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P21865

- KDPD_ECOLI

UniProt

P21865 - KDPD_ECOLI

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Protein
Sensor protein KdpD
Gene
kdpD, b0695, JW0683
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system KdpD/KdpE involved in the regulation of the kdp operon. KdpD may function as a membrane-associated protein kinase that phosphorylates KdpE in response to environmental signals.1 Publication

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

GO - Molecular functioni

  1. ATP bindingInferred from electronic annotationi Source: UniProtKB-KW
  2. aspartate kinase activityInferred from direct assayi PubMed 8276778 Source: EcoCyc
  3. phosphorelay sensor kinase activityInferred from direct assayi PubMed 8276778 Source: EcoCyc
  4. protein bindingInferred from physical interactioni PubMed 19400808PubMed 24561554 Source: IntAct
Complete GO annotation...

GO - Biological processi

  1. detection of chemical stimulusInferred from direct assayi PubMed 8276778 Source: EcoCyc
  2. osmosensory signaling via phosphorelay pathwayInferred from direct assayi PubMed 8276778 Source: EcoCyc
  3. protein autophosphorylationInferred from direct assayi PubMed 8223625 Source: EcoCyc
  4. signal transduction by phosphorylationInferred from direct assayi PubMed 8276778 Source: GOC
  1. aspartate kinase activityInferred from direct assayi PubMed 8276778 Source: EcoCyc
  2. phosphorelay sensor kinase activityInferred from direct assayi PubMed 8276778 Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Two-component regulatory system

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:KDPD-MONOMER.
ECOL316407:JW0683-MONOMER.
RETL1328306-WGS:GSTH-6122-MONOMER.
BRENDAi2.7.13.3. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Sensor protein KdpD (EC:2.7.13.3)
Gene namesi
Name:kdpD
Ordered Locus Names:b0695, JW0683
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10516. kdpD.

Subcellular locationi

Cell inner membrane; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 400400Cytoplasmic Inferred
Add
BLAST
Transmembranei401 – 42323Helical; Inferred
Add
BLAST
Topological domaini424 – 4274Periplasmic Inferred
Transmembranei428 – 44417Helical; Inferred
Add
BLAST
Topological domaini445 – 4451Cytoplasmic Inferred
Transmembranei446 – 46621Helical; Inferred
Add
BLAST
Topological domaini467 – 4748Periplasmic Inferred
Transmembranei475 – 49824Helical; Inferred
Add
BLAST
Topological domaini499 – 894396Cytoplasmic Inferred
Add
BLAST

GO - Cellular componenti

  1. integral component of membraneInferred from electronic annotationi Source: UniProtKB-KW
  2. intrinsic component of the cytoplasmic side of the plasma membraneInferred from direct assayi PubMed 1532388 Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 894894Sensor protein KdpD
PRO_0000074772Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei673 – 6731Phosphohistidine; by autocatalysis By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP21865.
PRIDEiP21865.

Expressioni

Gene expression databases

GenevestigatoriP21865.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
kdpEP218665EBI-1123100,EBI-6403634
ptsNP698294EBI-1123100,EBI-547017

Protein-protein interaction databases

DIPiDIP-10062N.
IntActiP21865. 5 interactions.
STRINGi511145.b0695.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi399 – 4013
Helixi402 – 42019
Helixi430 – 44314
Helixi450 – 46112
Helixi475 – 49824

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2KSFNMR-A397-502[»]
ProteinModelPortaliP21865.
SMRiP21865. Positions 22-227, 397-502, 599-882.

Miscellaneous databases

EvolutionaryTraceiP21865.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini670 – 883214Histidine kinase
Add
BLAST

Sequence similaritiesi

In the central section; belongs to the universal stress protein A family.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG2205.
HOGENOMiHOG000082933.
KOiK07646.
OMAiETPRLHR.
OrthoDBiEOG6G4VQG.
PhylomeDBiP21865.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
3.40.50.300. 1 hit.
3.40.50.620. 1 hit.
InterProiIPR025201. DUF4118.
IPR003661. EnvZ-like_dim/P.
IPR003018. GAF.
IPR003594. HATPase_ATP-bd.
IPR027417. P-loop_NTPase.
IPR014729. Rossmann-like_a/b/a_fold.
IPR004358. Sig_transdc_His_kin-like_C.
IPR005467. Sig_transdc_His_kinase_core.
IPR009082. Sig_transdc_His_kinase_dimeric.
IPR003852. Sig_transdc_His_kinase_KdpD_N.
IPR006016. UspA.
[Graphical view]
PfamiPF13493. DUF4118. 1 hit.
PF13492. GAF_3. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF02702. KdpD. 1 hit.
PF00582. Usp. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P21865-1 [UniParc]FASTA

« Hide

MNNEPLRPDP DRLLEQTAAP HRGKLKVFFG ACAGVGKTWA MLAEAQRLRA    50
QGLDIVVGVV ETHGRKDTAA MLEGLAVLPL KRQAYRGRHI SEFDLDAALA 100
RRPALILMDE LAHSNAPGSR HPKRWQDIEE LLEAGIDVFT TVNVQHLESL 150
NDVVSGVTGI QVRETVPDPF FDAADDVVLV DLPPDDLRQR LKEGKVYIAG 200
QAERAIEHFF RKGNLIALRE LALRRTADRV DEQMRAWRGH PGEEKVWHTR 250
DAILLCIGHN TGSEKLVRAA ARLASRLGSV WHAVYVETPA LHRLPEKKRR 300
AILSALRLAQ ELGAETATLS DPAEEKAVVR YAREHNLGKI ILGRPASRRW 350
WRRETFADRL ARIAPDLDQV LVALDEPPAR TINNAPDNRS FKDKWRVQIQ 400
GCVVAAALCA VITLIAMQWL MAFDAANLVM LYLLGVVVVA LFYGRWPSVV 450
ATVINVVSFD LFFIAPRGTL AVSDVQYLLT FAVMLTVGLV IGNLTAGVRY 500
QARVARYREQ RTRHLYEMSK ALAVGRSPQD IAATSEQFIA STFHARSQVL 550
LPDDNGKLQP LTHPQGMTPW DDAIAQWSFD KGLPAGAGTD TLPGVPYQIL 600
PLKSGEKTYG LVVVEPGNLR QLMIPEQQRL LETFTLLVAN ALERLTLTAS 650
EEQARMASER EQIRNALLAA LSHDLRTPLT VLFGQAEILT LDLASEGSPH 700
ARQASEIRQH VLNTTRLVNN LLDMARIQSG GFNLKKEWLT LEEVVGSALQ 750
MLEPGLSSPI NLSLPEPLTL IHVDGPLFER VLINLLENAV KYAGAQAEIG 800
IDAHVEGENL QLDVWDNGPG LPPGQEQTIF DKFARGNKES AVPGVGLGLA 850
ICRAIVDVHG GTITAFNRPE GGACFRVTLP QQTAPELEEF HEDM 894
Length:894
Mass (Da):98,718
Last modified:February 1, 1996 - v2
Checksum:iE9D2749B4CAA12DA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M36066 Genomic DNA. Translation: AAA24041.1.
U00096 Genomic DNA. Translation: AAC73789.1.
AP009048 Genomic DNA. Translation: BAA35352.1.
PIRiB42372.
RefSeqiNP_415223.1. NC_000913.3.
YP_488975.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC73789; AAC73789; b0695.
BAA35352; BAA35352; BAA35352.
GeneIDi12930931.
946744.
KEGGiecj:Y75_p0674.
eco:b0695.
PATRICi32116585. VBIEscCol129921_0725.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M36066 Genomic DNA. Translation: AAA24041.1 .
U00096 Genomic DNA. Translation: AAC73789.1 .
AP009048 Genomic DNA. Translation: BAA35352.1 .
PIRi B42372.
RefSeqi NP_415223.1. NC_000913.3.
YP_488975.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2KSF NMR - A 397-502 [» ]
ProteinModelPortali P21865.
SMRi P21865. Positions 22-227, 397-502, 599-882.
ModBasei Search...

Protein-protein interaction databases

DIPi DIP-10062N.
IntActi P21865. 5 interactions.
STRINGi 511145.b0695.

Proteomic databases

PaxDbi P21865.
PRIDEi P21865.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC73789 ; AAC73789 ; b0695 .
BAA35352 ; BAA35352 ; BAA35352 .
GeneIDi 12930931.
946744.
KEGGi ecj:Y75_p0674.
eco:b0695.
PATRICi 32116585. VBIEscCol129921_0725.

Organism-specific databases

EchoBASEi EB0511.
EcoGenei EG10516. kdpD.

Phylogenomic databases

eggNOGi COG2205.
HOGENOMi HOG000082933.
KOi K07646.
OMAi ETPRLHR.
OrthoDBi EOG6G4VQG.
PhylomeDBi P21865.

Enzyme and pathway databases

BioCyci EcoCyc:KDPD-MONOMER.
ECOL316407:JW0683-MONOMER.
RETL1328306-WGS:GSTH-6122-MONOMER.
BRENDAi 2.7.13.3. 2026.

Miscellaneous databases

EvolutionaryTracei P21865.
PROi P21865.

Gene expression databases

Genevestigatori P21865.

Family and domain databases

Gene3Di 1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
3.40.50.300. 1 hit.
3.40.50.620. 1 hit.
InterProi IPR025201. DUF4118.
IPR003661. EnvZ-like_dim/P.
IPR003018. GAF.
IPR003594. HATPase_ATP-bd.
IPR027417. P-loop_NTPase.
IPR014729. Rossmann-like_a/b/a_fold.
IPR004358. Sig_transdc_His_kin-like_C.
IPR005467. Sig_transdc_His_kinase_core.
IPR009082. Sig_transdc_His_kinase_dimeric.
IPR003852. Sig_transdc_His_kinase_KdpD_N.
IPR006016. UspA.
[Graphical view ]
Pfami PF13493. DUF4118. 1 hit.
PF13492. GAF_3. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF02702. KdpD. 1 hit.
PF00582. Usp. 1 hit.
[Graphical view ]
PRINTSi PR00344. BCTRLSENSOR.
SMARTi SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
[Graphical view ]
SUPFAMi SSF47384. SSF47384. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEi PS50109. HIS_KIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "KdpD and KdpE, proteins that control expression of the kdpABC operon, are members of the two-component sensor-effector class of regulators."
    Walderhaug M.O., Polarek J.W., Voelkner P., Daniel J.M., Hesse J.E., Altendorf K., Epstein W.
    J. Bacteriol. 174:2152-2159(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
    Strain: K12.
  2. "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map."
    Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K.
    , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T.
    DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "The complete genome sequence of Escherichia coli K-12."
    Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
    Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Membrane topology analysis of the sensor kinase KdpD of Escherichia coli."
    Zimmann P., Puppe W., Altendorf K.
    J. Biol. Chem. 270:28282-28288(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY.
  6. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.

Entry nameiKDPD_ECOLI
AccessioniPrimary (citable) accession number: P21865
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: February 1, 1996
Last modified: July 9, 2014
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene

External Data

Dasty 3

Similar proteinsi