor IF-1

Gene names

Name:	infA
Ordered Locus Names:	BSU01390

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	72 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	No specific function has so far been attributed to this initiation factor; however, it seems to stimulate more or less all the activities of the other two initiation factors, IF-2 and IF-3.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the IF-1 family. Contains 1 S1-like domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Molecular function	Initiation factor
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	translational initiation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	RNA binding Inferred from electronic annotation. Source: HAMAP translation initiation factor activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 72	71	Translation initiation factor IF-1		PRO_0000095738
Regions
	Domain	2 – 72	71	S1-like
Amino acid modifications
	Modified residue	60	1	Phosphotyrosine

Sequences

	Sequence		Length	Mass (Da)
	P20458-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 57D35BBC6C196291	FASTA	72	8,214
	10 20 30 40 50 60 MAKDDVIEVE GTIVETLPNA MFKVELENGH TVLAHVSGKI RMHFIRILPG DKVTVELSPY 70 DLTRGRITYR YK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Gene encoding the alpha core subunit of Bacillus subtilis RNA polymerase is cotranscribed with the genes for initiation factor 1 and ribosomal proteins B, S13, S11, and L17." Boylan S.A., Suh J.-W., Thomas S.M., Price C.W. J. Bacteriol. 171:2553-2562(1989) [PubMed: 2496109] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Genetic and transcriptional organization of the Bacillus subtilis spc-alpha region." Suh J.-W., Boylan S.A., Oh S.H., Price C.W. Gene 169:17-23(1996) [PubMed: 8635744] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168 / Marburg.
[3]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[4]	"The serine/threonine/tyrosine phosphoproteome of the model bacterium Bacillus subtilis." Macek B., Mijakovic I., Olsen J.V., Gnad F., Kumar C., Jensen P.R., Mann M. Mol. Cell. Proteomics 6:697-707(2007) [PubMed: 17218307] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-60, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M26414 Genomic DNA. Translation: AAA22213.1. L47971 Genomic DNA. Translation: AAB06822.1. Z99104 Genomic DNA. Translation: CAB11915.1.
PIR	F69644.
RefSeq	NP_388020.1.
3D structure databases
HSSP	HSSP built from PDB template 1AH9 based on UniProtKB P02998.
ModBase	Search...
Genome annotation databases
GeneID	938924.
GenomeReviews	Gene locus BSU01390 in contig AL009126_GR.
KEGG	bsu:BSU01390.
NMPDR	fig|224308.1.peg.139.
Organism-specific databases
SubtiList	BG11043. infA. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	P20458.
Enzyme and pathway databases
BioCyc	BSUB224308:BSU0139-MON.
Family and domain databases
HAMAP	MF_00075. [Tree]
InterPro	IPR006196. S1_IF1. IPR003029. S1_RNA-bd. IPR004368. TIF_IF1. [Graphical view]
Pfam	PF01176. eIF-1a. 1 hit. [Graphical view]
SMART	SM00316. S1. 1 hit. [Graphical view]
TIGRFAMs	TIGR00008. infA. 1 hit.
PROSITE	PS50832. S1_IF1_TYPE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IF1_BACSU

Accession

Primary (citable) accession number: P20458

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	January 23, 2007
Last modified:	November 4, 2008
This is version 70 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)